A0A0M4VB73 · A0A0M4VB73_9EUTH

  • Protein
    V(D)J recombination-activating protein 1
  • Gene
    RAG1
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends.

Catalytic activity

  • S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.
    EC:2.3.2.27 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds 1 divalent metal cation per subunit. Mg2+ or Mn2+.

GO annotations

AspectTerm
Cellular ComponentDNA recombinase complex
Cellular Componentendodeoxyribonuclease complex
Cellular Componentnucleus
Molecular Functiondouble-stranded DNA endonuclease activity
Molecular Functionhistone binding
Molecular Functionprotein homodimerization activity
Molecular Functionsequence-specific DNA binding
Molecular Functionubiquitin protein ligase activity
Molecular Functionzinc ion binding
Biological Processadaptive immune response
Biological Processchromatin organization
Biological Processpre-B cell allelic exclusion
Biological ProcessT cell differentiation in thymus
Biological ProcessV(D)J recombination

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    V(D)J recombination-activating protein 1
  • EC number

Gene names

    • Name
      RAG1

Organism names

  • Taxonomic identifier
  • Organism
  • Strains
    • IEBR-M-1734/SIK0875
    • IEBR-M-1735/SIK0876
    • IEBR-M-1798/SIK0882
    • IEBR-M-1799/SIK0883
    • IEBR-M-3250/SIK0913
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Eulipotyphla > Talpidae > Euroscaptor

Accessions

  • Primary accession
    A0A0M4VB73

Subcellular Location

Keywords

  • Cellular component

Interaction

Subunit

Homodimer.

Family & Domains

Sequence similarities

Belongs to the RAG1 family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Fragment
  • Length
    336
  • Mass (Da)
    38,340
  • Last updated
    2015-12-09 v1
  • Checksum
    76561AAB2EEAF1BC
SCDGMGDVSEKHGSGPAVPEKAVRFSFTVMKITIAHGSQNVQVFEEAKPNSELCCKPLCLMLADESDHETLTAILSPLIAEREAMKSSQLMLEMGGILRTFKFIFRGTGYDEKLVREVEGLEASGSVYICTLCDATRLEASQNLVFHSITRSHTENLERYEVWRSNPYHESVEELRDRVKGVSAKPFIETVPSIDALHCDIGNAAEFYKIFQLEIGEVYKNPNASKEERKRWQATLDKHLRKKMNLKPIMRMNGNFARKLMTKETVEAVCELIPSEERHAALRELMDLYLKMKPVWRSSCPAKECPESLCQYSFNSQRFAELLSTKFKYRYEGKIT

Features

Showing features for non-terminal residue.

TypeIDPosition(s)Description
Non-terminal residue1
Non-terminal residue336

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KP995375
EMBL· GenBank· DDBJ
ALN43286.1
EMBL· GenBank· DDBJ
Genomic DNA
KX164278
EMBL· GenBank· DDBJ
AQM57842.1
EMBL· GenBank· DDBJ
Genomic DNA
KX164279
EMBL· GenBank· DDBJ
AQM57843.1
EMBL· GenBank· DDBJ
Genomic DNA
KX164280
EMBL· GenBank· DDBJ
AQM57844.1
EMBL· GenBank· DDBJ
Genomic DNA
KX164281
EMBL· GenBank· DDBJ
AQM57845.1
EMBL· GenBank· DDBJ
Genomic DNA
KX164282
EMBL· GenBank· DDBJ
AQM57846.1
EMBL· GenBank· DDBJ
Genomic DNA
KX164283
EMBL· GenBank· DDBJ
AQM57847.1
EMBL· GenBank· DDBJ
Genomic DNA
KX164285
EMBL· GenBank· DDBJ
AQM57849.1
EMBL· GenBank· DDBJ
Genomic DNA
LC013317
EMBL· GenBank· DDBJ
BAS50165.1
EMBL· GenBank· DDBJ
Genomic DNA
LC013318
EMBL· GenBank· DDBJ
BAS50166.1
EMBL· GenBank· DDBJ
Genomic DNA
LC013319
EMBL· GenBank· DDBJ
BAS50167.1
EMBL· GenBank· DDBJ
Genomic DNA
LC013320
EMBL· GenBank· DDBJ
BAS50168.1
EMBL· GenBank· DDBJ
Genomic DNA
LC013321
EMBL· GenBank· DDBJ
BAS50169.1
EMBL· GenBank· DDBJ
Genomic DNA
LC013322
EMBL· GenBank· DDBJ
BAS50170.1
EMBL· GenBank· DDBJ
Genomic DNA
LC013323
EMBL· GenBank· DDBJ
BAS50171.1
EMBL· GenBank· DDBJ
Genomic DNA
LC013324
EMBL· GenBank· DDBJ
BAS50172.1
EMBL· GenBank· DDBJ
Genomic DNA
LC013325
EMBL· GenBank· DDBJ
BAS50173.1
EMBL· GenBank· DDBJ
Genomic DNA
LC013327
EMBL· GenBank· DDBJ
BAS50175.1
EMBL· GenBank· DDBJ
Genomic DNA
LC013328
EMBL· GenBank· DDBJ
BAS50176.1
EMBL· GenBank· DDBJ
Genomic DNA
LC013329
EMBL· GenBank· DDBJ
BAS50177.1
EMBL· GenBank· DDBJ
Genomic DNA
LC013330
EMBL· GenBank· DDBJ
BAS50178.1
EMBL· GenBank· DDBJ
Genomic DNA
LC013331
EMBL· GenBank· DDBJ
BAS50179.1
EMBL· GenBank· DDBJ
Genomic DNA
LC013332
EMBL· GenBank· DDBJ
BAS50180.1
EMBL· GenBank· DDBJ
Genomic DNA
LC013333
EMBL· GenBank· DDBJ
BAS50181.1
EMBL· GenBank· DDBJ
Genomic DNA
LC013334
EMBL· GenBank· DDBJ
BAS50182.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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