A0A0G2K1Q8 · ABCA3_RAT

  • Protein
    Phospholipid-transporting ATPase ABCA3
  • Gene
    Abca3
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Catalyzes the ATP-dependent transport of phospholipids such as phosphatidylcholine and phosphoglycerol from the cytoplasm into the lumen side of lamellar bodies, in turn participates in the lamellar bodies biogenesis and homeostasis of pulmonary surfactant. Transports preferentially phosphatidylcholine containing short acyl chains. In addition plays a role as an efflux transporter of miltefosine across macrophage membranes and free cholesterol (FC) through intralumenal vesicles by removing FC from the cell as a component of surfactant and protects cells from free cholesterol toxicity.

Catalytic activity

Features

Showing features for site, binding site.

TypeIDPosition(s)Description
Site174-175Cleavage; by CTSL
Binding site566-573ATP (UniProtKB | ChEBI)
Binding site1416-1423ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentalveolar lamellar body
Cellular Componentalveolar lamellar body membrane
Cellular Componentcytoplasmic vesicle membrane
Cellular Componentintracellular membrane-bounded organelle
Cellular Componentlamellar body
Cellular Componentlamellar body membrane
Cellular Componentlate endosome
Cellular Componentlysosomal membrane
Cellular Componentmultivesicular body membrane
Cellular Componentplasma membrane
Molecular FunctionABC-type xenobiotic transporter activity
Molecular FunctionATP binding
Molecular FunctionATP hydrolysis activity
Molecular FunctionATPase-coupled transmembrane transporter activity
Molecular Functionlipid transporter activity
Molecular Functionphosphatidylcholine flippase activity
Molecular Functionphosphatidylcholine transfer activity
Biological Processlipid transport
Biological Processlung development
Biological Processorganelle assembly
Biological Processphosphatidylcholine metabolic process
Biological Processphosphatidylglycerol metabolic process
Biological Processphospholipid homeostasis
Biological Processphospholipid transport
Biological Processpositive regulation of cholesterol efflux
Biological Processpositive regulation of phospholipid efflux
Biological Processpositive regulation of phospholipid transport
Biological Processpositive regulation of protein homooligomerization
Biological Processregulation of lipid biosynthetic process
Biological Processregulation of lipid transport
Biological Processregulation of phosphatidylcholine metabolic process
Biological Processresponse to glucocorticoid
Biological Processsurfactant homeostasis
Biological Processxenobiotic export from cell
Biological Processxenobiotic transmembrane transport
Biological Processxenobiotic transport

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Phospholipid-transporting ATPase ABCA3
  • EC number
  • Alternative names
    • ATP-binding cassette sub-family A member 3
    • Xenobiotic-transporting ATPase ABCA3
      (EC:7.6.2.2
      ) . EC:7.6.2.2 (UniProtKB | ENZYME | Rhea)
  • Cleaved into 1 chains

Gene names

    • Name
      Abca3

Organism names

  • Taxonomic identifier
  • Strain
    • Brown Norway
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus

Accessions

  • Primary accession
    A0A0G2K1Q8

Proteomes

Organism-specific databases

Subcellular Location

Endosome, multivesicular body membrane
; Multi-pass membrane protein
Lysosome membrane
Note: Localized in the limiting membrane of lamellar bodies in lung alveolar type II cells. Trafficks via the Golgi, sorting vesicles (SVs) and late endosome/multivesicular body network directly to the outer membrane of lamellar bodies in AT2 lung epithelial cells or to lysosomes and lysosomal-related organelles (LROs) in other cells where undergoes proteolytic cleavage and oligosaccharide processing from high mannose type to complex type. Oligomers formation takes place in a post-endoplasmic reticulum compartment.

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane22-42Helical
Transmembrane251-271Helical
Transmembrane307-327Helical
Transmembrane344-364Helical
Transmembrane373-393Helical
Transmembrane405-425Helical
Transmembrane1100-1120Helical
Transmembrane1144-1164Helical
Transmembrane1183-1203Helical
Transmembrane1213-1233Helical
Transmembrane1245-1265Helical
Transmembrane1310-1330Helical

Keywords

PTM/Processing

Features

Showing features for chain, glycosylation.

TypeIDPosition(s)Description
ChainPRO_00004522991-1704Phospholipid-transporting ATPase ABCA3
Glycosylation14N-linked (GlcNAc...) asparagine
Glycosylation53N-linked (GlcNAc...) asparagine
Glycosylation124N-linked (GlcNAc...) asparagine
Glycosylation140N-linked (GlcNAc...) asparagine
ChainPRO_0000452300175-1704150 Kda mature form
Glycosylation228N-linked (GlcNAc...) asparagine
Glycosylation620N-linked (GlcNAc...) asparagine
Glycosylation945N-linked (GlcNAc...) asparagine
Glycosylation1350N-linked (GlcNAc...) asparagine

Post-translational modification

N-glycosylated. Localization at intracellular vesicles is accompanied by processing of oligosaccharide from high mannose type to complex type. N-linked glycosylation at Asn-124 and Asn-140 is required for stability and efficient anterograde trafficking and prevents from proteasomal degradation.
Proteolytically cleaved by CTSL and to a lower extent by CTSB within multivesicular bodies (MVB) and lamellar bodies (LB) leading to a mature form of 150 kDa.

