A0A0H3AS38 · A0A0H3AS38_BRUO2

Function

function

APS kinase catalyzes the synthesis of activated sulfate.
Catalyzes the synthesis of activated sulfate.
Proposed to provide activated sulfate for transfer to Nod factor. ATP sulfurylase may be the GTPase, regulating ATP sulfurylase activity.
With CysD forms the ATP sulfurylase (ATPS) that catalyzes the adenylation of sulfate producing adenosine 5'-phosphosulfate (APS) and diphosphate, the first enzymatic step in sulfur assimilation pathway. APS synthesis involves the formation of a high-energy phosphoric-sulfuric acid anhydride bond driven by GTP hydrolysis by CysN coupled to ATP hydrolysis by CysD.

Catalytic activity

Pathway

Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3.
Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 2/3.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site33-40GTP (UniProtKB | ChEBI)
Binding site112-116GTP (UniProtKB | ChEBI)
Binding site167-170GTP (UniProtKB | ChEBI)
Binding site469-476ATP (UniProtKB | ChEBI)
Active site543Phosphoserine intermediate

GO annotations

AspectTerm
Molecular Functionadenylylsulfate kinase activity
Molecular FunctionATP binding
Molecular FunctionGTP binding
Molecular FunctionGTPase activity
Molecular Functionsulfate adenylyltransferase (ATP) activity
Biological Processhydrogen sulfide biosynthetic process
Biological Processphosphorylation
Biological Processsulfate assimilation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Multifunctional fusion protein

Including 2 domains:

  • Recommended name
    Sulfate adenylyltransferase subunit 1
  • EC number
  • Alternative names
    • ATP-sulfurylase large subunit
    • Sulfate adenylate transferase
      (SAT
      )
  • Recommended name
    Adenylyl-sulfate kinase
  • EC number
  • Alternative names
    • APS kinase
    • ATP adenosine-5'-phosphosulfate 3'-phosphotransferase
    • Adenosine-5'-phosphosulfate kinase

Gene names

    • Name
      cysNC
    • Synonyms
      cysC
      , cysN
    • Ordered locus names
      BOV_0186

Organism names

Accessions

  • Primary accession
    A0A0H3AS38

Proteomes

PTM/Processing

Keywords

Interaction

Subunit

Heterodimer composed of CysD, the smaller subunit, and CysN.
Sulfate-activating enzymes, NodP and NodQ, may be physically associated.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain24-240Tr-type G

Sequence similarities

Belongs to the APS kinase family.
In the C-terminal section; belongs to the APS kinase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    644
  • Mass (Da)
    71,085
  • Last updated
    2015-09-16 v1
  • Checksum
    1EA78A7EDF7F7CD8
MNVVMKPSQKNTAVADFLADQEHKSLLRFLTCGSVDDGKSTLIGRLLYDTKLIFEDQLASLKNDSRKFGTTDDDFDFALLVDGLEAEREQGITIDVAYRFFSTPRRKFIVADTPGHAQYTRNMATGASTADLAVVLVDARQGVLTQTRRHSFIAAALGILHIVLAVNKIDLVDFSQDRFDGIVADYLSFAQDLGFTSIQPIPLSARFGDNVTASSPRIGWYEGPHLLEHLETVRIDTEAAAKPFRFPVQYVNRPNLDFRGFSGTVASGSIHVGDPVVVAKSGKQSRVKRIATYDGDLQRASEGQAVTLVLEDEIEVSRGNMLAGVELRPEVADRFAADIVWFGEDPLLPGRSYLLRTETDQTPVTVNAIRHRTDVNTFIQEETARLDLNEIGRCHLQTVDPIAFDPYGENRATGAFVLIDRLTNATVGAGMIDAALRQATNVHLQAFDLNKQARAAQKFQKPAVLWFTGLSASGKSTIANRLEQRLHALGKHTYLLDGDNVRHGLNSDLGFSDADRVENIRRVGEVARLMADAGLITLVSFISPFREERDQVRARLPEGEFIEIFIDTPIEECMARDPKGLYAQALRGEIKAFTGIDSPYEPPVAPELRLNTAGRTVDELIAEVENYLAERGVIGSYGSDSWSI

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000708
EMBL· GenBank· DDBJ
ABQ61198.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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