A0A0L0SQ18 · A0A0L0SQ18_ALLM3
- ProteinAcireductone dioxygenase
- GeneADI1
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids186 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes 2 different reactions between oxygen and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4-methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway. Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway.
Catalytic activity
- 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + O2 = 3-(methylsulfanyl)propanoate + CO + formate + 2 H+
Cofactor
Ni2+ (UniProtKB | Rhea| CHEBI:49786 )
Note: Binds either 1 Fe or Ni cation per monomer. Iron-binding promotes an acireductone dioxygenase reaction producing 2-keto-4-methylthiobutyrate, while nickel-binding promotes an acireductone dioxygenase reaction producing 3-(methylsulfanyl)propanoate.
Pathway
Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 5/6.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 91 | Fe2+ (UniProtKB | ChEBI); for iron-dependent acireductone dioxygenase activity | |||
Binding site | 91 | Ni2+ (UniProtKB | ChEBI); for nickel-dependent acireductone dioxygenase activity | |||
Binding site | 93 | Fe2+ (UniProtKB | ChEBI); for iron-dependent acireductone dioxygenase activity | |||
Binding site | 93 | Ni2+ (UniProtKB | ChEBI); for nickel-dependent acireductone dioxygenase activity | |||
Binding site | 97 | Fe2+ (UniProtKB | ChEBI); for iron-dependent acireductone dioxygenase activity | |||
Binding site | 97 | Ni2+ (UniProtKB | ChEBI); for nickel-dependent acireductone dioxygenase activity | |||
Binding site | 136 | Fe2+ (UniProtKB | ChEBI); for iron-dependent acireductone dioxygenase activity | |||
Binding site | 136 | Ni2+ (UniProtKB | ChEBI); for nickel-dependent acireductone dioxygenase activity | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | nucleus | |
Molecular Function | acireductone dioxygenase (Ni2+-requiring) activity | |
Molecular Function | acireductone dioxygenase [iron(II)-requiring] activity | |
Molecular Function | iron ion binding | |
Molecular Function | nickel cation binding | |
Biological Process | L-methionine salvage from methylthioadenosine |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAcireductone dioxygenase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Fungi incertae sedis > Blastocladiomycota > Blastocladiomycetes > Blastocladiales > Blastocladiaceae > Allomyces
Accessions
- Primary accessionA0A0L0SQ18
Proteomes
Organism-specific databases
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length186
- Mass (Da)21,347
- Last updated2015-11-11 v1
- ChecksumBA02BA249F418554
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
GG745345 EMBL· GenBank· DDBJ | KNE64648.1 EMBL· GenBank· DDBJ | Genomic DNA |