A0A0M2UQK3 · A0A0M2UQK3_9BACT

  • Protein
    Phosphoribosylformylglycinamidine synthase subunit PurL
  • Gene
    purL
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide: step 1/2.

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site231
Binding site290ATP (UniProtKB | ChEBI)
Binding site292Mg2+ 1 (UniProtKB | ChEBI)
Active site294Proton acceptor
Binding site315substrate
Binding site316Mg2+ 2 (UniProtKB | ChEBI)
Binding site449substrate
Binding site477Mg2+ 2 (UniProtKB | ChEBI)
Binding site709ATP (UniProtKB | ChEBI)
Binding site746ATP (UniProtKB | ChEBI)
Binding site749substrate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular Functionmagnesium ion binding
Molecular Functionphosphoribosylformylglycinamidine synthase activity
Biological Process'de novo' IMP biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Phosphoribosylformylglycinamidine synthase subunit PurL
  • EC number
  • Short names
    FGAM synthase
  • Alternative names
    • Formylglycinamide ribonucleotide amidotransferase subunit II
      (FGAR amidotransferase II
      ; FGAR-AT II
      )
    • Glutamine amidotransferase PurL
    • Phosphoribosylformylglycinamidine synthase subunit II

Gene names

    • Name
      purL
    • ORF names
      BROFUL_03196

Organism names

  • Taxonomic identifier
  • Strain
    • RU1
  • Taxonomic lineage
    Bacteria > Planctomycetota > Candidatus Brocadiia > Candidatus Brocadiales > Candidatus Brocadiaceae > Candidatus Brocadia

Accessions

  • Primary accession
    A0A0M2UQK3

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain186-235Phosphoribosylformylglycinamidine synthase linker
Domain274-399PurM-like N-terminal
Domain410-563PurM-like C-terminal
Domain651-750PurM-like N-terminal
Domain788-931PurM-like C-terminal

Sequence similarities

Belongs to the FGAMS family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    958
  • Mass (Da)
    105,570
  • Last updated
    2015-11-11 v1
  • Checksum
    5ED6FFC3B461D554
MYSRIEVFFKDAFPDPLGNTIRSEIEAFGFQGITNVRVCQVYILFGKVRKNDLDTIAQKLLTDTITQHYECNPAIFTKIGANGRSPLHIVEISRKPGVMDPVEQSVLKAIRDMGIDVEGAKTAQKYLIDGNISDEAIHNIATRILANTKIEDVFVYPETPNYNHGTLQYTLKKLTVPLLQADDKQLEEISIRGQLSLNIPEMQSIQRYYQSLKREPTDVELETIAQTWSEHCFHKTFKGIIDFNGNTIDNLLQHTIMKATKELNKPWCVSVFKDNAGIIDFDNTYNVCFKVETHNHPSAIEPYGGANTGIGGVIRDIMGTGLGGKPILNTDVFCFGIPDTPADKIPKGVLHPKRIFKGVIAGVRDYGNRMGIPTANGAIFFDERYLGNPIVFCGTVGLIPKNMCQKEAHPHDLIVVAGGRTGRDGIHGATFSSAELHEQSEQMSGGAVQIGNAIAEKALLDVLLQARDKDLYHCITDCGAGGLSSAVGEMGQDLGAMVNLEKVPLKYEGLSYTEIWISEAQERMVLAVPPESEHEILELFKSENVEATVIGRFTNDKILRLFYHSHLVGEIGMDFLHKGIPRLERKATWHKPLLEEPALPEKEDFGTDLKKILSSWNVCSKEWVIRQYDHEVQGGSVLKPLHGLHNDGPGDACMITPVLGSTKGIIVSNGMNPRYGDIDPYHMAASAIDEALRQIIAVGGNLGQVALLDNFCWGNTNKPDRLGSLVRAAQACYDIAITYGTPFISGKDSLNNEFVTDKETIVIPPTLLISAVSVIEDVRNAVSMDIKEPGNLVYIIGITRPELGGSHYYHIHGYKGNNVPKVDAVLGKNVMHAVSQATSQRIIRSCHDCSEGGLAVAAAEMAFAGGYGMSLNLSGVVTEGPVHRNDTILFSESNSRFIVEVRPEHQKQFETLMKDIPCGLLGKVTSEPLLKIYGLTNKLIVSENIYDLKDAWQAPLRW

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
LAQJ01000292
EMBL· GenBank· DDBJ
KKO18147.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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