A0A0M4M099 · A0A0M4M099_9ACTN

Function

function

Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds 1 Mg2+ or Mn2+ ion per subunit.
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.

Features

Showing features for binding site, site.

TypeIDPosition(s)Description
Binding site88substrate
Binding site98substrate
Binding site122substrate
Site129Important for catalysis
Site179Important for catalysis
Binding site212Mg2+ (UniProtKB | ChEBI)
Binding site212substrate
Binding site236Mg2+ (UniProtKB | ChEBI)
Binding site240Mg2+ (UniProtKB | ChEBI)
Binding site272-284NAD+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function3-isopropylmalate dehydrogenase activity
Molecular Functionmagnesium ion binding
Molecular FunctionNAD binding
Biological ProcessL-leucine biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    3-isopropylmalate dehydrogenase
  • EC number
  • Alternative names
    • 3-IPM-DH
    • Beta-IPM dehydrogenase
      (IMDH
      )

Gene names

    • Name
      leuB
    • ORF names
      AL705_01355

Organism names

  • Taxonomic identifier
  • Strain
    • X1698
  • Taxonomic lineage
    Bacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Lawsonellaceae > Lawsonella

Accessions

  • Primary accession
    A0A0M4M099

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain2-333Isopropylmalate dehydrogenase-like

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    338
  • Mass (Da)
    35,916
  • Last updated
    2015-12-09 v1
  • Checksum
    D38051B6754E60EC
MNLAVIGGDGIGPEVTAEALKVLHTVVPNIEVTDYALGADHYLETGEILSDETVESIRQQDAILLGAIGDPARVAPGVLERGLLLKLRFALDHFVNLRPAKLYAGVESPLAGNPALDFVVVREGTEGLYCGDGGAIRVGTPAEVATETSLNTRFGVERVVRDAFERAMKRNKRLTLVHKMNVLTNAGSLWKRTVDEVSVEYPEVTTDYCHIDACTIYMVTDPGRFDVIVTDNLFGDIITDLAGAVMGGIGLAASGNIDATRTNPSMFEPVHGSAPDIAGKGLADPTAAIISAAMLLEHLGYDEEATRIMQVVEEDVAHRGDGPISTSAVGDRIVAALQ

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP012390
EMBL· GenBank· DDBJ
ALE19557.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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