A0A0M2UV62 · A0A0M2UV62_9BACT

  • Protein
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase
  • Gene
    ispH
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster per subunit.

Pathway

Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1.
Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site46[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site77substrate
Binding site109substrate
Binding site131[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site159substrate
Active site161Proton donor
Binding site197substrate
Binding site225[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site253-255substrate
Binding site297substrate

GO annotations

AspectTerm
Cellular Componentmembrane
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Function4-hydroxy-3-methylbut-2-enyl diphosphate reductase activity
Molecular Functionmetal ion binding
Molecular Functiontransferase activity, transferring alkyl or aryl (other than methyl) groups
Biological Processdimethylallyl diphosphate biosynthetic process
Biological Processisopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase
  • EC number
  • Short names
    HMBPP reductase

Gene names

    • Name
      ispH
    • ORF names
      BROFUL_02427

Organism names

  • Taxonomic identifier
  • Strain
    • RU1
  • Taxonomic lineage
    Bacteria > Planctomycetota > Candidatus Brocadiia > Candidatus Brocadiales > Candidatus Brocadiaceae > Candidatus Brocadia

Accessions

  • Primary accession
    A0A0M2UV62

Proteomes

Subcellular Location

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane328-353Helical
Transmembrane359-380Helical
Transmembrane401-418Helical
Transmembrane424-444Helical
Transmembrane465-482Helical
Transmembrane488-509Helical
Transmembrane537-555Helical
Transmembrane561-580Helical
Transmembrane589-609Helical

Keywords

  • Cellular component

Family & Domains

Sequence similarities

Belongs to the IspH family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    614
  • Mass (Da)
    68,119
  • Last updated
    2015-11-11 v1
  • Checksum
    2CA9F6E0473207D5
MRDAHGNTEIVSGVLHREPFFVKIFSKYRIRGILLRVKVAKTAGFCMGVRRAMDILLDAANEKNDDGNVFTDGPLIHNPQVLEYLEKRGIQVVKGQTDLSKSTVVIRAHGVTPTRRKEIESTGAKVCDATCPHVMRVQSIIKKYAAQGYSTVIVGDKGHAEVIGLLGYAEGKGYVVQELDEIEHLPPMDKVCIVAQTTQDRRMFKEAIERLKKRYANCESFETICSSTYKRQDEIISLSKTVDAMIVVGGRGSANTTRLVKICESQGTPTFLVETDAELDLEKLKDYDTVGVTAGASTPNWMIKRVVEKVHSFKLSRYRRFLYGMRGIASYFIRSCTYAGLGAASLSYASAVLLGIRPRISFCLIAALFIFSMQVLNRFSNKEAIALNEPSGAKFYEKKQALFICLGIAGVVASFALGFVLNKSIFFCTFLAGLIGIFYRLEIIPRSLSRIIRYRSLEQIPGSKEIFYSLAWAVSTTLIPFLGAKQSFTPSLVIASAFAFSLAFIRAVVLNIRDIQGDRILGKETIPIAIGKERTKTILIIITALVAVLMSASPWLGWTTSLGYCLLPCVAYASGYQYLFQKRIVSEGLLSETITDFNFILAGIIAFVWKSNHF

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
LAQJ01000231
EMBL· GenBank· DDBJ
KKO18881.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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