A0A0K1RFV2 · A0A0K1RFV2_9GAMC
- ProteinORF1ab polyprotein
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids6630 (go to sequence)
- Protein existenceInferred from homology
- Annotation score5/5
Function
function
Forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.
Forms a hexadecamer with nsp8 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.
May play a role in the modulation of host cell survival signaling pathway by interacting with host PHB and PHB2 (By similarity).
Indeed, these two proteins play a role in maintaining the functional integrity of the mitochondria and protecting cells from various stresses
Indeed, these two proteins play a role in maintaining the functional integrity of the mitochondria and protecting cells from various stresses
Multifunctional protein involved in the transcription and replication of viral RNAs. Contains the proteinases responsible for the cleavages of the polyprotein.
Plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and nsp16 2'-O-methyltransferase activities (By similarity).
Therefore plays an essential role in viral mRNAs cap methylation
Therefore plays an essential role in viral mRNAs cap methylation
Catalytic activity
- ATP + H2O = ADP + H+ + phosphate
- ATP + H2O = ADP + H+ + phosphate
- uridylyl-uridylyl-ribonucleotide-RNA = a 3'-end uridylyl-2',3'-cyclophospho-uridine-RNA + a 5'-end dephospho-ribonucleoside-RNA
Cofactor
Protein has several cofactor binding sites:
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 5558 | |||||
Sequence: D | ||||||
Active site | 5560 | |||||
Sequence: E | ||||||
Active site | 5659 | |||||
Sequence: E | ||||||
Active site | 5735 | |||||
Sequence: H | ||||||
Active site | 5740 | |||||
Sequence: D | ||||||
Binding site | 5798-5804 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: DIGNPKG | ||||||
Active site | 6213 | |||||
Sequence: H | ||||||
Active site | 6228 | |||||
Sequence: H | ||||||
Active site | 6268 | |||||
Sequence: K |
GO annotations
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameORF1ab polyprotein
Organism names
- Organism
- Strain
- Taxonomic lineageViruses > Riboviria > Orthornavirae > Pisuviricota > Pisoniviricetes > Nidovirales > Cornidovirineae > Coronaviridae > Orthocoronavirinae > Gammacoronavirus > Igacovirus > Avian coronavirus
Accessions
- Primary accessionA0A0K1RFV2
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Multi-pass membrane protein
Host endoplasmic reticulum membrane ; Multi-pass membrane protein
Membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 1748-1764 | Helical | ||||
Sequence: VLCKVVFTSLLIVWFVY | ||||||
Transmembrane | 1845-1872 | Helical | ||||
Sequence: WLYLVFLILFVKPVAGFVIICYCVKYLV | ||||||
Transmembrane | 1884-1909 | Helical | ||||
Sequence: FLDWFIQTVFTHFNFMGAGFYFWLFY | ||||||
Transmembrane | 2282-2300 | Helical | ||||
Sequence: WLLIFYVLFTACCLGYYHM | ||||||
Transmembrane | 2559-2581 | Helical | ||||
Sequence: IYMQLATMFLILVVVVLIFAMVT | ||||||
Transmembrane | 2587-2608 | Helical | ||||
Sequence: FKTYATIVFTIMLVWFVNAFIL | ||||||
Transmembrane | 2615-2631 | Helical | ||||
Sequence: SVLAVILLVLYCYASLV | ||||||
Transmembrane | 2643-2676 | Helical | ||||
Sequence: WLVFTFGLIVPIWLACCYLAFVLYVYTPLFFWCY | ||||||
Transmembrane | 3098-3118 | Helical | ||||
Sequence: FWSRCVLACFLFVLCAIVLFT | ||||||
Transmembrane | 3124-3141 | Helical | ||||
Sequence: YYVHAAVILLIAVLFISF | ||||||
Transmembrane | 3153-3176 | Helical | ||||
Sequence: FLLPTLITVIIGVCAEVPFIYNTL | ||||||
Transmembrane | 3196-3213 | Helical | ||||
Sequence: MVPWMLLPLVLYTAFKCV | ||||||
Transmembrane | 3220-3239 | Helical | ||||
Sequence: SFNTSLLMLYQFMKLGFVIY | ||||||
Transmembrane | 3259-3282 | Helical | ||||
Sequence: LVHTTVLANVSSNSLIGLFVFKCA | ||||||
Transmembrane | 3294-3321 | Helical | ||||
Sequence: LNNYVLMAVLVNGIGWLCTCYFGLYWWI |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Disulfide bond | 1803↔1809 | |||||
Sequence: CADDFTC |
Keywords
- PTM
Interaction
Subunit
Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer dsRNA-encircling ring structure (By similarity).
