A0A0H3MZ55 · A0A0H3MZ55_CLODC
- ProteintRNA N6-adenosine threonylcarbamoyltransferase
- Genegcp
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids338 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.
Catalytic activity
- L-threonylcarbamoyladenylate + adenosine37 in tRNA = N6-L-threonylcarbamoyladenosine37 in tRNA + AMP + H+
Cofactor
Note: Binds 1 Fe2+ ion per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 114 | Fe cation (UniProtKB | ChEBI) | |||
Binding site | 118 | Fe cation (UniProtKB | ChEBI) | |||
Binding site | 137-141 | substrate | |||
Binding site | 170 | substrate | |||
Binding site | 183 | substrate | |||
Binding site | 187 | substrate | |||
Binding site | 277 | substrate | |||
Binding site | 305 | Fe cation (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | iron ion binding | |
Molecular Function | N(6)-L-threonylcarbamoyladenine synthase activity | |
Biological Process | tRNA threonylcarbamoyladenosine modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA N6-adenosine threonylcarbamoyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Clostridia > Peptostreptococcales > Peptostreptococcaceae > Clostridioides
Accessions
- Primary accessionA0A0H3MZ55
Proteomes
Subcellular Location
Structure
Sequence
- Sequence statusComplete
- Length338
- Mass (Da)36,506
- Last updated2015-09-16 v1
- MD5 Checksum4DE0D9A05E9ED2DB787F7EA3304D7AF5
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
FN538970 EMBL· GenBank· DDBJ | CBA60342.1 EMBL· GenBank· DDBJ | Genomic DNA |