A0A0J8RIP0 · A0A0J8RIP0_COCIT

  • Protein
    NADPH-dependent diflavin oxidoreductase 1
  • Gene
    TAH18
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

NADPH-dependent reductase which is a central component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Transfers electrons from NADPH via its FAD and FMN prosthetic groups to the [2Fe-2S] cluster of DRE2, another key component of the CIA machinery. In turn, this reduced cluster provides electrons for assembly of cytosolic iron-sulfur cluster proteins. Positively controls H2O2-induced cell death.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
FAD (UniProtKB | Rhea| CHEBI:57692 )

FMN (UniProtKB | Rhea| CHEBI:58210 )

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site18-23FMN (UniProtKB | ChEBI)
Binding site65-68FMN (UniProtKB | ChEBI)
Binding site103-112FMN (UniProtKB | ChEBI)
Binding site138FMN (UniProtKB | ChEBI)
Binding site385FAD (UniProtKB | ChEBI)
Binding site415-418FAD (UniProtKB | ChEBI)
Binding site454-457FAD (UniProtKB | ChEBI)
Binding site496NADP+ (UniProtKB | ChEBI)
Binding site565-566NADP+ (UniProtKB | ChEBI)
Binding site571-575NADP+ (UniProtKB | ChEBI)
Binding site645FAD (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentmitochondrion
Molecular Functionflavin adenine dinucleotide binding
Molecular FunctionFMN binding
Molecular FunctionNADP binding
Molecular FunctionNADPH-hemoprotein reductase activity
Biological Processiron-sulfur cluster assembly

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    NADPH-dependent diflavin oxidoreductase 1
  • EC number
  • Alternative names
    • NADPH-dependent FMN and FAD-containing oxidoreductase

Gene names

    • Name
      TAH18
    • ORF names
      CIHG_02496

Organism names

  • Taxonomic identifier
  • Strain
    • H538.4
  • Taxonomic lineage
    Eukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Eurotiomycetes > Eurotiomycetidae > Onygenales > Onygenaceae > Coccidioides

Accessions

  • Primary accession
    A0A0J8RIP0

Proteomes

Organism-specific databases

Subcellular Location

Cytoplasm
Mitochondrion
Note: Relocalizes to mitochondria after H2O2 exposure.

Keywords

Interaction

Subunit

Interacts with DRE2; as part of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, region, compositional bias.

TypeIDPosition(s)Description
Domain12-156Flavodoxin-like
Region187-211Disordered
Compositional bias192-211Polar residues
Domain233-481FAD-binding FR-type

Sequence similarities

Belongs to the NADPH-dependent diflavin oxidoreductase NDOR1 family.
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
In the N-terminal section; belongs to the flavodoxin family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    645
  • Mass (Da)
    72,784
  • Last updated
    2015-10-14 v1
  • Checksum
    D26E991DEABA8A98
MASSKNGVPRTALVVYASETGNSQEIAEELGRLTERLHFETHVSELDAIEAESLNGHSLTIFAVSTTGQGDVPANGKTFWRSLLLKRLLPTYLQHVNFALFGLGDSSYPKFNWAARKMHKRLLQLGANEIFPSGEADEQHPEGIDGTFVPWAQSLKKFLLDKFPLKPGQHPIPDDVRLPPKWALAPWGQGTGKEHQMTNGNSSQPEKNNGTGKEQIVQYPALQPRDHDTRPIPNSITATLIDNIRVTPESHWQDVRHLTLTVPESMQYIPGDILHITPKNFAKDVNTLLSLMGWEADADIPLCFTPASKSSLPSSSPPISFLQNSPGFTLRELLTNYLDIMAIPRRSFFSQISHFTDDTMQKERLLEFTNPEYIDEYYDYATRSRRSILEVLYEFDTVKVPWQQVCNVFPILRGRQFSIASGGRLKKTVEGKTKFELLVAIVKYQTVIKKIREGVCTRYLAVLQPGSTMKVQLHRGGLSPSVKQLLEPSVVIGPGTGVAPIRSLLWEKAALVEAYRNKHGPNVPSPVGPVILLYGGRNRAADFFFQEEWNKLKETLDLTVFTAFSRDQKHKFYVQDAIRQNKDAFFRVLHDMQGAVFICGSSGRMPQAVREALIETFETRGSSREEAEKYLIDMERVGRYKQETW

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias192-211Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
DS016986
EMBL· GenBank· DDBJ
KMU84712.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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