A0A0H3H456 · DHAM_KLEM8

Function

function

Component of the dihydroxyacetone kinase complex, which is responsible for the phosphoenolpyruvate (PEP)-dependent phosphorylation of dihydroxyacetone. DhaM serves as the phosphoryl donor. Is phosphorylated by phosphoenolpyruvate in an EI- and HPr-dependent reaction, and a phosphorelay system on histidine residues finally leads to phosphoryl transfer to DhaL and dihydroxyacetone.

Miscellaneous

Unlike the carbohydrate-specific transporters of the PTS, the complex DhaKLM has no transport activity.

Caution

Was reported to be a protein deacetylase that removes acetyl groups on specific lysine residues in target proteins (PubMed:26716769).
However, later experiments demonstrate that the protein ortholog in E.coli does not have any protein deacetylase activity; the discrepancy observed seems to be due to contaminants having proteolytic activity (PubMed:29939131).

Catalytic activity

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site9Tele-phosphohistidine intermediate
Active site170Pros-phosphohistidine intermediate
Active site432Tele-phosphohistidine intermediate

GO annotations

AspectTerm
Cellular Componentmembrane
Molecular Functionphosphoenolpyruvate-glycerone phosphotransferase activity
Biological Processglycerol catabolic process
Biological Processphosphoenolpyruvate-dependent sugar phosphotransferase system

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    PEP-dependent dihydroxyacetone kinase, phosphoryl donor subunit DhaM
  • EC number
  • Alternative names
    • Dihydroxyacetone kinase subunit M

Gene names

    • Name
      dhaM
    • Ordered locus names
      KOX_03180

Organism names

Accessions

  • Primary accession
    A0A0H3H456

Proteomes

Subcellular Location

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004358891-472PEP-dependent dihydroxyacetone kinase, phosphoryl donor subunit DhaM

Interaction

Subunit

Homodimer. The dihydroxyacetone kinase complex is composed of a homodimer of DhaM, a homodimer of DhaK and the subunit DhaL.

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain1-135PTS EIIA type-4
Domain156-243HPr
Region266-472PTS EI-like, N-terminal part

Domain

Consists of three domains. The N-terminal dimerization domain has the same fold as the IIA domain of the mannose transporter of the bacterial phosphoenolpyruvate:sugar phosphotransferase system (PTS). The middle domain is similar to HPr and the C-terminus is similar to the N-terminal domain of enzyme I (EI) of the PTS. The IIA domain of DhaM (via phospho-His-9), instead of ATP, is the phosphoryl donor to dihydroxyacetone (Dha). The phosphoryl flow likely involves HPr ('His-15') -> DhaM (His-432 -> His-170 -> His-9) -> DhaL-ADP -> Dha. The HPr-like domain of DhaM cannot efficiently substitute for the general carrier protein HPr.

Sequence similarities

Belongs to the PEP-utilizing enzyme family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    472
  • Mass (Da)
    50,197
  • Last updated
    2015-09-16 v1
  • Checksum
    E67D4B95245DE206
MVNLVIVSHSARLGEGVGELARQMLMNDGCKLAIAAGIDDPASPIGTDPIKVMEAIESVADADHILVMMDIGSALLSAETALDLLDPAIAAKVRLCAAPLVEGTLAATVSAAAGAGIDKVIEDAMNALEAKRVQLGLPSQPQHASLTAAPVDDRDARSVSVVIQNHNGLHVRPASKLVAALAGFNADLVLEKGGKCVTPDSLNQIALLQVRRNDTLRLLARGPDADAALAAFQALAAENFGEPTEAAPARRPASADRVEGKVVLYPQPQDRISRETSAAIGQQQLRLKRAIDRTLEDLSALTTLAEATFSADIAAIFSGHHTLLDDPDLYAAACDIIRDEQCSAAWAWQQVLSDLSQQYRHLDDAYLQARYIDIEDILHRTLRHLNERNEALPQFSAPSILVADDIFPSTVLQLNAEQVKGICLQAGSELSHGAIIARQAGIAMLCQQSDALTLQDGENVILDIPGKRVIRG

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP003218
EMBL· GenBank· DDBJ
AEX02377.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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