A0A0H2ZQT7 · WALJ_STRP2
- ProteinExodeoxyribonuclease WalJ
- GenewalJ
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids269 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
5'->3' double-stranded DNA exonuclease (By similarity).
May be involved in the WalK/WalR signal transduction pathway (By similarity).
Required for accurate coordination of cell division with DNA replication (By similarity).
May play a role in cell wall metabolism (PubMed:21169496).
May be involved in the WalK/WalR signal transduction pathway (By similarity).
Required for accurate coordination of cell division with DNA replication (By similarity).
May play a role in cell wall metabolism (PubMed:21169496).
Miscellaneous
Part of a walR-walK-walJ operon.
Present in about 1200 monomers per cell in cultures growing exponentially.
Cofactor
Zn2+ (UniProtKB | Rhea| CHEBI:29105 )
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Note: Binds 2 metal ions per subunit. The endogenous metal is unknown.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 61 | a divalent metal cation 1 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 63 | a divalent metal cation 1 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 65 | a divalent metal cation 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 66 | a divalent metal cation 2 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 150 | a divalent metal cation 2 (UniProtKB | ChEBI) | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | exonuclease activity | |
Molecular Function | metal ion binding |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameExodeoxyribonuclease WalJ
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Lactobacillales > Streptococcaceae > Streptococcus
Accessions
- Primary accessionA0A0H2ZQT7
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Peripheral membrane protein
Note: In exponentially growing cells mostly associated with the cytoplasmic side of the cell membrane.
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
More sensitive to some cephalosporin antibiotics, such as cefoperazone, cefotaxime, cefoxitin, and cefuroxime, but not the penicillin-like antibiotic amdinocillin.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000459008 | 1-269 | Exodeoxyribonuclease WalJ | |||
Sequence: MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLLAEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDSSQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAGIVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKKIYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI |
Proteomic databases
Structure
Sequence
- Sequence statusComplete
- Length269
- Mass (Da)29,917
- Last updated2015-09-16 v1
- Checksum25091FDA63DAA788
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000410 EMBL· GenBank· DDBJ | ABJ55100.1 EMBL· GenBank· DDBJ | Genomic DNA |