A0A0H3DI45 · A0A0H3DI45_AMYMU
- Protein3'-phosphate/5'-hydroxy nucleic acid ligase
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids396 (go to sequence)
- Protein existencePredicted
- Annotation score2/5
Function
Catalytic activity
- a 3'-end 3'-phospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP = a ribonucleotidyl-ribonucleotide-RNA + GMP + diphosphate
Cofactor
Note: Binds 2 manganese ions per subunit.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 73 | Mn2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 159-163 | GMP (UniProtKB | ChEBI) | |||
Binding site | 160 | Mn2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 181 | Mn2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 269 | Mn2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 269-270 | GMP (UniProtKB | ChEBI) | |||
Binding site | 301-304 | GMP (UniProtKB | ChEBI) | |||
Binding site | 308 | GMP (UniProtKB | ChEBI) | |||
Active site | 325 | GMP-histidine intermediate | |||
Binding site | 325-328 | GMP (UniProtKB | ChEBI) | |||
Binding site | 395 | GMP (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | DNA ligase activity | |
Molecular Function | GTP binding | |
Molecular Function | manganese ion binding | |
Molecular Function | RNA ligase (GTP) activity | |
Biological Process | DNA repair | |
Biological Process | RNA processing | |
Biological Process | RNA repair |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name3'-phosphate/5'-hydroxy nucleic acid ligase
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Pseudonocardiales > Pseudonocardiaceae > Amycolatopsis
Accessions
- Primary accessionA0A0H3DI45
Proteomes
Structure
Sequence
- Sequence statusComplete
- Length396
- Mass (Da)42,404
- Last updated2015-09-16 v1
- MD5 Checksum27B27F6BEAB1A7336B3362BBA5FE9150
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP002000 EMBL· GenBank· DDBJ | ADJ50570.1 EMBL· GenBank· DDBJ | Genomic DNA |