A0A0J1G8B4 · A0A0J1G8B4_9BURK

  • Protein
    tRNA-specific 2-thiouridylase MnmA
  • Gene
    mnmA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s2U34.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Features

Showing features for binding site, active site, site.

136950100150200250300350
TypeIDPosition(s)Description
Binding site9-16ATP (UniProtKB | ChEBI)
Binding site35ATP (UniProtKB | ChEBI)
Active site100Nucleophile
Binding site124ATP (UniProtKB | ChEBI)
Site125Interaction with tRNA
Active site198Cysteine persulfide intermediate
Site347Interaction with tRNA

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular FunctiontRNA binding
Molecular FunctiontRNA-uridine 2-sulfurtransferase activity
Biological ProcesstRNA wobble position uridine thiolation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA-specific 2-thiouridylase MnmA
  • EC number

Gene names

    • Name
      mnmA
    • ORF names
      AB595_20315

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • WF1
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Betaproteobacteria > Burkholderiales > Oxalobacteraceae > Telluria group > Massilia

Accessions

  • Primary accession
    A0A0J1G8B4

Proteomes

Subcellular Location

Keywords

PTM/Processing

Keywords

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain6-50Rhodanese
Region95-97Interaction with target base in tRNA
Region148-150Interaction with tRNA
Region314-315Interaction with tRNA

Sequence similarities

Belongs to the MnmA/TRMU family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    369
  • Mass (Da)
    40,567
  • Last updated
    2015-10-14 v1
  • Checksum
    541A501862A47D04
MTKKKVVIGMSGGVDSSVAAWMLKEQGYEVVGLFMKNWEDDDDSEYCSTRQDWIDAASVADVVGVDIEAVNFAAEYKDRVFAEFLREYQAGRTPNPDVLCNAEIKFKAFLDHAMKLGADLIATGHYARVRHNQASDRFELLKALDSTKDQSYFLHRLNQAQLSKALFPLGEIAKTEVRKIAEKLNLPNAAKKDSTGICFIGERPFREFLGRYLQHKPGPIKLDNGQTVGEHVGLSFYTLGQRKGIGIGGLKSHKNADGTSEPWFVARKDIANNTLYIVQGHDHPWLLSAELGAGQASWVAGEPPAPRSLSAKTRYRQADVACTVAPEGQDRFLLRFDEPQWAVTPGQSAVLYDGDVCLGGGIIDSASAA

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
LELH02000030
EMBL· GenBank· DDBJ
KLU35141.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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