Essential maintenance is planned to begin on Fri Jan 24 2025. The website may be temporarily unavailable. Please use our fallback: https://wwwdev.ebi.ac.uk/uniprot/front-end/fallback/ in case of any outage.

A0A0G1AJ23 · A0A0G1AJ23_9BACT

Function

function

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Fe2+ (UniProtKB | Rhea| CHEBI:29033 )

Note: Binds 1 Fe2+ ion per subunit.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site180Fe cation (UniProtKB | ChEBI)
Binding site184Fe cation (UniProtKB | ChEBI)
Binding site216-220substrate
Binding site253substrate
Binding site266substrate
Binding site394substrate
Binding site431Fe cation (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functioniron ion binding
Molecular FunctionN(6)-L-threonylcarbamoyladenine synthase activity
Biological ProcesstRNA threonylcarbamoyladenosine modification

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA N6-adenosine threonylcarbamoyltransferase
  • EC number
  • Alternative names
    • N6-L-threonylcarbamoyladenine synthase
      (t(6)A synthase
      )
    • t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaD
    • tRNA threonylcarbamoyladenosine biosynthesis protein TsaD

Gene names

    • Name
      tsaD
    • ORF names
      UU96_C0009G0028

Organism names

Accessions

  • Primary accession
    A0A0G1AJ23

Proteomes

Subcellular Location

Keywords

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain30-75Gcp-like
Domain126-437Gcp-like

Sequence similarities

Belongs to the KAE1 / TsaD family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    463
  • Mass (Da)
    51,306
  • Last updated
    2015-07-22 v1
  • MD5 Checksum
    E1D7900EAE5C181134E72BD2989DD81C
MIILAIETSCDETAAAILKCSGKLKNPRFEILSNVVASQVKIHSQWGGVVPSLAKREHQKNLIPILIRTLKKASLLNSKSEARNSRPAFGGARFAREARRARLVPMDIGTAKFKILNSILKREPELLEKLLKFLREHQEPKIDVIAVTQGPGLEPALWVGINFAKALAIVWDKPLIAINHMEGHIASVLLKQNRGSTKHEARKIISNFEFPAVSLLASGGHTQLILINPPAGGWGEYKIIGETLDDAAGEAFDKVARMLGLGYPGGPAIATEAAKSKIRNPKHETNSKFNPPTGGQNSKFALPRPMLNSKDFNFSFSGLKTAVLYKIRDLPKEVRPQGGAKRRDEVEPQINEIRSLIAREFQQAVIDVLIKKTLRAAKEFKVKTIILGGGVAANKELRRQLKKAVSRENVKINDRKYLLKLLLPHIKFTGDNAAMIALAAYFHVKKKEFANWQTLRAQGNLRL

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
LCCQ01000009
EMBL· GenBank· DDBJ
KKS34066.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
Help