A0A0K1RFV2 · A0A0K1RFV2_9GAMC

Function

function

Forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.
Forms a hexadecamer with nsp8 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.
May play a role in the modulation of host cell survival signaling pathway by interacting with host PHB and PHB2 (By similarity).
Indeed, these two proteins play a role in maintaining the functional integrity of the mitochondria and protecting cells from various stresses
Multifunctional protein involved in the transcription and replication of viral RNAs. Contains the proteinases responsible for the cleavages of the polyprotein.
Plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and nsp16 2'-O-methyltransferase activities (By similarity).
Therefore plays an essential role in viral mRNAs cap methylation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site5558
Active site5560
Active site5659
Active site5735
Active site5740
Binding site5798-5804S-adenosyl-L-methionine (UniProtKB | ChEBI)
Active site6213
Active site6228
Active site6268

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componenthost cell endoplasmic reticulum membrane
Cellular Componenthost cell endoplasmic reticulum-Golgi intermediate compartment
Cellular Componenthost cell perinuclear region of cytoplasm
Cellular Componentmembrane
Molecular Function3'-5'-RNA exonuclease activity
Molecular Function5'-3' DNA helicase activity
Molecular FunctionATP binding
Molecular Functioncysteine-type endopeptidase activity
Molecular Functionendonuclease activity
Molecular Functionlyase activity
Molecular FunctionmRNA (nucleoside-2'-O-)-methyltransferase activity
Molecular FunctionmRNA 5'-cap (guanine-N7-)-methyltransferase activity
Molecular Functionomega peptidase activity
Molecular FunctionRNA binding
Molecular FunctionRNA helicase activity
Molecular FunctionRNA-dependent RNA polymerase activity
Molecular Functionzinc ion binding
Biological ProcessDNA-templated transcription
Biological Processinduction by virus of host autophagy
Biological Processproteolysis
Biological Processviral protein processing
Biological Processviral RNA genome replication
Biological Processviral translational frameshifting

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    ORF1ab polyprotein

Organism names

  • Taxonomic identifier
  • Strain
    • GammaCoV/Ck/Italy/I2022/13
  • Taxonomic lineage
    Viruses > Riboviria > Orthornavirae > Pisuviricota > Pisoniviricetes > Nidovirales > Cornidovirineae > Coronaviridae > Orthocoronavirinae > Gammacoronavirus > Igacovirus > Avian coronavirus

Accessions

  • Primary accession
    A0A0K1RFV2

Proteomes

Subcellular Location

Cytoplasm
Endoplasmic reticulum membrane
; Multi-pass membrane protein
Host endoplasmic reticulum membrane
; Multi-pass membrane protein
Membrane
; Multi-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane1748-1764Helical
Transmembrane1845-1872Helical
Transmembrane1884-1909Helical
Transmembrane2282-2300Helical
Transmembrane2559-2581Helical
Transmembrane2587-2608Helical
Transmembrane2615-2631Helical
Transmembrane2643-2676Helical
Transmembrane3098-3118Helical
Transmembrane3124-3141Helical
Transmembrane3153-3176Helical
Transmembrane3196-3213Helical
Transmembrane3220-3239Helical
Transmembrane3259-3282Helical
Transmembrane3294-3321Helical

Keywords

PTM/Processing

Features

Showing features for disulfide bond.

TypeIDPosition(s)Description
Disulfide bond1803↔1809

Keywords

Interaction

Subunit

Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer dsRNA-encircling ring structure (By similarity).
Interacts with ORF6 protein
Homodimer.
Interacts with host PHB and PHB2.
Interacts with papain-like protease and non-structural protein 6.

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain675-780Ubiquitin-like
Domain1004-1180Macro
Domain1176-1228Ubiquitin-like
Domain1237-1498Peptidase C16
Domain1770-18343Ecto
Region1912-2002Y1
Domain1912-2264CoV Nsp3 Y
Region1916-1929ZF1
Region1962-1972ZF2
Region2003-2264CoV-Y
Region2165-2264Y4
Domain2685-2780Nsp4C
Domain2781-3087Peptidase C30
Domain3381-3463RdRp Nsp7 cofactor
Domain3464-3673RdRp Nsp8 cofactor
Domain3674-3784Nsp9 ssRNA-binding
Domain3786-3927ExoN/MTase coactivator
Domain3941-4199NiRAN
Domain4204-4302Nsp12 Interface
Domain4303-4869Nsp12 RNA-dependent RNA polymerase
Domain4549-4711RdRp catalytic
Domain4870-4953CV ZBD
Domain5126-5478+RNA virus helicase C-terminal
Domain5540-5754ExoN
Domain5763-5990N7-MTase
Region5878-5892GpppA-binding
Domain5991-6051Nsp15 N-terminal oligomerization
Domain6052-6167AV-Nsp11N/CoV-Nsp15M
Domain6184-6325NendoU
Domain6328-6627Nidovirus-type SAM-dependent 2'-O-MTase

Sequence similarities

Belongs to the coronaviruses polyprotein 1ab family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    6,630
  • Mass (Da)
    744,544
  • Last updated
    2015-11-11 v1
  • Checksum
    92B3BB35B3B41800

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KP780179
EMBL· GenBank· DDBJ
AKV60428.1
EMBL· GenBank· DDBJ
Genomic RNA

Similar Proteins

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