Interacts with ORF6 protein
Interacts with ORF6 protein
Homodimer.
Interacts with host PHB and PHB2.
Interacts with papain-like protease and non-structural protein 6.
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 675-780 | Ubiquitin-like | ||||
Sequence: KTVTFGDTTVKEIPPPDVVPIKVSIECCGEPWNTIFKKAYKEPIEVETDLTVEQLLAVIYEKMCDDLKLFPEAPEPPPFENVALVDKNGKDLDCIKSCHLIYRDYE | ||||||
Domain | 1004-1180 | Macro | ||||
Sequence: VKPATCEKPKFLEYTTCVGDLTVVIAKALDEFKEFCIVNAANEYMSHGGGVAKAIADFCGPDFVEYCEDYVKKHGPQQRLVTPSFVKGIQCVNNVVGPRHGDSNLREKLVAAYKNVLVDGVFNYVVPVLSSGIFGVDFKMSIDAMREAFDGCDIRVLLFSLSQDHIDYFDVTCKQKT | ||||||
Domain | 1176-1228 | Ubiquitin-like | ||||
Sequence: CKQKTIYLTEDGVKYRSAIVKPGDSLSQFGQVFAKNKAVFTADDVEDKEILFV | ||||||
Domain | 1237-1498 | Peptidase C16 | ||||
Sequence: DYYHLDAKKYVIYLQTLAQKWDVQYRDNFIVLEWRDGNCWVSSAIVLLQAAKVKFRGFLAEAWAKFLGGDPTDFVAWCYASCNAKVGEFSDANWLLANLAEHFDADYKNALLKKRVSCNCGVKSYELRGLEACIQPVRAPNLLHFKTQYSNCPTCGANSTDEVIEASLPYLLLFATDGPATVDCDENAVGTVVFIGSINSGHCYTQVIGKAFDNLAKDRKFGKKSPYITAMYTRFSLKSENPLPVVKQSQGKTKVVKEDVSN | ||||||
Domain | 1770-1834 | 3Ecto | ||||
Sequence: MFTGISVLDFLFEGSFCSDYNDYGKDSFDVLRYCADDFTCRVCLHDRDSLHLYKHAYSVEQIYKA | ||||||
Region | 1912-2002 | Y1 | ||||
Sequence: YIQVHHILYCKDITCEVCKRVARSNRHEVGVVVGGRKQIVHVYTNSGYKFCKKHNWYCRNCDDYGHQNTFMSPEVAGELSEKLKRHIKPTA | ||||||
Domain | 1912-2264 | CoV Nsp3 Y | ||||
Sequence: YIQVHHILYCKDITCEVCKRVARSNRHEVGVVVGGRKQIVHVYTNSGYKFCKKHNWYCRNCDDYGHQNTFMSPEVAGELSEKLKRHIKPTAHAYHVVEEAYVVDDFVNLKYKAATPGKDSAPIAVKCFSVTDFLKKAVFLKDALKCEQIANDGFIVCNTQSAHSLEEAKNAAIYYAQYLCKPILILDQALYEQLVVEPVSKGVVDKVCNILSNIVSVDTAALNYKAGTLRDTLLSITKDEAAVDMAIFCHNHDVEYTGDGFTNVIPSYGIDTDKLTPRDRGFLINADASVTNLRVKNAPPVVWKFSDLIKLSDSCLKYLISATVKSGSRFFITRSGAKQVFSCHTQKLLVEKK | ||||||
Region | 1916-1929 | ZF1 | ||||
Sequence: HHILYCKDITCEVC | ||||||
Region | 1962-1972 | ZF2 | ||||
Sequence: CKKHNWYCRNC | ||||||
