A0A0G4GSR5 · A0A0G4GSR5_VITBC
- ProteinUncharacterized protein
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids8182 (go to sequence)
- Protein existencePredicted
- Annotation score5/5
Function
function
Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain.
Catalytic activity
- (2E)-butenoyl-[ACP] + NADPH + H+ = butanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- (2E)-decenoyl-[ACP] + NADPH + H+ = decanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- (2E)-dodecenoyl-[ACP] + NADPH + H+ = dodecanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- (2E)-hexadecenoyl-[ACP] + NADPH + H+ = hexadecanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- (2E)-hexenoyl-[ACP] + NADPH + H+ = hexanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- (2E)-octadecenoyl-[ACP] + NADPH + H+ = octadecanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- (2E)-octenoyl-[ACP] + NADPH + H+ = octanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- (2E)-tetradecenoyl-[ACP] + NADPH + H+ = tetradecanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- (3R)-hydroxybutanoyl-[ACP] = (2E)-butenoyl-[ACP] + H2OThis reaction proceeds in the forward direction.
- (3R)-hydroxydecanoyl-[ACP] = (2E)-decenoyl-[ACP] + H2OThis reaction proceeds in the forward direction.
- (3R)-hydroxydodecanoyl-[ACP] = (2E)-dodecenoyl-[ACP] + H2OThis reaction proceeds in the forward direction.
- (3R)-hydroxyhexadecanoyl-[ACP] = (2E)-hexadecenoyl-[ACP] + H2OThis reaction proceeds in the forward direction.
- (3R)-hydroxyhexanoyl-[ACP] = (2E)-hexenoyl-[ACP] + H2OThis reaction proceeds in the forward direction.
- (3R)-hydroxyoctadecanoyl-[ACP] = (2E)-octadecenoyl-[ACP] + H2OThis reaction proceeds in the forward direction.
- (3R)-hydroxyoctanoyl-[ACP] = (2E)-octenoyl-[ACP] + H2OThis reaction proceeds in the forward direction.
- (3R)-hydroxytetradecanoyl-[ACP] = (2E)-tetradecenoyl-[ACP] + H2OThis reaction proceeds in the forward direction.
- 3-oxobutanoyl-[ACP] + NADPH + H+ = (3R)-hydroxybutanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- 3-oxodecanoyl-[ACP] + NADPH + H+ = (3R)-hydroxydecanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- 3-oxododecanoyl-[ACP] + NADPH + H+ = (3R)-hydroxydodecanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- 3-oxohexadecanoyl-[ACP] + NADPH + H+ = (3R)-hydroxyhexadecanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- 3-oxohexanoyl-[ACP] + NADPH + H+ = (3R)-hydroxyhexanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- 3-oxooctadecanoyl-[ACP] + NADPH + H+ = (3R)-hydroxyoctadecanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- 3-oxooctanoyl-[ACP] + NADPH + H+ = (3R)-hydroxyoctanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- 3-oxotetradecanoyl-[ACP] + NADPH + H+ = (3R)-hydroxytetradecanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- hexadecanoyl-[ACP] + malonyl-[ACP] + H+ = 3-oxooctadecanoyl-[ACP] + holo-[ACP] + CO2This reaction proceeds in the forward direction.
- hexanoyl-[ACP] + malonyl-[ACP] + H+ = 3-oxooctanoyl-[ACP] + holo-[ACP] + CO2This reaction proceeds in the forward direction.
- octanoyl-[ACP] + malonyl-[ACP] + H+ = 3-oxodecanoyl-[ACP] + holo-[ACP] + CO2This reaction proceeds in the forward direction.
- tetradecanoyl-[ACP] + malonyl-[ACP] + H+ = 3-oxohexadecanoyl-[ACP] + holo-[ACP] + CO2This reaction proceeds in the forward direction.
- acetyl-[ACP] + malonyl-[ACP] + H+ = 3-oxobutanoyl-[ACP] + holo-[ACP] + CO2This reaction proceeds in the forward direction.
- butanoyl-[ACP] + malonyl-[ACP] + H+ = 3-oxohexanoyl-[ACP] + holo-[ACP] + CO2This reaction proceeds in the forward direction.
- decanoyl-[ACP] + malonyl-[ACP] + H+ = 3-oxododecanoyl-[ACP] + holo-[ACP] + CO2This reaction proceeds in the forward direction.
- dodecanoyl-[ACP] + malonyl-[ACP] + H+ = 3-oxotetradecanoyl-[ACP] + holo-[ACP] + CO2This reaction proceeds in the forward direction.
