A0A0K3AUE4 · SEA2_CAEEL
- ProteinSignal element on autosome protein 2
- Genesea-2
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids1968 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
RNA-binding protein, which regulates the expression of proteins required to control developmental timing of events during the L2 to L3 larval stage switch (PubMed:21471153).
Binds to the 3'UTR of the transcript of the heterochronic protein lin-28 to post-transcriptionally negatively regulate its expression in certain tissue types in the later larval stages (PubMed:21471153).
During larval development, controls the timing of seam cell division and terminal differentiation into adult alae (PubMed:21471153).
In vitro, it can also bind to DNA through its first zinc finger (PubMed:21471153).
May bind directly or indirectly to the promoter of the sex-determining factor xol-1 to activate its transcription (PubMed:23666922).
Its activation of xol-1 transcription controls sex determination and X chromosome dosage compensation to promote male development (PubMed:23666922).
Through the negative regulation of lin-28 transcript, it also has a role in the fox-1-sex-1-mediated determination of sexual fate (PubMed:21471153).
Acts in the intestine to play a role in regulating adult lifespan (PubMed:21471153).
Binds to the 3'UTR of the transcript of the heterochronic protein lin-28 to post-transcriptionally negatively regulate its expression in certain tissue types in the later larval stages (PubMed:21471153).
During larval development, controls the timing of seam cell division and terminal differentiation into adult alae (PubMed:21471153).
In vitro, it can also bind to DNA through its first zinc finger (PubMed:21471153).
May bind directly or indirectly to the promoter of the sex-determining factor xol-1 to activate its transcription (PubMed:23666922).
Its activation of xol-1 transcription controls sex determination and X chromosome dosage compensation to promote male development (PubMed:23666922).
Through the negative regulation of lin-28 transcript, it also has a role in the fox-1-sex-1-mediated determination of sexual fate (PubMed:21471153).
Acts in the intestine to play a role in regulating adult lifespan (PubMed:21471153).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | mediator complex | |
Cellular Component | nucleus | |
Molecular Function | DNA binding | |
Molecular Function | double-stranded RNA binding | |
Molecular Function | metal ion binding | |
Molecular Function | single-stranded RNA binding | |
Molecular Function | transcription coactivator activity | |
Biological Process | cell differentiation | |
Biological Process | determination of adult lifespan | |
Biological Process | negative regulation of gene expression | |
Biological Process | positive regulation of transcription by RNA polymerase II | |
Biological Process | regulation of development, heterochronic | |
Biological Process | regulation of translation | |
Biological Process | response to heat | |
Biological Process | sex differentiation | |
Biological Process | sex-chromosome dosage compensation |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameSignal element on autosome protein 2
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis
Accessions
- Primary accessionA0A0K3AUE4
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
RNAi mediated knockdown at early larval stages and in young adults results in an extended lifespan (PubMed:21471153).
RNAi-mediated knockdown results in delayed developmental defects with an increased number of seam cells in young adults (PubMed:21471153).
RNAi-mediated knockdown suppresses the embryonic lethality of hermaphrodites in the double fox-1 y303 and sex-1 y263 mutant background (PubMed:23666922).
RNAi-mediated knockdown results in delayed developmental defects with an increased number of seam cells in young adults (PubMed:21471153).
RNAi-mediated knockdown suppresses the embryonic lethality of hermaphrodites in the double fox-1 y303 and sex-1 y263 mutant background (PubMed:23666922).
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 415-418 | Abolishes RNA-binding. | |||
Mutagenesis | 415-435 | Does not affect expression pattern. Does not rescue the seam developmental timing defects of the sea-2 b283 mutant. | |||
Mutagenesis | 1398 | In bp283; extended lifespan, and a slow accumulation of dcap-1-positive P-bodies compared to wild-type, indicative of a slower ageing rate. Delays the L2 to L3 larval stage switch. Defective seam cell development due to delayed divisions and failed fusions during the L2 and L3 larval stages. This increases the number of seam cells at later larval stages and delays their terminal differentiation, resulting in discontinuous alae formation. Ectopic expression of the heterochronic protein lin-28 whereby, in contrast to wild-type, lin-28 is expressed in the head, tail and in seam cells of L3 and L4 larvae. Prominent nuclear localization of the transcription factor daf-16 in the intestine resulting in the transcription of daf-16 targets. The seam cell development defects are enhanced in a daf-2 e1370 mutant background. The seam cell development defects are suppressed in a lin-28 n719 mutant background. Partially suppresses the premature alae formation defect in a hbl-1 RNAi mutant background. Suppresses the embryonic lethality of hermaphrodites in the double fox-1 and sex-1 RNAi mutant background. | |||
Mutagenesis | 1670-1673 | Abolishes RNA-binding. | |||
Mutagenesis | 1670-1690 | Does not affect expression pattern. Does not rescue the seam cell developmental timing defects of the sea-2 bp283 mutant. | |||
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000450520 | 1-1968 | Signal element on autosome protein 2 | ||
Proteomic databases
Expression
Tissue specificity
Expressed in seam cells, intestine cells, pharyngeal muscles and nerve ring neurons.
