A0A0H3EM34 · A0A0H3EM34_ECO8N
- ProteintRNA(Met) cytidine acetyltransferase TmcA
- GenetmcA
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids671 (go to sequence)
- Protein existenceInferred from homology
- Annotation score2/5
Function
function
Catalyzes the formation of N4-acetylcytidine (ac4C) at the wobble position of tRNA(Met), by using acetyl-CoA as an acetyl donor and ATP (or GTP).
Catalytic activity
- cytidine34 in elongator tRNA(Met) + acetyl-CoA + ATP + H2O = N4-acetylcytidine34 in elongator tRNA(Met) + ADP + phosphate + CoA + H+
RHEA-COMP:10693 CHEBI:82748 Position: 34+ CHEBI:57288 + CHEBI:30616 + CHEBI:15377 = RHEA-COMP:10694 CHEBI:74900 Position: 34+ CHEBI:456216 + CHEBI:43474 + CHEBI:57287 + CHEBI:15378
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 174-175 | ATP (UniProtKB | ChEBI) | |||
Binding site | 180 | ATP (UniProtKB | ChEBI) | |||
Binding site | 202-211 | ATP (UniProtKB | ChEBI) | |||
Binding site | 319 | ATP (UniProtKB | ChEBI) | |||
Binding site | 461-463 | acetyl-CoA (UniProtKB | ChEBI) | |||
Binding site | 468-474 | acetyl-CoA (UniProtKB | ChEBI) | |||
Binding site | 499 | acetyl-CoA (UniProtKB | ChEBI) | |||
Binding site | 506 | acetyl-CoA (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | hydrolase activity | |
Molecular Function | tRNA binding | |
Molecular Function | tRNA N-acetyltransferase activity | |
Biological Process | tRNA acetylation | |
Biological Process | tRNA wobble cytosine modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA(Met) cytidine acetyltransferase TmcA
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionA0A0H3EM34
Proteomes
Subcellular Location
Structure
Sequence
- Sequence statusComplete
- Length671
- Mass (Da)74,852
- Last updated2015-09-16 v1
- MD5 Checksum94FE40A6D73292899E59FE5C65AAD355
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP001855 EMBL· GenBank· DDBJ | ADR27886.1 EMBL· GenBank· DDBJ | Genomic DNA |