A0A0G4GSR5 · A0A0G4GSR5_VITBC

Function

function

Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

  • (2E)-decenoyl-[ACP] + NADPH + H+ = decanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • (2E)-dodecenoyl-[ACP] + NADPH + H+ = dodecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • (2E)-hexadecenoyl-[ACP] + NADPH + H+ = hexadecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • (2E)-hexenoyl-[ACP] + NADPH + H+ = hexanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • (2E)-octadecenoyl-[ACP] + NADPH + H+ = octadecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • (2E)-octenoyl-[ACP] + NADPH + H+ = octanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • (2E)-tetradecenoyl-[ACP] + NADPH + H+ = tetradecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • (3R)-hydroxybutanoyl-[ACP] = (2E)-butenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxydecanoyl-[ACP] = (2E)-decenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxydodecanoyl-[ACP] = (2E)-dodecenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxyhexadecanoyl-[ACP] = (2E)-hexadecenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxyhexanoyl-[ACP] = (2E)-hexenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxyoctadecanoyl-[ACP] = (2E)-octadecenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxyoctanoyl-[ACP] = (2E)-octenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxytetradecanoyl-[ACP] = (2E)-tetradecenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • 3-oxobutanoyl-[ACP] + NADPH + H+ = (3R)-hydroxybutanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxodecanoyl-[ACP] + NADPH + H+ = (3R)-hydroxydecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxododecanoyl-[ACP] + NADPH + H+ = (3R)-hydroxydodecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxohexadecanoyl-[ACP] + NADPH + H+ = (3R)-hydroxyhexadecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxohexanoyl-[ACP] + NADPH + H+ = (3R)-hydroxyhexanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxooctadecanoyl-[ACP] + NADPH + H+ = (3R)-hydroxyoctadecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxooctanoyl-[ACP] + NADPH + H+ = (3R)-hydroxyoctanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxotetradecanoyl-[ACP] + NADPH + H+ = (3R)-hydroxytetradecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • hexadecanoyl-[ACP] + malonyl-[ACP] + H+ = 3-oxooctadecanoyl-[ACP] + holo-[ACP] + CO2
    This reaction proceeds in the forward direction.
  • hexanoyl-[ACP] + malonyl-[ACP] + H+ = 3-oxooctanoyl-[ACP] + holo-[ACP] + CO2
    This reaction proceeds in the forward direction.
  • octanoyl-[ACP] + malonyl-[ACP] + H+ = 3-oxodecanoyl-[ACP] + holo-[ACP] + CO2
    This reaction proceeds in the forward direction.
  • tetradecanoyl-[ACP] + malonyl-[ACP] + H+ = 3-oxohexadecanoyl-[ACP] + holo-[ACP] + CO2
    This reaction proceeds in the forward direction.
  • hexadecanoyl-[ACP] + H2O = hexadecanoate + holo-[ACP] + H+
    This reaction proceeds in the forward direction.
    EC:3.1.2.14 (UniProtKB | ENZYME | Rhea)
  • tetradecanoyl-[ACP] + H2O = tetradecanoate + holo-[ACP] + H+
    This reaction proceeds in the forward direction.
    EC:3.1.2.14 (UniProtKB | ENZYME | Rhea)
  • a (3R)-hydroxyacyl-[ACP] + NADP+ = a 3-oxoacyl-[ACP] + NADPH + H+
    This reaction proceeds in the backward direction.
    EC:1.1.1.100 (UniProtKB | ENZYME | Rhea)
  • a (3R)-hydroxyacyl-[ACP] = a (2E)-enoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
    EC:4.2.1.59 (UniProtKB | ENZYME | Rhea)
  • a 2,3-saturated acyl-[ACP] + NADP+ = a (2E)-enoyl-[ACP] + NADPH + H+
    This reaction proceeds in the backward direction.
    EC:1.3.1.39 (UniProtKB | ENZYME | Rhea)
  • a fatty acyl-[ACP] + malonyl-[ACP] + H+ = a 3-oxoacyl-[ACP] + holo-[ACP] + CO2
    This reaction proceeds in the forward direction.
    EC:2.3.1.41 (UniProtKB | ENZYME | Rhea)
  • holo-[ACP] + acetyl-CoA = acetyl-[ACP] + CoA
    This reaction proceeds in the forward direction.
    EC:2.3.1.38 (UniProtKB | ENZYME | Rhea)
  • acetyl-[ACP] + malonyl-[ACP] + H+ = 3-oxobutanoyl-[ACP] + holo-[ACP] + CO2
    This reaction proceeds in the forward direction.
  • butanoyl-[ACP] + malonyl-[ACP] + H+ = 3-oxohexanoyl-[ACP] + holo-[ACP] + CO2
    This reaction proceeds in the forward direction.
  • decanoyl-[ACP] + malonyl-[ACP] + H+ = 3-oxododecanoyl-[ACP] + holo-[ACP] + CO2
    This reaction proceeds in the forward direction.
  • dodecanoyl-[ACP] + malonyl-[ACP] + H+ = 3-oxotetradecanoyl-[ACP] + holo-[ACP] + CO2
    This reaction proceeds in the forward direction.

