A0A0M2UP73 · A0A0M2UP73_9BACT
- ProteintRNA-specific 2-thiouridylase MnmA
- GenemnmA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids361 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s2U34.
Catalytic activity
- AH2 + ATP + S-sulfanyl-L-cysteinyl-[protein] + uridine34 in tRNA = 2-thiouridine34 in tRNA + A + AMP + diphosphate + H+ + L-cysteinyl-[protein]
CHEBI:17499 + CHEBI:30616 + RHEA-COMP:11726 + RHEA-COMP:11727 CHEBI:65315 Position: 34= RHEA-COMP:11728 CHEBI:87170 Position: 34+ CHEBI:13193 + CHEBI:456215 + CHEBI:33019 + CHEBI:15378 + RHEA-COMP:10131
Features
Showing features for binding site, active site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 8-15 | ATP (UniProtKB | ChEBI) | ||||
Sequence: AMSGGVDS | ||||||
Binding site | 34 | ATP (UniProtKB | ChEBI) | ||||
Sequence: M | ||||||
Active site | 102 | Nucleophile | ||||
Sequence: C | ||||||
Binding site | 126 | ATP (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Site | 127 | Interaction with tRNA | ||||
Sequence: H | ||||||
Active site | 198 | Cysteine persulfide intermediate | ||||
Sequence: C | ||||||
Site | 337 | Interaction with tRNA | ||||
Sequence: Q |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | methyltransferase activity | |
Molecular Function | tRNA binding | |
Molecular Function | tRNA-uridine 2-sulfurtransferase activity | |
Biological Process | methylation | |
Biological Process | tRNA wobble position uridine thiolation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA-specific 2-thiouridylase MnmA
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Planctomycetota > Candidatus Brocadiia > Candidatus Brocadiales > Candidatus Brocadiaceae > Candidatus Brocadia
Accessions
- Primary accessionA0A0M2UP73
Proteomes
Subcellular Location
PTM/Processing
Keywords
- PTM
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 148-150 | Interaction with tRNA | ||||
Sequence: KDQ | ||||||
Domain | 219-270 | tRNA-specific 2-thiouridylase MnmA-like central | ||||
Sequence: PGLLKDTKGNVLGRHQGLPFFTIGQRKGLGVALGTPRYVVDINPQENTVVIG | ||||||
Domain | 279-353 | tRNA-specific 2-thiouridylase MnmA-like C-terminal | ||||
Sequence: ELVASAINWISIDKLDTPLNVQAKIRYHHVAADATVYPDKPDAVRVVFKEPQRAITPGQAVVFYDNDTVVGGGWI | ||||||
Region | 304-305 | Interaction with tRNA | ||||
Sequence: RY |
Sequence similarities
Belongs to the MnmA/TRMU family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length361
- Mass (Da)40,160
- Last updated2015-11-11 v1
- Checksum2CBA765F8B3B55BB
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
LAQJ01000310 EMBL· GenBank· DDBJ | KKO17933.1 EMBL· GenBank· DDBJ | Genomic DNA |