Pathway
Lipid metabolism.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 1792 | Proton acceptor; for dehydratase activity | ||||
Sequence: H | ||||||
Active site | 1989 | Proton donor; for dehydratase activity | ||||
Sequence: D | ||||||
Active site | 3990 | Proton acceptor; for dehydratase activity | ||||
Sequence: H | ||||||
Active site | 4170 | Proton donor; for dehydratase activity | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity | |
Molecular Function | 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity | |
Molecular Function | 3-oxoacyl-[acyl-carrier-protein] synthase activity | |
Molecular Function | [acyl-carrier-protein] S-acetyltransferase activity | |
Molecular Function | [acyl-carrier-protein] S-malonyltransferase activity | |
Molecular Function | enoyl-[acyl-carrier-protein] reductase (NADPH) activity | |
Molecular Function | fatty acid synthase activity | |
Molecular Function | fatty acyl-[ACP] hydrolase activity | |
Molecular Function | phosphopantetheine binding | |
Biological Process | fatty acid biosynthetic process | |
Biological Process | phenol-containing compound biosynthetic process | |
Biological Process | polyketide biosynthetic process | |
Biological Process | toxin biosynthetic process |
Keywords
- Molecular function
- Ligand
Names & Taxonomy
Protein names
- Submitted names
Gene names
Organism names
- Taxonomic lineageEukaryota > Sar > Alveolata > Colpodellida > Vitrellaceae > Vitrella
Accessions
- Primary accessionA0A0G4GSR5
Proteomes
Organism-specific databases
PTM/Processing
Keywords
- PTM
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-34 | Disordered | ||||
Sequence: MEEPFPRASDDVAHQPGVPPPHAPSPSPSHQFPA | ||||||
Compositional bias | 17-34 | Pro residues | ||||
Sequence: GVPPPHAPSPSPSHQFPA | ||||||
Region | 736-821 | Disordered | ||||
Sequence: HSASGSMQQQPHPASGGDDGGVCDAHEASSPSVPTDGGEVASGNVNHPHGGPGHLHTSLSSTRLVGESAATDDGAPETPEPTEGGE | ||||||
Domain | 832-908 | Carrier | ||||
Sequence: VSQREIQEVIVSALGEIVRLPDRPGLDCPLHELGIDSIAAVNFCNLLGKRLSRDFPATLLFDYPTIRDLTNYIMEDE | ||||||
Domain | 936-1364 | Ketosynthase family 3 (KS3) | ||||
Sequence: DRGIAVVSTACRFPCGVSSSKQFWEFLMDGCDGITAVPLSRWDMHVYYDGEGDNTGDRCYVQHGGFIEDIEFFDAGVFGIATAEVWSMDPQQRCVLEVGYEALHAAGFTRESLLGGDTGVFVGCCSFDWSNMMHKCIAHASPFSSTGSAASILANRMSYVFGLKGPSMTIDTACSSSLVAVDMASRHLLRGYGKAVMAGGVNLMLAPHSFIAFCKAGMLAPDGKCKTFDEAANGYSRGDGCGMVVMKRQSDARDNEDDILAVLRGSGVNQDGRSASLTAPNGPSQQRCIRAALDDAGVGASEISFVECHGTGTPLGDPMEVRSLHAVFAKAARTAPLVLGAVKTNVGHSEGASGIAGLIKTVLVLQHGQVPPNLHFKQLNHHIASLLQGFPVHFPAKATRLAAVKGQRLKAGVQSFGFGGTNAHCVLEQ | ||||||