Developmental stage
In embryos, expressed in 20- to 30-cell stage and expression decreases after the 200-cell stage (PubMed:23666922).
Expressed in seam cells, intestine cells, pharyngeal muscles and nerve ring neurons in L3 stage larvae and expression persists into adults (PubMed:21471153).
Expressed in seam cells, intestine cells, pharyngeal muscles and nerve ring neurons in L3 stage larvae and expression persists into adults (PubMed:21471153).
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for compositional bias, region, zinc finger.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 72-109 | Polar residues | |||
Region | 72-252 | Disordered | |||
Compositional bias | 117-208 | Polar residues | |||
Compositional bias | 271-286 | Polar residues | |||
Region | 271-364 | Disordered | |||
Compositional bias | 293-360 | Polar residues | |||
Zinc finger | 413-440 | C2H2-type 1 | |||
Region | 451-499 | Disordered | |||
Compositional bias | 468-499 | Polar residues | |||
Region | 538-601 | Disordered | |||
Compositional bias | 540-560 | Basic and acidic residues | |||
Compositional bias | 571-596 | Basic and acidic residues | |||
Zinc finger | 651-672 | C2H2-type 2; degenerate | |||
Region | 681-712 | Disordered | |||
Compositional bias | 785-810 | Polar residues | |||
Region | 785-854 | Disordered | |||
Compositional bias | 826-854 | Polar residues | |||
Zinc finger | 856-875 | C2H2-type 3; degenerate | |||
Region | 882-906 | Disordered | |||
Compositional bias | 975-1014 | Polar residues | |||
Region | 975-1069 | Disordered | |||
Compositional bias | 1027-1049 | Polar residues | |||
Region | 1083-1227 | Disordered | |||
Compositional bias | 1093-1129 | Polar residues | |||
Compositional bias | 1146-1227 | Polar residues | |||
Region | 1246-1273 | Disordered | |||
Compositional bias | 1251-1268 | Polar residues | |||
Zinc finger | 1274-1297 | C2H2-type 4 | |||
Region | 1333-1478 | Disordered | |||
Compositional bias | 1377-1401 | Basic and acidic residues | |||
Compositional bias | 1410-1445 | Pro residues | |||
Region | 1569-1608 | Disordered | |||
Compositional bias | 1624-1639 | Polar residues | |||
Region | 1624-1671 | Disordered | |||
Zinc finger | 1668-1694 | C2H2-type 5; degenerate | |||
Region | 1769-1822 | Disordered | |||
Compositional bias | 1796-1822 | Polar residues | |||
Zinc finger | 1826-1858 | C2H2-type 6 | |||
Domain
The Zinc finger domains 1 and 5 bind single-stranded and double-stranded RNAs (PubMed:21471153).
The Zinc finger domain 1 binds DNA in vitro (PubMed:21471153).
The Zinc finger domain 1 binds DNA in vitro (PubMed:21471153).
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 19 isoforms produced by Alternative splicing.
A0A0K3AUE4-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Nameg
- Length1,968
- Mass (Da)215,234
- Last updated2015-11-11 v1
- MD5 Checksum5165CA66AADD5C4B7FA98AE7242EAD73
A0A0K3AUE4-2
- Namea
- Differences from canonical
- 1-96: Missing
A0A0K3AUE4-3
- Nameb
- Differences from canonical
- 1-96: Missing
- 687-831: Missing
- 1823-1828: Missing
A0A0K3AUE4-4
- Namec
- Differences from canonical
- 1-96: Missing
- 1816-1825: GAKPSTNQAR → VSGMRQELLQ
- 1826-1968: Missing
A0A0K3AUE4-5
- Named
- Differences from canonical
- 1-338: Missing
- 1816-1825: GAKPSTNQAR → VSGMRQELLQ
- 1826-1968: Missing
A0A0K3AUE4-6
- Namee
- Differences from canonical
- 1-867: Missing
- 1816-1825: GAKPSTNQAR → VSGMRQELLQ
- 1826-1968: Missing
A0A0K3AUE4-7
- Namef
- Differences from canonical
- 1816-1825: GAKPSTNQAR → VSGMRQELLQ
- 1826-1968: Missing
A0A0K3AUE4-8
- Nameh
- Differences from canonical
- 687-831: Missing
- 1816-1825: GAKPSTNQAR → VSGMRQELLQ
- 1826-1968: Missing
A0A0K3AUE4-9
- Namei
- Differences from canonical
- 687-831: Missing
A0A0K3AUE4-10
- Namej
- Differences from canonical
- 1-96: Missing
- 687-831: Missing
- 1816-1825: GAKPSTNQAR → VSGMRQELLQ
- 1826-1968: Missing
A0A0K3AUE4-11
- Namek
- Differences from canonical
- 1-176: Missing
- 1816-1825: GAKPSTNQAR → VSGMRQELLQ
- 1826-1968: Missing
A0A0K3AUE4-12
- Namel
- Differences from canonical
- 1-176: Missing
- 687-831: Missing
- 1816-1825: GAKPSTNQAR → VSGMRQELLQ
- 1826-1968: Missing
A0A0K3AUE4-13
- Namem
- Differences from canonical
- 1-338: Missing
- 687-831: Missing