Region | 2003-2264 | CoV-Y | ||||
Sequence: HAYHVVEEAYVVDDFVNLKYKAATPGKDSAPIAVKCFSVTDFLKKAVFLKDALKCEQIANDGFIVCNTQSAHSLEEAKNAAIYYAQYLCKPILILDQALYEQLVVEPVSKGVVDKVCNILSNIVSVDTAALNYKAGTLRDTLLSITKDEAAVDMAIFCHNHDVEYTGDGFTNVIPSYGIDTDKLTPRDRGFLINADASVTNLRVKNAPPVVWKFSDLIKLSDSCLKYLISATVKSGSRFFITRSGAKQVFSCHTQKLLVEKK | ||||||
Region | 2165-2264 | Y4 | ||||
Sequence: VEYTGDGFTNVIPSYGIDTDKLTPRDRGFLINADASVTNLRVKNAPPVVWKFSDLIKLSDSCLKYLISATVKSGSRFFITRSGAKQVFSCHTQKLLVEKK | ||||||
Domain | 2685-2780 | Nsp4C | ||||
Sequence: LYDGNEFVGNYDLAAKSTFVIRGSEFVKLTNEIGDKFEHYLSAYARLKYYSGTGSEQDYLQACRAWLAYALDQYRNSGVEVVYTPPRYSIGVSRLQ | ||||||
Domain | 2781-3087 | Peptidase C30 | ||||
Sequence: AGFKKLVAPSSAVEKCIVSVSYRGNNLNGLWLGDTIYCPRHVLGKFSGDQWSDVLNLANNYEFEVVTQNGVTLNVVSRRLRGAVLILQTAVANAETPKYKFIKANCGDSFTIACSYGGTVVGLYPVTMRSNGTIRASFLAGACGSPGFSIEKGVVNFYYMHHLELPNALHTGTDLMGDFYGGYVDEEVAQRVPPDNLVTNNIVAWLYAAIISVKESSFSLPKWLDSTTVSVEDYNKWAIDNGFTPFSTSTAITKLSAITGVDVCKLLRTIMIKSSQWGSDPILGQYNFEDELTPESVFNQIGGVRLQ | ||||||
Domain | 3381-3463 | RdRp Nsp7 cofactor | ||||
Sequence: SKLSDVKCTTVVLMQLLTKLNVEANSKMHAYLVELHNKILASDDVGECMDNLLGMLITLFCIDSTIDLSEYCDDILKRSTVLQ | ||||||
Domain | 3464-3673 | RdRp Nsp8 cofactor | ||||
Sequence: SVTQEFSHIPSYAEYERAKNLYEKVLADSKNGGVTQQELAAYRKAANIAKSVFDRDLAVQKKLDSMAERAMTTMYKEARVTDRRAKLVSSLHALLFSMLKKIDSEKLNVLFDQASSGVVPLATVPIVCSNKLTLVIPDSETWVKCVEGMHVTYSTVVWNIDTVIDADGTELHPTSIGSGLTYCINGDNIAWPLKVNLTRNGHNKVDAALQ | ||||||
Domain | 3674-3784 | Nsp9 ssRNA-binding | ||||
Sequence: NNELMPHGVKTKACVAGVDQAHCSVESKCYYTNISGNSVVAAITSSNPNLKVASFLNEAGNQIYVDLDPPCKFGMKVGDKVEVVYLYFIKNTRSIVRGMVLGAISNVVVLQ | ||||||
Domain | 3786-3927 | ExoN/MTase coactivator | ||||
Sequence: KGHETEEVDAVGILSLCSFAVDPADTYCKYVAAGSQPLGNCVKMLTVHNGSGFAITSKPSPTPDQDSYGGASVCLYCRAHIAHPGGAGNLDGRCQFKGSFVQIPTTEKDPVGFCLRNKVCTVCQCWIGYGCQCDALRQPKPS | ||||||
Domain | 3941-4199 | NiRAN | ||||
