A0A0H3F6Z6 · A0A0H3F6Z6_RAHSY

  • Protein
    Dual-specificity RNA methyltransferase RlmN
  • Gene
    rlmN
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs. m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity.

Miscellaneous

Reaction proceeds by a ping-pong mechanism involving intermediate methylation of a conserved cysteine residue.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site119Proton acceptor
Binding site139[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site143[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site146[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site193-194S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site225S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site247-249S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site326S-adenosyl-L-methionine (UniProtKB | ChEBI)
Active site369S-methylcysteine intermediate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionmetal ion binding
Molecular FunctionrRNA (adenine(2503)-C2-)-methyltransferase activity
Molecular FunctionrRNA binding
Molecular FunctiontRNA (adenine(37)-C2)-methyltransferase activity
Molecular FunctiontRNA binding
Biological ProcessrRNA base methylation
Biological ProcesstRNA methylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Dual-specificity RNA methyltransferase RlmN
  • EC number
  • Alternative names
    • 23S rRNA (adenine(2503)-C(2))-methyltransferase
    • 23S rRNA m2A2503 methyltransferase
    • Ribosomal RNA large subunit methyltransferase N
    • tRNA (adenine(37)-C(2))-methyltransferase
    • tRNA m2A37 methyltransferase

Gene names

    • Name
      rlmN
    • Ordered locus names
      Rahaq_1040

Organism names

  • Taxonomic identifier
  • Strain
    • Y9602
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Yersiniaceae > Rahnella

Accessions

  • Primary accession
    A0A0H3F6Z6

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for disulfide bond.

TypeIDPosition(s)Description
Disulfide bond132↔369(transient)

Keywords

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain125-364Radical SAM core

Sequence similarities

Belongs to the radical SAM superfamily. RlmN family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    399
  • Mass (Da)
    44,138
  • Last updated
    2015-09-16 v1
  • Checksum
    D89F14399B25C83A
MLESNTSEPILSENNAVSAEAVNTVVPAAAKINLLDLNRKQMREFFINMGEKPFRADQVMKWMYHYCSDDFEQMTDINKALREKLARVAEIRAPEVASEQRSTDGTIKWAIQVGGQLVETVYIPDGDRATLCVSSQVGCALECTFCSTAQQGFNRNLRVSEIIGQVWRAAKIIGAAKVAGTRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDMIDVALAISLHAPNDAIRDEIVPINRKYNIDTFLAAVERYISKSNANQGRVTIEYVMLDHINDGTEHAHELAERLKNTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVLMDYGFTVIVRKTRGDDIDAACGQLAGDVIDRTKRTLKKKMAGEQQISVKAV

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP002505
EMBL· GenBank· DDBJ
ADW72663.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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