A0A0M2UU87 · A0A0M2UU87_9BACT

Function

function

A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.

Miscellaneous

Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • ATP-dependent breakage, passage and rejoining of double-stranded DNA.
    EC:5.6.2.2 (UniProtKB | ENZYME | Rhea)

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site122O-(5'-phospho-DNA)-tyrosine intermediate

GO annotations

AspectTerm
Cellular Componentchromosome
Cellular Componentcytoplasm
Cellular ComponentDNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex
Molecular FunctionATP binding
Molecular FunctionDNA binding
Molecular FunctionDNA negative supercoiling activity
Biological ProcessDNA topological change
Biological ProcessDNA-templated DNA replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA gyrase subunit A
  • EC number

Gene names

    • Name
      gyrA
    • ORF names
      BROFUL_01711

Organism names

  • Taxonomic identifier
  • Strain
    • RU1
  • Taxonomic lineage
    Bacteria > Planctomycetota > Candidatus Brocadiia > Candidatus Brocadiales > Candidatus Brocadiaceae > Candidatus Brocadia

Accessions

  • Primary accession
    A0A0M2UU87

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.

Family & Domains

Features

Showing features for domain, motif, compositional bias, region.

TypeIDPosition(s)Description
Domain11-464DNA topoisomerase type IIA
Motif525-531GyrA-box
Compositional bias808-826Basic and acidic residues
Region808-839Disordered

Sequence similarities

Belongs to the type II topoisomerase GyrA/ParC subunit family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    839
  • Mass (Da)
    94,112
  • Last updated
    2015-11-11 v1
  • Checksum
    4EC109804DDA7807
MVERRENIKELFIEEEMKDSYLSYAMSVIMSRALPDVRDGLKPSQRRILVAMNDLGLGPRSKFRKCAKIAGDTTGNYHPHGEQVVYPTLVRMAQDFNYRYPLIEGQGNFGSIDGDPPAAMRYTEARMTEATMIIMEDLERETVDYVPNYDDTRTEPIVLPSKFPNLLCNGCSGIAVGMATSVPPHNVNEICDGIVAVIDNPEITVDELMKIIKGPDFPTGALICGTQGIRDGYRTGRGTIIVRARAHTETSKNGKKSIVVTEIPYQLNRDNILERIAELVRDDQIKGISDIRNESDREGSRLVIDLKKGEDEEVVLNQLYKHTKLQDSFSIIMIALINNRPETLNLKQMLVYYIEHRKVVIIRRTKYLLEKAQARAHILEGLRIALQNIDRIIQLIKTSDSIETARRGLMSEFSLSELQANAILDMKLQRLTGLEQGKIEEEYKKLCADIRGYQAILANEKLVFDLIKKDVDEVKERFGDKRRTEIVGAVTELNMEDLIAEESVAVIISHEGYIKRLPLTSYRKQHRGGKGVSGADMKEGDFIEHLFVASTHDYILFFTDQGRVYWLKVYDVPQMARTAKGRALINLLELKEDENVTSLIPVRDFDERQLVMATRNGIVKKTVLSAYGNPKKGGIIAINLDEGDKLIGVKLTHGKQDIILGTEQGKAMRFSEEDVRTMGRVTHGVKGITLKEDDKVRDIVIVDEQATLLTVCEKGFGKRTDFAEYPVHRRGGLGVINIKTTERNGKVVALIDVRDEDELIMITARGQVIRTPVNTIRSIGRNTQGVTLFSIEDGDKLVSVARVAPEEVKAGDNAEEAARAGADTEEIPDESPQEDGDEG

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias808-826Basic and acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
LAQJ01000176
EMBL· GenBank· DDBJ
KKO19603.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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