Region | 1438-1459 | Disordered | ||||
Sequence: PLQSSAPPLSSSPSKDQSHPLS | ||||||
Region | 1761-1901 | N-terminal hotdog fold | ||||
Sequence: HPLLSSRTHEPDGMTVYTMQLSPRLFELFSHHEVASKAVFPAAGQLEMVAAAARLDRLVPSEDADRPSLFDDCRPGSHHLCVELHDVSFEAPIVMDEEEGTMQPLRCQLKDGRFAITTIKVTDGSEFIHASGSVDVALESL | ||||||
Domain | 1761-2104 | PKS/mFAS DH | ||||
Sequence: HPLLSSRTHEPDGMTVYTMQLSPRLFELFSHHEVASKAVFPAAGQLEMVAAAARLDRLVPSEDADRPSLFDDCRPGSHHLCVELHDVSFEAPIVMDEEEGTMQPLRCQLKDGRFAITTIKVTDGSEFIHASGSVDVALESLHDDFPVSHHQVDPKTLQQETCTEPVDIPHLYEGLRTNGLVYGPRFRTLRQAWVGTGDTRGQGLGLVRHGGAGLELFERLFGFHPTILDGSLHVVACILAAEMAPPNVKEREDAEDDDGEMKPATLHYDGIFVPSSVKRVSIGSAPLGDSVWSHVRVRPQASGDIMSLDVTTLVADVTLLTDSGSVLGEIETLTLRKLHSTSKI | ||||||
Region | 1923-2104 | C-terminal hotdog fold | ||||
Sequence: TEPVDIPHLYEGLRTNGLVYGPRFRTLRQAWVGTGDTRGQGLGLVRHGGAGLELFERLFGFHPTILDGSLHVVACILAAEMAPPNVKEREDAEDDDGEMKPATLHYDGIFVPSSVKRVSIGSAPLGDSVWSHVRVRPQASGDIMSLDVTTLVADVTLLTDSGSVLGEIETLTLRKLHSTSKI | ||||||
Region | 2126-2145 | Disordered | ||||
Sequence: DASLAQQRHGPDASSPSLSP | ||||||
Region | 2232-2255 | Disordered | ||||
Sequence: GDSYASATPTFSPSTLQDNISPAA | ||||||
Region | 2845-2871 | Disordered | ||||
Sequence: DQGQSPSAGGQRDTEGGASAAQPQDGK | ||||||
Domain | 3394-3469 | Carrier | ||||
Sequence: PERKQWLQSVVLDAVEKSSSVRPSLSLPLMELGIDSLAAVELRNELSRRLGIRLPATLLFDFPTVGQIVDFVYEEA | ||||||
Domain | 3496-3926 | Ketosynthase family 3 (KS3) | ||||
Sequence: ADALVISGMDCRMPGCEESPGGFWRLLVDQVDAVRPVPLSRWDMDEIYDSNMEADGRSYCKEAAFVSHAELFDMGFFGISSAEAETMDPQQRVMMEVGYGALHTAGYRQQSVSGSNTGVFIGCCGYDWSYRLVDAKSIGTYTGTGVAVSILANRISFALGLRGPSLTIDTACSSSLVALDAACQSLRSHRCRTALVGGVQLMLSGQGLVVFCKSKMMSPSGRCKTFDASADGFGRGEGCGAVVVESMHSLTDRAAGRHALAMVRGSAVNQDGRSATLMAPNGPAQQDCIRRALQDGDVRPEDVVFIETHGTGTQLGDPMEVGALKAVLVDNHRSNHQPLFLGSVKSNIGHLFGAAGIAGLIKTILVFQHGQVPPNVHLKQLSPHLDFDSVITLPTGMAALPTYPEDSGRKSMGGVSSFGFGGTNAHVIISA | ||||||
Region | 3960-4086 | N-terminal hotdog fold | ||||
Sequence: HPFLKEKAARDGRTAYLCPVDAKVFGVVCDHVILSRIIWPAAGYLEMAAAVARDADRKRRRSGVVTLEDVSIDNPLLLRRPPDDTDRQATWTGPVLSCDIDPTDGAFEMTASDEHLVAGRALMDETE | ||||||
Domain | 3960-4260 | PKS/mFAS DH | ||||
Sequence: HPFLKEKAARDGRTAYLCPVDAKVFGVVCDHVILSRIIWPAAGYLEMAAAVARDADRKRRRSGVVTLEDVSIDNPLLLRRPPDDTDRQATWTGPVLSCDIDPTDGAFEMTASDEHLVAGRALMDETEGAGLADIPPLQEIRQRLTTEISPHHFYSTVAEAGLQLQRKFQVLKELYWDPSSLEVLGRAEVDTDLHLGTSFESSFGIHPALLDGTLQLPAMGILATPEGHTPDLFVPAGIKTATISFKTQPHSRTLWSHIKVGARDTEKGTLDIQMTVWDGEGRAVAAFQHFTYQQVKMGRTM | ||||||
Region | 4104-4260 | C-terminal hotdog fold | ||||
