A0A0L8RDM5 · A0A0L8RDM5_SACEU

Function

function

Core component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases. The complex 1 mediates the end resection of damaged DNA, which generates proper single-stranded DNA, a key initial steps in HR, and is 2 required for the recruitment of other repair factors and efficient activation of ATM and ATR upon DNA damage. Within the MRN complex, MRE11 possesses both single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity. MRE11 first endonucleolytically cleaves the 5' strand at DNA DSB ends to prevent non-homologous end joining (NHEJ) and licence HR. It then generates a single-stranded DNA gap via 3' to 5' exonucleolytic degradation, which is required for single-strand invasion and recombination.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site125Proton donor

GO annotations

AspectTerm
Cellular ComponentMre11 complex
Cellular Componentsite of double-strand break
Molecular Function3'-5'-DNA exonuclease activity
Molecular Functionmanganese ion binding
Molecular Functionsingle-stranded DNA endodeoxyribonuclease activity
Biological Processdouble-strand break repair via homologous recombination
Biological Processdouble-strand break repair via nonhomologous end joining
Biological Processmeiotic DNA double-strand break formation
Biological Processmitochondrial double-strand break repair via homologous recombination
Biological Processmitotic G2 DNA damage checkpoint signaling
Biological Processmitotic intra-S DNA damage checkpoint signaling
Biological Processtelomere maintenance

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Double-strand break repair protein

Gene names

    • ORF names
      DI49_4308

Organism names

  • Taxonomic identifier
  • Strain
    • FM1318
  • Taxonomic lineage
    Eukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces

Accessions

  • Primary accession
    A0A0L8RDM5

Proteomes

Subcellular Location

Keywords

Family & Domains

Features

Showing features for domain, region, compositional bias.

Type
IDPosition(s)Description
Domain291-474Mre11 DNA-binding
Region522-695Disordered
Compositional bias536-559Polar residues
Compositional bias560-588Basic and acidic residues
Compositional bias611-639Acidic residues

Sequence similarities

Belongs to the MRE11/RAD32 family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    695
  • Mass (Da)
    78,315
  • Last updated
    2015-11-11 v1
  • Checksum
    1E0670AA227FE04A
MEYPDPDTIRILVTTDNHVGYNENDPITGDDSWKTFHEVMMLAKNNNVDMVLQSGDLFHVNKPSKKSLYQVLKSLRLSCMGDKPCELELLSDPSQVFHYDEFTNVNYEDPNFNISIPVFGISGNHDDASGDSLLCPMDILHATGLINHFGKVIESDKIKIVPLLFQKGSTKLALYGLAAVRDERLFRTFKDGGVTFEVPTMREGEWFNLMCVHQNHTGHTNTAFLPEQFLPDFLDLVIWGHEHECIPNLVHNPMKGFDVLQPGSSVATSLCEAEAQPKYVFILEIKYGEAPKMTPIPLETIRTFKMRSISLQDVPHLRPHDKDVTSKYLIEQVEEMIEEANEETKRKLGDDADGDMISELPKPLIRLRVDYSAPPNKQSPIDYQVENPRRFSNRFVGRVANGNNVVQFYKRRSPATRSKNLGLNGTNISDRDVEKLLNESGGELEVQTLVNDLLNKMQLSLLPEVGLNEAVKKFVDKDEKTALKEFISHEISNEVDILSTNNEFLQTEDTEEMKALIKQVKRANSVRPATPMENDEASLSLDNNRLSSFQPSNELRRDKPSSTAQSHVSKEAEVDRTKEFESVKPTKKTRSTRTVRRKKTAAPSDSIVISDAENEFEDDNDARVDDVDVDEDDIIMVSTDEEDASYGLISGRKTKRKSRSPAGTKASSTRGKGRAKATRTPKTDILGSLLAKKRK

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias536-559Polar residues
Compositional bias560-588Basic and acidic residues
Compositional bias611-639Acidic residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JMCK01000013
EMBL· GenBank· DDBJ
KOG97554.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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