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Highly expressed in lung, moderately expressed in stomach, intestine, and kidney and weakly expressed in thyroid, brain, liver, spleen, heart, testis, and thymus.

Gene expression databases

Interaction

Subunit

Homooligomer; disulfide-linked.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain530-763ABC transporter 1
Domain1381-1614ABC transporter 2

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,704
  • Mass (Da)
    191,767
  • Last updated
    2015-07-22 v1
  • Checksum
    A1F47B43E9CEB8CC
MVVLRQLRLLLWKNYTLKKRKVLVTVLELFLPLLFSGILIWLRLKIQSENVPNATVYPDQHIQELPLFFSFPPPGGSWELAYVPSHSDAARTITEAVRREFMIKMRVHGFSSEKDFEDYVRYDNHSSNVLAAVVFEHTFNHSKDPLPLAVRYHLRFSYTRRNYMWTQTGNLFLKETEGWHTASLFPLFPSPGPREPSSPDGGEPGYIREGFLAVQHAVDKAIMHYHANASAHQLFQKLTVITKRFPFPPYISDPFLIAIQYQLPLLLMLSFTYTSLTIIRAVVQEKEKKLKEYMRMMGLSSWLHWSAWFLMFLLFSLIVVSFMTLLFCVKVKKDIAVLSNSDPSLVLAFLLCFAISSISFSFMVSTFFSKANMAATVGGFLYFFTYTPYFFVAPRYNWMTLSQKLLSCLLSNVAMAMGAQLIGKFEAKGTGIQWCDLLNPVNVDDDFCFGQVLGMLLLDSVLYGLVTWYVEAVFPGQFGVPQPWYFFLMPSYWCGNPRTVVGKEEEGGDPEKAFRTEYFEAEPEDLAAGIKIKHLSKVFQVGNKDKMGIRDLTLNLYEGQITVLLGHNGAGKTTTMSMLTGLFPPTSGHAYIRGYEISQDMVQIRKSLGLCPQHDVLFDNLTVAEHLYFYAQLKGLSVQKCPEEVKQMLHTLGLEDKRDSRSKFLSGGMKRKLAIGIALIAGSKVLMLDEPTSGMDAVSRRAIWDLLQQQKSDRTVLLTTHFMDEADLLGDRIAILAKGELQCCGSSLFLKQKYGAGYHMTLVKEPHCNPEGISQLVHHHVPNAMLESHAGAELSFILPKESTHRFESLFAKLEKKQKELGIASFGASVTTMEEVFLRVGKLVDTSMDIQAIQLPALQYQHERRASDWALDSNLCGVMDPTNGIGALIEEEEVLVKLNTGLALHCQQFWAMFLKKAAYSWREWRMVAAQILVPVTCLTLALLAINYTSEIFDDPPLKLSLNEYGTTVVPFSVPGTSRLGQQLSEHLRDMLQAERQEPREVLGDLEEFLVFRASVEGGGFNERCLVATSFKDSGERTVVTALFNNQAYHSPATALAIVDNLLFKLLCGPRASIEISNYPQPRSTLQVAKDQFNEGRKGFDIALNLLIAMAFLASTFSILAVSERAVQAKHVQFVSGVHVATFWLSALLWDLISFLVPSLLLLVVFRAFDVHAFTRDGHMADLLLLLMLYGWAIIPLMYLLSFFFSAASTAYTRLTIFNILSGIATFIVVTIMRIPAVKLEELSRTLDHVFLVLPNHCLGMAVSNFYENYETRRYCTSSEVATHYCKKYNIQYQENFYAWSTPGIGKFVTSMAASGGIYLTLLFLIETNLLWRLRTFVCAFRRRWTLAELQNRTSVLPEDQDVADERSRVLVPSLDSMLDTPLIINELSKVYDQRAPLLAVDRISLAVQKGECFGLLGFNGAGKTTTFKMLTGEETITSGDAFVGGYSISSDIGKVRQRMGYCPQFDALLDHMTGREMLVMYARLRGIPERLIDACVENTLRGLLLEPHANKLVKTYSGGNKRKLSTGIALIGEPAVIFLDEPSTGMDPVARRLLWDTVARARESGKAIVITSHSMEECEALCTRLAIMVQGQFKCLGSPQHLKSKFGSGYSLQAKVRSEGKQEVLEEFKAFVDLTFPGSVLEDEHQDMVHYHLPGCDLSWAKVFGILEKAKEKYGVDDYSVSQISLEQVFLSFAHLQPPTTEDGR

Computationally mapped potential isoform sequences

There are 3 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A8I6AIR0A0A8I6AIR0_RATAbca31686
A0A8I6AQZ2A0A8I6AQZ2_RATAbca31449
Q5M866Q5M866_RATAbca3152

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AC098526
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
AC103090
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.

Genome annotation databases

Similar Proteins

Disclaimer

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