Sequence: YFKRVRGSSEARLIPLSSGCDPDVVKRAFDVCNKESAGMFRNLKRNCARFQEVRDTEDGNLEYCDSFFVVKQTTPSNFEHEKNCYEDLKSNVTADHDFFVFNKNIYNISRQRLTKYTMMDFCYALRHFDPKDCEVLKEILVTYGCIEDCHPKWFEENKDWYDPIENPKYYAMLAKMGPIVRRALLNAIEFGNLMVEKGYVGVVTLDNQDLNGKFYDFGDFQKTAPGAGVPVFDTYYSYMMPIIAMTDALASERYFEYDV | ||||||
Domain | 4204-4302 | Nsp12 Interface | ||||
Sequence: KSYDLLKYDYTEEKQDLFQKYFKYWDQEYHPNCRDCSDDRCLIHCANFNILFSTLIPQTSFGNLCRKVFVDGVPFIATCGYHSKELGVIMNQDNTMSFS | ||||||
Domain | 4303-4869 | Nsp12 RNA-dependent RNA polymerase | ||||
Sequence: KMGLSQLMQFVGDPALLVGTSNNLVDLRTSCFSVCALASGITHQTVKPGHFNKDFYDFAEKAGMFKEGSSIPLKHFFYPQTGNAAINDYDYYRYNRPTMFDIRQLLFCLEVTSKYFECYEGGCIPASQVVVNNLDKSAGYPFNKFGKARLYYEMSLEEQDQLFESTKKNVLPTITQMNLKYAISAKNRARTVAGVSILSTMTNRQFHQKILKSIVNTRNAPVVIGTTKFYGGWDNMLRNLIQGVEDPILMGWDYPKCDRAMPNLLRIAASLVLARKHTNCCTWPERIYRLYNECAQVLSETVLATGGIYVKPGGTSSGDATTAYANSVFNIIQATSANVARLLSVITRDIVYDDIKSLQYELYQQVYRRGNFDPAFVEKFYSYLCKNFSLMILSDDGVVCYNNTLAKQGLVADISGFREILYYQNNVYMADSKCWVEPDLEKGPHEFCSQHTMLVEVDGEPKYLPYPDPSRILGACVFVDEVDKTEPVAVMERYIALAIDAYPLVHHENEEYRKVFFVLLSYIRKLYQELSQSMLMDYSFVMDIDKGSKFWEQEFYENMYRAPTTLQ | ||||||
Domain | 4549-4711 | RdRp catalytic | ||||
Sequence: PILMGWDYPKCDRAMPNLLRIAASLVLARKHTNCCTWPERIYRLYNECAQVLSETVLATGGIYVKPGGTSSGDATTAYANSVFNIIQATSANVARLLSVITRDIVYDDIKSLQYELYQQVYRRGNFDPAFVEKFYSYLCKNFSLMILSDDGVVCYNNTLAKQG | ||||||
Domain | 4870-4953 | CV ZBD | ||||
Sequence: SCGVCVICNSQTILRCGNCIRKPFLCCKCCYDHVMHTDHKNVLSINPYICSQPGCGEADVTKLYLGGMSYFCGNHKPKLSIPLV | ||||||
Domain | 5126-5478 | +RNA virus helicase C-terminal | ||||
Sequence: MVPECFVNNIPLYHLVGKQKRTTVQGPPGSGKSHFAIGLASYFSNARVVFTACSHAAVDALCEKAFKFLKVDDCTRIVPQRTTVDCFSKFKANDTGKKYIFSTINALPEVSCDILLVDEVSMLTNYELSFINGKINYQYVVYVGDPAQLPAPRTLLNGSLSPKDYNVVTNLMVCVKPDIFLAKCYRCPKEIVDTVSTLVYDGKFIANNPESRQCFKVIVNNGNSDVGHESGSAYNTTQLEFVKDFVCRNKEWREATFISPYNAMNQRAYRMLGLNVQTVDSSQGSEYDYVIFCVTADSQHALNINRFNVALTRAKRGILVVMRQRDELYSALKFTELDSETSLQGTGLFKICN | ||||||
