A0A0M2UPQ1 · A0A0M2UPQ1_9BACT

Function

function

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide.
    EC:6.5.1.2 (UniProtKB | ENZYME | Rhea)

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site32-36NAD+ (UniProtKB | ChEBI)
Binding site81-82NAD+ (UniProtKB | ChEBI)
Binding site115NAD+ (UniProtKB | ChEBI)
Active site117N6-AMP-lysine intermediate
Binding site138NAD+ (UniProtKB | ChEBI)
Binding site179NAD+ (UniProtKB | ChEBI)
Binding site295NAD+ (UniProtKB | ChEBI)
Binding site319NAD+ (UniProtKB | ChEBI)
Binding site413Zn2+ (UniProtKB | ChEBI)
Binding site416Zn2+ (UniProtKB | ChEBI)
Binding site429Zn2+ (UniProtKB | ChEBI)
Binding site434Zn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular FunctionDNA binding
Molecular FunctionDNA ligase (NAD+) activity
Molecular Functionmetal ion binding
Biological Processbase-excision repair, DNA ligation
Biological ProcessDNA replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA ligase
  • EC number
  • Alternative names
    • Polydeoxyribonucleotide synthase [NAD(+)]

Gene names

    • Name
      ligA
    • ORF names
      BROFUL_03223

Organism names

  • Taxonomic identifier
  • Strain
    • RU1
  • Taxonomic lineage
    Bacteria > Planctomycetota > Candidatus Brocadiia > Candidatus Brocadiales > Candidatus Brocadiaceae > Candidatus Brocadia

Accessions

  • Primary accession
    A0A0M2UPQ1

Proteomes

Subcellular Location

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain594-671BRCT

Sequence similarities

Belongs to the NAD-dependent DNA ligase family. LigA subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    671
  • Mass (Da)
    75,879
  • Last updated
    2015-11-11 v1
  • Checksum
    C6DEFA4E8213C108
MIAINENIERLRDTIRRHDKKYYVENNPEITDYEYDQLIKELQQLEKAHPHLITPDSPTQRVGGEPLTQFSAINHRIPMLSIDNTYSDAELKEFDQRIKRMTGIDVHRDVEYVVELKIDGVAITLWYEKGLFVWGATRGDGFTGDDVTANLRTIRQIPLKLESVDRSQKIPSVVEIRGEIYLPNREFQRLNEAREESGEPQFANPRNAAAGTLKLLDPRITAKRRLRIFRYAIGHVEDSELSTHLQCLELIRGFGLPVNPHTRVCENIEAVIQYCNDWEKKRRDLDYLVDGMVIKVNSLALHEQLGATSKAPRWVISYKYQPERAVTVIEEIVTQVGKTGTITPVANLSPVLLAGTMVSRATLHNFEEISRKDIRVGDHVVVQKAGEIIPQVVSVVKEKRDGTERTFQEPVMCPECNGSVQQDGVYRRCHNPFCPAQAKRRIIYFASRHAMDIEGFGPALVEQLVDKGLVKDYADIYHLKHQELTDLERMGEKSSSNVIRAIEESKQRGLDRLICALGIPNVGTHTAEVLAGHFDTLDALAHATHGALEGIYEIGPVVAKSIVEFFQDKHTQGILEKLKAAGVNIRRQTIPSSEKNPKISGKSFIITGTLQKHSRKEAEMLIKQQGGRIVSQVSKKTNYLVVGEDPGSKLDKARELDVAILDEETFEKMIR

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
LAQJ01000294
EMBL· GenBank· DDBJ
KKO18093.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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