Pathway

Lipid metabolism.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site1792Proton acceptor; for dehydratase activity
Active site1989Proton donor; for dehydratase activity
Active site3990Proton acceptor; for dehydratase activity
Active site4170Proton donor; for dehydratase activity

GO annotations

AspectTerm
Molecular Function(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
Molecular Function3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity
Molecular Function3-oxoacyl-[acyl-carrier-protein] synthase activity
Molecular Function[acyl-carrier-protein] S-acetyltransferase activity
Molecular Function[acyl-carrier-protein] S-malonyltransferase activity
Molecular Functionenoyl-[acyl-carrier-protein] reductase (NADPH) activity
Molecular Functionfatty acid synthase activity
Molecular Functionfatty acyl-[ACP] hydrolase activity
Molecular Functionphosphopantetheine binding
Biological Processfatty acid biosynthetic process
Biological Processphenol-containing compound biosynthetic process
Biological Processpolyketide biosynthetic process
Biological Processtoxin biosynthetic process

Keywords

Names & Taxonomy

Protein names

  • Submitted names
    • Uncharacterized protein

Gene names

    • ORF names
      Vbra_18624

Organism names

Accessions

  • Primary accession
    A0A0G4GSR5

Proteomes

Organism-specific databases

PTM/Processing

Keywords

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for region, compositional bias, domain.

TypeIDPosition(s)Description
Region1-34Disordered
Compositional bias17-34Pro residues
Region736-821Disordered
Domain832-908Carrier
Domain936-1364Ketosynthase family 3 (KS3)
Region1438-1459Disordered
Region1761-1901N-terminal hotdog fold
Domain1761-2104PKS/mFAS DH
Region1923-2104C-terminal hotdog fold
Region2126-2145Disordered
Region2232-2255Disordered
Region2845-2871Disordered
Domain3394-3469Carrier
Domain3496-3926Ketosynthase family 3 (KS3)
Region3960-4086N-terminal hotdog fold
Domain3960-4260PKS/mFAS DH
Region4104-4260C-terminal hotdog fold
Region4285-4307Disordered
Compositional bias4286-4306Basic and acidic residues
Domain5188-5263Carrier
Domain5291-5721Ketosynthase family 3 (KS3)
Region6089-6256N-terminal hotdog fold
Domain6089-6434PKS/mFAS DH
Region6179-6218Disordered
Region6275-6434C-terminal hotdog fold
Region7252-7279Disordered
Domain7318-7394Carrier

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    8,182
  • Mass (Da)
    880,699
  • Last updated
    2015-09-16 v1
  • Checksum
    CDA5823FFE48DC0B

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias17-34Pro residues
Compositional bias4286-4306Basic and acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CDMY01000791
EMBL· GenBank· DDBJ
CEM33720.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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