A0A0M4VB73 · A0A0M4VB73_9EUTH
- ProteinV(D)J recombination-activating protein 1
- GeneRAG1
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids336 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends.
Catalytic activity
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 divalent metal cation per subunit. Mg2+ or Mn2+.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | DNA recombinase complex | |
Cellular Component | endodeoxyribonuclease complex | |
Cellular Component | nucleus | |
Molecular Function | double-stranded DNA endonuclease activity | |
Molecular Function | histone binding | |
Molecular Function | protein homodimerization activity | |
Molecular Function | sequence-specific DNA binding | |
Molecular Function | ubiquitin protein ligase activity | |
Molecular Function | zinc ion binding | |
Biological Process | adaptive immune response | |
Biological Process | chromatin organization | |
Biological Process | pre-B cell allelic exclusion | |
Biological Process | T cell differentiation in thymus | |
Biological Process | V(D)J recombination |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameV(D)J recombination-activating protein 1
- EC number
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Eulipotyphla > Talpidae > Euroscaptor
Accessions
- Primary accessionA0A0M4VB73
Subcellular Location
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Sequence
- Sequence statusFragment
- Length336
- Mass (Da)38,340
- Last updated2015-12-09 v1
- Checksum76561AAB2EEAF1BC
Features
Showing features for non-terminal residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Non-terminal residue | 1 | |||||
Sequence: S | ||||||
Non-terminal residue | 336 | |||||
Sequence: T |
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
KP995375 EMBL· GenBank· DDBJ | ALN43286.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KX164278 EMBL· GenBank· DDBJ | AQM57842.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KX164279 EMBL· GenBank· DDBJ | AQM57843.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KX164280 EMBL· GenBank· DDBJ | AQM57844.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KX164281 EMBL· GenBank· DDBJ | AQM57845.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KX164282 EMBL· GenBank· DDBJ | AQM57846.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KX164283 EMBL· GenBank· DDBJ | AQM57847.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KX164285 EMBL· GenBank· DDBJ | AQM57849.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LC013317 EMBL· GenBank· DDBJ | BAS50165.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LC013318 EMBL· GenBank· DDBJ | BAS50166.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LC013319 EMBL· GenBank· DDBJ | BAS50167.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LC013320 EMBL· GenBank· DDBJ | BAS50168.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LC013321 EMBL· GenBank· DDBJ | BAS50169.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LC013322 EMBL· GenBank· DDBJ | BAS50170.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LC013323 EMBL· GenBank· DDBJ | BAS50171.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LC013324 EMBL· GenBank· DDBJ | BAS50172.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LC013325 EMBL· GenBank· DDBJ | BAS50173.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LC013327 EMBL· GenBank· DDBJ | BAS50175.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LC013328 EMBL· GenBank· DDBJ | BAS50176.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LC013329 EMBL· GenBank· DDBJ | BAS50177.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LC013330 EMBL· GenBank· DDBJ | BAS50178.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LC013331 EMBL· GenBank· DDBJ | BAS50179.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LC013332 EMBL· GenBank· DDBJ | BAS50180.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LC013333 EMBL· GenBank· DDBJ | BAS50181.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LC013334 EMBL· GenBank· DDBJ | BAS50182.1 EMBL· GenBank· DDBJ | Genomic DNA |