A0A0J1G8B4 · A0A0J1G8B4_9BURK
- ProteintRNA-specific 2-thiouridylase MnmA
- GenemnmA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids369 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s2U34.
Catalytic activity
- AH2 + ATP + S-sulfanyl-L-cysteinyl-[protein] + uridine34 in tRNA = 2-thiouridine34 in tRNA + A + AMP + diphosphate + H+ + L-cysteinyl-[protein]
CHEBI:17499 + CHEBI:30616 + RHEA-COMP:11726 + RHEA-COMP:11727 CHEBI:65315 Position: 34= RHEA-COMP:11728 CHEBI:87170 Position: 34+ CHEBI:13193 + CHEBI:456215 + CHEBI:33019 + CHEBI:15378 + RHEA-COMP:10131
Features
Showing features for binding site, active site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 9-16 | ATP (UniProtKB | ChEBI) | ||||
Sequence: GMSGGVDS | ||||||
Binding site | 35 | ATP (UniProtKB | ChEBI) | ||||
Sequence: M | ||||||
Active site | 100 | Nucleophile | ||||
Sequence: C | ||||||
Binding site | 124 | ATP (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Site | 125 | Interaction with tRNA | ||||
Sequence: H | ||||||
Active site | 198 | Cysteine persulfide intermediate | ||||
Sequence: C | ||||||
Site | 347 | Interaction with tRNA | ||||
Sequence: Q |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | tRNA binding | |
Molecular Function | tRNA-uridine 2-sulfurtransferase activity | |
Biological Process | tRNA wobble position uridine thiolation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA-specific 2-thiouridylase MnmA
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Betaproteobacteria > Burkholderiales > Oxalobacteraceae > Telluria group > Massilia
Accessions
- Primary accessionA0A0J1G8B4
Proteomes
Subcellular Location
PTM/Processing
Keywords
- PTM
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 6-50 | Rhodanese | ||||
Sequence: VVIGMSGGVDSSVAAWMLKEQGYEVVGLFMKNWEDDDDSEYCSTR | ||||||
Region | 95-97 | Interaction with target base in tRNA | ||||
Sequence: NPD | ||||||
Region | 148-150 | Interaction with tRNA | ||||
Sequence: KDQ | ||||||
Region | 314-315 | Interaction with tRNA | ||||
Sequence: RY |
Sequence similarities
Belongs to the MnmA/TRMU family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length369
- Mass (Da)40,567
- Last updated2015-10-14 v1
- Checksum541A501862A47D04
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
LELH02000030 EMBL· GenBank· DDBJ | KLU35141.1 EMBL· GenBank· DDBJ | Genomic DNA |