A0A0G2KQY6 · S39AE_DANRE
- ProteinMetal cation symporter ZIP14
- Geneslc39a14
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids494 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score5/5
Function
function
Broad-scope metal ion transporter with a preference for zinc uptake. Also mediates cellular uptake of nontransferrin-bound iron.
Electroneutral transporter of the plasma membrane mediating the cellular uptake of the divalent metal cations zinc, manganese and iron that are important for tissue homeostasis, metabolism, development and immunity (PubMed:27231142).
Functions as an energy-dependent symporter, transporting through the membranes an electroneutral complex composed of a divalent metal cation and two bicarbonate anions (By similarity).
Beside these endogenous cellular substrates, can also import cadmium a non-essential metal which is cytotoxic and carcinogenic (By similarity).
Functions as an energy-dependent symporter, transporting through the membranes an electroneutral complex composed of a divalent metal cation and two bicarbonate anions (By similarity).
Beside these endogenous cellular substrates, can also import cadmium a non-essential metal which is cytotoxic and carcinogenic (By similarity).
Catalytic activity
- 2 hydrogencarbonate(out) + Zn2+(out) = 2 hydrogencarbonate(in) + Zn2+(in)This reaction proceeds in the forward direction.
2 hydrogencarbonate (out)CHEBI:17544+ Zn2+ (out)CHEBI:29105= 2 hydrogencarbonate (in)CHEBI:17544+ Zn2+ (in)CHEBI:29105 - 2 hydrogencarbonate(out) + Mn2+(out) = 2 hydrogencarbonate(in) + Mn2+(in)This reaction proceeds in the forward direction.
- Fe2+(out) + 2 hydrogencarbonate(out) = Fe2+(in) + 2 hydrogencarbonate(in)This reaction proceeds in the forward direction.
- Cd2+(out) + 2 hydrogencarbonate(out) = Cd2+(in) + 2 hydrogencarbonate(in)This reaction proceeds in the forward direction.
GO annotations
Keywords
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameMetal cation symporter ZIP14
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Actinopterygii > Neopterygii > Teleostei > Ostariophysi > Cypriniformes > Danionidae > Danioninae > Danio
Accessions
- Primary accessionA0A0G2KQY6
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Apical cell membrane ; Multi-pass membrane protein
Basolateral cell membrane ; Multi-pass membrane protein
Early endosome membrane ; Multi-pass membrane protein
Late endosome membrane ; Multi-pass membrane protein
Lysosome membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 35-152 | Extracellular | ||||
Sequence: QDGASPAQVLQELLTRYGDNASISVPQLRSLLVRLNGGQSEDHDSKTQPTRTNASKCLAADTLAVYGMSEQSRIDERGLQQICPTMIQQLDSQACKTQPNQESESSPRPTEAEVWGYG | ||||||
Transmembrane | 153-173 | Helical | ||||
Sequence: LLCVTVISLCSLVGASVVPFM | ||||||
Topological domain | 174-181 | Cytoplasmic | ||||
Sequence: RKTFYKRL | ||||||
Transmembrane | 182-202 | Helical | ||||
Sequence: LLYFIALAIGTLYSNALFQLI | ||||||
Topological domain | 203-219 | Extracellular | ||||
Sequence: PEAFGFDPMEDYYVPKS | ||||||
Transmembrane | 220-240 | Helical | ||||
Sequence: AVVFGGFYLFFFTEKILKMIL | ||||||
Topological domain | 241-397 | Cytoplasmic | ||||
Sequence: KPKDTGGHGHGHSHFPAERYANSNGDLEDGVMEKLQNGEAGGAALPRAEADGRGVGEDDKMLSTGQTVQDTQSSGGGGTGGCYWLKGRAYSDIGTLAWMITLSDGLHNFIDGLAIGASFTASVFQGISTSVAILCEEFPHELGDFVILLNAGMSIQQ | ||||||
Transmembrane | 398-418 | Helical | ||||
Sequence: ALFFNFLSACCCYLGMGFGIL | ||||||
Topological domain | 419-426 | Extracellular | ||||
Sequence: AGNNFSPN | ||||||
Transmembrane | 427-447 | Helical | ||||
Sequence: WIFALAGGMFLYIALADMFPE | ||||||
Topological domain | 448-462 | Cytoplasmic | ||||
Sequence: MNEVSREEEEAGGSG | ||||||
Transmembrane | 463-483 | Helical | ||||
Sequence: FLLTFALQNAGLLTGFAIMLV | ||||||
Topological domain | 484-494 | Extracellular | ||||
Sequence: LTIYSGQIQLG |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Embryos with CRISPR-induced slc39a14 null mutations display altered manganese homeostasis. This is associated with manganese deposition in the brain and altered locomotor activity. Mutants survived into adulthood without any obvious morphological or developmental defects.
PTM/Processing
Features
Showing features for signal, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-34 | |||||
Sequence: MTLRRASGCRQLTLTIGLALTLGLLQWPIGDVRG | ||||||
Chain | PRO_5002547407 | 35-494 | Metal cation symporter ZIP14 | |||
Sequence: QDGASPAQVLQELLTRYGDNASISVPQLRSLLVRLNGGQSEDHDSKTQPTRTNASKCLAADTLAVYGMSEQSRIDERGLQQICPTMIQQLDSQACKTQPNQESESSPRPTEAEVWGYGLLCVTVISLCSLVGASVVPFMRKTFYKRLLLYFIALAIGTLYSNALFQLIPEAFGFDPMEDYYVPKSAVVFGGFYLFFFTEKILKMILKPKDTGGHGHGHSHFPAERYANSNGDLEDGVMEKLQNGEAGGAALPRAEADGRGVGEDDKMLSTGQTVQDTQSSGGGGTGGCYWLKGRAYSDIGTLAWMITLSDGLHNFIDGLAIGASFTASVFQGISTSVAILCEEFPHELGDFVILLNAGMSIQQALFFNFLSACCCYLGMGFGILAGNNFSPNWIFALAGGMFLYIALADMFPEMNEVSREEEEAGGSGFLLTFALQNAGLLTGFAIMLVLTIYSGQIQLG |
Expression
Developmental stage
Expressed at all stages of embryonic and early larval development.
Gene expression databases
Interaction
Structure
Family & Domains
Features
Showing features for motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 248-255 | HHHGHXHX-motif | ||||
Sequence: HGHGHSHF | ||||||
Motif | 376-381 | XEXPHE-motif | ||||
Sequence: EEFPHE |
Sequence similarities
Belongs to the ZIP transporter (TC 2.A.5) family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length494
- Mass (Da)53,266
- Last updated2015-07-22 v1
- Checksum313E3DD0E8213C08
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A2R8Q9W7 | A0A2R8Q9W7_DANRE | slc39a14 | 494 | ||
A0A8M9Q926 | A0A8M9Q926_DANRE | slc39a14 | 494 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CABZ01088693 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
CABZ01088694 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
CABZ01088695 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
CABZ01088696 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
CABZ01088697 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
CABZ01088698 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. |