A0A0H3H456 · DHAM_KLEM8
- ProteinPEP-dependent dihydroxyacetone kinase, phosphoryl donor subunit DhaM
- GenedhaM
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids472 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Component of the dihydroxyacetone kinase complex, which is responsible for the phosphoenolpyruvate (PEP)-dependent phosphorylation of dihydroxyacetone. DhaM serves as the phosphoryl donor. Is phosphorylated by phosphoenolpyruvate in an EI- and HPr-dependent reaction, and a phosphorelay system on histidine residues finally leads to phosphoryl transfer to DhaL and dihydroxyacetone.
Miscellaneous
Unlike the carbohydrate-specific transporters of the PTS, the complex DhaKLM has no transport activity.
Catalytic activity
- dihydroxyacetone + phosphoenolpyruvate = dihydroxyacetone phosphate + pyruvate
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 9 | Tele-phosphohistidine intermediate | ||||
Sequence: H | ||||||
Active site | 170 | Pros-phosphohistidine intermediate | ||||
Sequence: H | ||||||
Active site | 432 | Tele-phosphohistidine intermediate | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | membrane | |
Molecular Function | phosphoenolpyruvate-glycerone phosphotransferase activity | |
Biological Process | glycerol catabolic process | |
Biological Process | phosphoenolpyruvate-dependent sugar phosphotransferase system |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePEP-dependent dihydroxyacetone kinase, phosphoryl donor subunit DhaM
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Klebsiella/Raoultella group > Klebsiella
Accessions
- Primary accessionA0A0H3H456
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000435889 | 1-472 | PEP-dependent dihydroxyacetone kinase, phosphoryl donor subunit DhaM | |||
Sequence: MVNLVIVSHSARLGEGVGELARQMLMNDGCKLAIAAGIDDPASPIGTDPIKVMEAIESVADADHILVMMDIGSALLSAETALDLLDPAIAAKVRLCAAPLVEGTLAATVSAAAGAGIDKVIEDAMNALEAKRVQLGLPSQPQHASLTAAPVDDRDARSVSVVIQNHNGLHVRPASKLVAALAGFNADLVLEKGGKCVTPDSLNQIALLQVRRNDTLRLLARGPDADAALAAFQALAAENFGEPTEAAPARRPASADRVEGKVVLYPQPQDRISRETSAAIGQQQLRLKRAIDRTLEDLSALTTLAEATFSADIAAIFSGHHTLLDDPDLYAAACDIIRDEQCSAAWAWQQVLSDLSQQYRHLDDAYLQARYIDIEDILHRTLRHLNERNEALPQFSAPSILVADDIFPSTVLQLNAEQVKGICLQAGSELSHGAIIARQAGIAMLCQQSDALTLQDGENVILDIPGKRVIRG |
Interaction
Subunit
Homodimer. The dihydroxyacetone kinase complex is composed of a homodimer of DhaM, a homodimer of DhaK and the subunit DhaL.
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 1-135 | PTS EIIA type-4 | ||||
Sequence: MVNLVIVSHSARLGEGVGELARQMLMNDGCKLAIAAGIDDPASPIGTDPIKVMEAIESVADADHILVMMDIGSALLSAETALDLLDPAIAAKVRLCAAPLVEGTLAATVSAAAGAGIDKVIEDAMNALEAKRVQL | ||||||
Domain | 156-243 | HPr | ||||
Sequence: ARSVSVVIQNHNGLHVRPASKLVAALAGFNADLVLEKGGKCVTPDSLNQIALLQVRRNDTLRLLARGPDADAALAAFQALAAENFGEP | ||||||
Region | 266-472 | PTS EI-like, N-terminal part | ||||
Sequence: PQPQDRISRETSAAIGQQQLRLKRAIDRTLEDLSALTTLAEATFSADIAAIFSGHHTLLDDPDLYAAACDIIRDEQCSAAWAWQQVLSDLSQQYRHLDDAYLQARYIDIEDILHRTLRHLNERNEALPQFSAPSILVADDIFPSTVLQLNAEQVKGICLQAGSELSHGAIIARQAGIAMLCQQSDALTLQDGENVILDIPGKRVIRG |
Domain
Consists of three domains. The N-terminal dimerization domain has the same fold as the IIA domain of the mannose transporter of the bacterial phosphoenolpyruvate:sugar phosphotransferase system (PTS). The middle domain is similar to HPr and the C-terminus is similar to the N-terminal domain of enzyme I (EI) of the PTS. The IIA domain of DhaM (via phospho-His-9), instead of ATP, is the phosphoryl donor to dihydroxyacetone (Dha). The phosphoryl flow likely involves HPr ('His-15') -> DhaM (His-432 -> His-170 -> His-9) -> DhaL-ADP -> Dha. The HPr-like domain of DhaM cannot efficiently substitute for the general carrier protein HPr.
Sequence similarities
Belongs to the PEP-utilizing enzyme family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length472
- Mass (Da)50,197
- Last updated2015-09-16 v1
- ChecksumE67D4B95245DE206
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP003218 EMBL· GenBank· DDBJ | AEX02377.1 EMBL· GenBank· DDBJ | Genomic DNA |