Sequence: TTEISPHHFYSTVAEAGLQLQRKFQVLKELYWDPSSLEVLGRAEVDTDLHLGTSFESSFGIHPALLDGTLQLPAMGILATPEGHTPDLFVPAGIKTATISFKTQPHSRTLWSHIKVGARDTEKGTLDIQMTVWDGEGRAVAAFQHFTYQQVKMGRTM | ||||||
Region | 4285-4307 | Disordered | ||||
Sequence: GTQTRNTEDEAAPADPSAEKGHA | ||||||
Compositional bias | 4286-4306 | Basic and acidic residues | ||||
Sequence: TQTRNTEDEAAPADPSAEKGH | ||||||
Domain | 5188-5263 | Carrier | ||||
Sequence: QFVQTTVSQAAKDVLSMQADEMPSPDTPLGELGLDSLSAVEFRNRINEQLGVKLPPTAMFDFPTITKLVEYIYEEV | ||||||
Domain | 5291-5721 | Ketosynthase family 3 (KS3) | ||||
Sequence: GVGFAITGMSCRFPGGSNSIAAFWQALMDKKDAVIPIPFSRWDGDVVYNPDMDSDGRSSYTRMASFLDHPERFDCTFFGVSRDEAWYLDPQQRLMLEVGYEALDQAGHDKYDLAGEMIGCFIGVCNSDWAGVMRGTKATAYTGTAVALSLCANRVSYTLGLKGPSMCFDTACSSALVACDAACLNLRGGRCSEALVGGTQVLSTTQTFVAFSQSKMLSPDGHCKTFDAGANGFGRGEGTGAVVLSRLADVEKEGRLMWGILRGAAVNQDGRSATLMAPNGPSQQQVLRMALAEGRVDPRDVAYIEAHGTGTVLGDPIELGALQTVLPRRSPGAPPLVIASGKTNINHLEGSAGIAGLIKLVLVLQNQIAPANIHLKALNPLIGVEEWDVVFPTECMRLTPTRHDDNGAATLVGGVSSFGFGGTNSHAVVEC | ||||||
Region | 6089-6256 | N-terminal hotdog fold | ||||
Sequence: HPMVEQTIAVANSFSQRSVIYATSPHGRPQPLIRDHLTQNERIYPTSFLVEAAMAAYALSQTALDEEFGVRSYLAEDACVCAMNLRIAQHPSVETPQPGPSPSLALGETSDAKDEAAAQPAATPEVWPTLPSFPIDVQRGGTVVIRRDGSSQDSDTEQGILTAEVRVD | ||||||
Domain | 6089-6434 | PKS/mFAS DH | ||||
Sequence: HPMVEQTIAVANSFSQRSVIYATSPHGRPQPLIRDHLTQNERIYPTSFLVEAAMAAYALSQTALDEEFGVRSYLAEDACVCAMNLRIAQHPSVETPQPGPSPSLALGETSDAKDEAAAQPAATPEVWPTLPSFPIDVQRGGTVVIRRDGSSQDSDTEQGILTAEVRVDERVQEDAGVHIGSLASRCPIHVASGDFYALLGSLGTGVGKAFALASGIRYGERVITATLRLHEARDDHEAFEEGFLFHPAFLESGFHLTAFLMAVTELGVDPQKPASATPVGYLPRSVAEVMITNMPLCEGTDTELLICVQLDALSGRADMHLYRREGGCVASMRGIELQPYKATVEE | ||||||
Region | 6179-6218 | Disordered | ||||
Sequence: PSVETPQPGPSPSLALGETSDAKDEAAAQPAATPEVWPTL | ||||||
Region | 6275-6434 | C-terminal hotdog fold | ||||
Sequence: PIHVASGDFYALLGSLGTGVGKAFALASGIRYGERVITATLRLHEARDDHEAFEEGFLFHPAFLESGFHLTAFLMAVTELGVDPQKPASATPVGYLPRSVAEVMITNMPLCEGTDTELLICVQLDALSGRADMHLYRREGGCVASMRGIELQPYKATVEE | ||||||
Region | 7252-7279 | Disordered | ||||
Sequence: PQAKRQAGRSRTGQRQHAQREAMGAAQD | ||||||
Domain | 7318-7394 | Carrier | ||||
Sequence: ERRRVVESVVLSIAEEVLAVSEMPPMDMPLQELGIDSMAAVELRNALSRRLGVKLPPNVLFDFPTIEDIITFVLGDL |
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length8,182
- Mass (Da)880,699
- Last updated2015-09-16 v1
- ChecksumCDA5823FFE48DC0B
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 17-34 | Pro residues | ||||
Sequence: GVPPPHAPSPSPSHQFPA | ||||||
Compositional bias | 4286-4306 | Basic and acidic residues | ||||
Sequence: TQTRNTEDEAAPADPSAEKGH |
Keywords
- Technical term