- 1816-1825: GAKPSTNQAR → VSGMRQELLQ
- 1826-1968: Missing
A0A0K3AUE4-14
- Namen
- Differences from canonical
- 1-176: Missing
A0A0K3AUE4-15
- Nameo
A0A0K3AUE4-16
- Namep
- Differences from canonical
- 1-1852: Missing
A0A0K3AUE4-17
- Nameq
A0A0K3AUE4-18
- Namer
- Differences from canonical
- 1-338: Missing
A0A0K3AUE4-19
- Names
- Differences from canonical
- 1-867: Missing
Features
Showing features for alternative sequence, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Alternative sequence | VSP_060636 | 1-96 | in isoform a, isoform b, isoform c and isoform j | ||
Alternative sequence | VSP_060635 | 1-176 | in isoform k, isoform l, isoform n and isoform o | ||
Alternative sequence | VSP_060634 | 1-338 | in isoform d, isoform m, isoform q and isoform r | ||
Alternative sequence | VSP_060633 | 1-867 | in isoform e and isoform s | ||
Alternative sequence | VSP_060632 | 1-1852 | in isoform p | ||
Compositional bias | 72-109 | Polar residues | |||
Compositional bias | 117-208 | Polar residues | |||
Compositional bias | 271-286 | Polar residues | |||
Compositional bias | 293-360 | Polar residues | |||
Compositional bias | 468-499 | Polar residues | |||
Compositional bias | 540-560 | Basic and acidic residues | |||
Compositional bias | 571-596 | Basic and acidic residues | |||
Alternative sequence | VSP_060637 | 687-831 | in isoform b, isoform h, isoform i, isoform j, isoform l, isoform m, isoform o and isoform q | ||
Compositional bias | 785-810 | Polar residues | |||
Compositional bias | 826-854 | Polar residues | |||
Compositional bias | 975-1014 | Polar residues | |||
Compositional bias | 1027-1049 | Polar residues | |||
Compositional bias | 1093-1129 | Polar residues | |||
Compositional bias | 1146-1227 | Polar residues | |||
Compositional bias | 1251-1268 | Polar residues | |||
Compositional bias | 1377-1401 | Basic and acidic residues | |||
Compositional bias | 1410-1445 | Pro residues | |||
Compositional bias | 1624-1639 | Polar residues | |||
Compositional bias | 1796-1822 | Polar residues | |||
Alternative sequence | VSP_060638 | 1816-1825 | in isoform c, isoform d, isoform e, isoform f, isoform h, isoform j, isoform k, isoform l and isoform m | ||
Alternative sequence | VSP_060639 | 1823-1828 | in isoform b | ||
Alternative sequence | VSP_060640 | 1826-1968 | in isoform c, isoform d, isoform e, isoform f, isoform h, isoform j, isoform k, isoform l and isoform m | ||
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BX284602 EMBL· GenBank· DDBJ | CDG24088.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BX284602 EMBL· GenBank· DDBJ | CDG24089.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BX284602 EMBL· GenBank· DDBJ | CDG24090.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BX284602 EMBL· GenBank· DDBJ | CDG24091.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BX284602 EMBL· GenBank· DDBJ | CDG24092.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BX284602 EMBL· GenBank· DDBJ | CTQ86419.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BX284602 EMBL· GenBank· DDBJ | CTQ86420.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BX284602 EMBL· GenBank· DDBJ | CTQ86421.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BX284602 EMBL· GenBank· DDBJ | CTQ86422.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BX284602 EMBL· GenBank· DDBJ | CTQ86503.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BX284602 EMBL· GenBank· DDBJ | CTQ86504.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BX284602 EMBL· GenBank· DDBJ | CTQ86505.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BX284602 EMBL· GenBank· DDBJ | CTQ86506.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BX284602 EMBL· GenBank· DDBJ | CTQ86507.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BX284602 EMBL· GenBank· DDBJ | CTQ86508.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BX284602 EMBL· GenBank· DDBJ | CTQ86509.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BX284602 EMBL· GenBank· DDBJ | CTQ86510.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BX284602 EMBL· GenBank· DDBJ | CTQ86511.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BX284602 EMBL· GenBank· DDBJ | CTQ86512.1 EMBL· GenBank· DDBJ | Genomic DNA |