A0A0L8RDM5 · A0A0L8RDM5_SACEU
- ProteinDouble-strand break repair protein
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids695 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Core component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases. The complex 1 mediates the end resection of damaged DNA, which generates proper single-stranded DNA, a key initial steps in HR, and is 2 required for the recruitment of other repair factors and efficient activation of ATM and ATR upon DNA damage. Within the MRN complex, MRE11 possesses both single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity. MRE11 first endonucleolytically cleaves the 5' strand at DNA DSB ends to prevent non-homologous end joining (NHEJ) and licence HR. It then generates a single-stranded DNA gap via 3' to 5' exonucleolytic degradation, which is required for single-strand invasion and recombination.
Cofactor
Features
Showing features for active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 125 | Proton donor | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | Mre11 complex | |
Cellular Component | site of double-strand break | |
Molecular Function | 3'-5'-DNA exonuclease activity | |
Molecular Function | manganese ion binding | |
Molecular Function | single-stranded DNA endodeoxyribonuclease activity | |
Biological Process | double-strand break repair via homologous recombination | |
Biological Process | double-strand break repair via nonhomologous end joining | |
Biological Process | meiotic DNA double-strand break formation | |
Biological Process | mitochondrial double-strand break repair via homologous recombination | |
Biological Process | mitotic G2 DNA damage checkpoint signaling | |
Biological Process | mitotic intra-S DNA damage checkpoint signaling | |
Biological Process | telomere maintenance |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameDouble-strand break repair protein
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionA0A0L8RDM5
Proteomes
Subcellular Location
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 291-474 | Mre11 DNA-binding | |||
Region | 522-695 | Disordered | |||
Compositional bias | 536-559 | Polar residues | |||
Compositional bias | 560-588 | Basic and acidic residues | |||
Compositional bias | 611-639 | Acidic residues | |||
Sequence similarities
Belongs to the MRE11/RAD32 family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length695
- Mass (Da)78,315
- Last updated2015-11-11 v1
- Checksum1E0670AA227FE04A
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 536-559 | Polar residues | |||
Compositional bias | 560-588 | Basic and acidic residues | |||
Compositional bias | 611-639 | Acidic residues | |||
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
JMCK01000013 EMBL· GenBank· DDBJ | KOG97554.1 EMBL· GenBank· DDBJ | Genomic DNA |