Domain | 5540-5754 | ExoN | ||||
Sequence: MFITRDEAIRNVRGWVGFDVEATHACGTNIGTNLPFQVGFSTGADFVVTPEGLVDTSIGNNFEPVNSKAPPGEQFNHLRALFKSAKPWHIIRPRIVQMLADNLCNVSDCVVFVTWCHGLELTTLRYFVKIGKEQVCSCGSRATTFNSHTQAYACWKHCLGFDFVYNPLLVDIQQWGYSGNLQFNHDLHCNVHGHAHVASADAIMTRCLAINNAFC | ||||||
Domain | 5763-5990 | N7-MTase | ||||
Sequence: YPHIANEDEVNSSCRYLQRMYLNACVDALKVNVVYDIGNPKGIKCVRRGDISFRFYDKNPIVPNVKQFEYDYNQHKDKFIDGLCMFWNCNVDCYPDNSLVCRYDTRNLSVFNLPGCNGGSLYVNKHAFHTPKFDRISFRNLKAMPFFFYDSSPCETIQVDGVAQDLVSLATKDCITKCNIGGAVCKKHAQMYAEFVTSYNAAVTAGFTFWVTNNFNPYNLWKSFSALQ | ||||||
Region | 5878-5892 | GpppA-binding | ||||
Sequence: CNGGSLYVNKHAFHT | ||||||
Domain | 5991-6051 | Nsp15 N-terminal oligomerization | ||||
Sequence: SIDNIAYNMYKGGHYDAIAGEMPTIVTGDKVFVIDQGVEKAVFVNQTTLPTSVAFELYAKR | ||||||
Domain | 6052-6167 | AV-Nsp11N/CoV-Nsp15M | ||||
Sequence: NIRTLPNNRILKGLGVDVTNGFVIWDYANQTPLYRNTVKVCAYTDIEPNGLIVLYDDRYGDYQSFLAADNAVLVSTQCYKRYSYVEIPSNMLVQNGMPLKDGANLYVYKRVNGAFV | ||||||
Domain | 6184-6325 | NendoU | ||||
Sequence: EPRSDVERDFLDMAEDDFVEKYGKDLGLQHILYGEVAKPQLGGLHTVIGMYRLLRANKLNAKSVTNSDSDVMQNYFVLADNGSYKQVCTVVDLLLDDFLELLRNILNEYGTNKSKVVTVSIDYHSINFMTWFEDGSIKTCYP | ||||||
Domain | 6328-6627 | Nidovirus-type SAM-dependent 2'-O-MTase | ||||
Sequence: QSAWTCGYNMPELYKVQNCVMEPCNIPNYGVGITLPSGIMMNVAKYTQLCQYLSKTTMCVPHNMRVMHFGAGSDKGVAPGSTVLKQWLPEGTLLVDNDIVDYVSDAHVSVLSDCNKYKTEHKFDLVISDMYTDNDSKRKHEGVIANNGNDDVFIYLSDFLRNNLALGGSFAVKVTETSWHESLYDIAQDCAWWTMFCTAVNASSSEAFLVGVNYLGASEKLKVSGKTLHANYIFWRNCNYLQTSAYSVFDVAKFGLKLKATPVVNLKTEQKTDLVFNLLKNGKLLIRDVGEVTVYSDSFV |
Sequence similarities
Belongs to the coronaviruses polyprotein 1ab family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length6,630
- Mass (Da)744,544
- Last updated2015-11-11 v1
- Checksum92B3BB35B3B41800
Keywords
- Coding sequence diversity