A0A0M2UP73 · A0A0M2UP73_9BACT

Function

function

Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s2U34.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Features

Showing features for binding site, active site, site.

136150100150200250300350
TypeIDPosition(s)Description
Binding site8-15ATP (UniProtKB | ChEBI)
Binding site34ATP (UniProtKB | ChEBI)
Active site102Nucleophile
Binding site126ATP (UniProtKB | ChEBI)
Site127Interaction with tRNA
Active site198Cysteine persulfide intermediate
Site337Interaction with tRNA

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular Functionmethyltransferase activity
Molecular FunctiontRNA binding
Molecular FunctiontRNA-uridine 2-sulfurtransferase activity
Biological Processmethylation
Biological ProcesstRNA wobble position uridine thiolation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA-specific 2-thiouridylase MnmA
  • EC number

Gene names

    • Name
      mnmA
    • ORF names
      BROFUL_03384

Organism names

  • Taxonomic identifier
  • Strain
    • RU1
  • Taxonomic lineage
    Bacteria > Planctomycetota > Candidatus Brocadiia > Candidatus Brocadiales > Candidatus Brocadiaceae > Candidatus Brocadia

Accessions

  • Primary accession
    A0A0M2UP73

Proteomes

Subcellular Location

Keywords

PTM/Processing

Keywords

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region148-150Interaction with tRNA
Domain219-270tRNA-specific 2-thiouridylase MnmA-like central
Domain279-353tRNA-specific 2-thiouridylase MnmA-like C-terminal
Region304-305Interaction with tRNA

Sequence similarities

Belongs to the MnmA/TRMU family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    361
  • Mass (Da)
    40,160
  • Last updated
    2015-11-11 v1
  • Checksum
    2CBA765F8B3B55BB
MKAKVVVAMSGGVDSSVAAHLLVEQGYEVVGLFMRLGIDKLDTTTKTKICCSLEDAQDARTVADQLGIQFHVLNFKDAFNRIINTFCTEYLNGRTPNPCILCNQDLKFGRLLDFAKMLNADFIATGHYAKVEKACDRYLLKKGADSRKDQSYVLFSLNQEQLSRTIFPLGMVTKEAVREIARNLNLKTKDKPESQDVCFVLDNNYHTLLYERFGDRITPGLLKDTKGNVLGRHQGLPFFTIGQRKGLGVALGTPRYVVDINPQENTVVIGTDAELLENELVASAINWISIDKLDTPLNVQAKIRYHHVAADATVYPDKPDAVRVVFKEPQRAITPGQAVVFYDNDTVVGGGWIERKSGKTS

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
LAQJ01000310
EMBL· GenBank· DDBJ
KKO17933.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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