A0A0G0W4A4 · A0A0G0W4A4_9BACT

Function

function

Catalyzes the deamination of dCTP to dUTP.
Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Pathway

Pyrimidine metabolism; dTTP biosynthesis.
Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 1/2.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site105-110dCTP (UniProtKB | ChEBI)
Binding site123dCTP (UniProtKB | ChEBI)
Active site133Proton donor/acceptor
Binding site166dCTP (UniProtKB | ChEBI)
Binding site173dCTP (UniProtKB | ChEBI)
Binding site177dCTP (UniProtKB | ChEBI)
Active site395Nucleophile
Binding site415-418dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site418(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)
Binding site426dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site456-458dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site512(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular FunctiondCTP deaminase activity
Molecular Functionnucleotide binding
Molecular Functionthymidylate synthase activity
Biological ProcessdTMP biosynthetic process
Biological ProcessdTTP biosynthetic process
Biological ProcessdUMP biosynthetic process
Biological ProcessdUTP biosynthetic process
Biological Processmethylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Multifunctional fusion protein

Including 2 domains:

  • Recommended name
    Thymidylate synthase
  • EC number
  • Short names
    TS
    ; TSase
  • Recommended name
    dCTP deaminase
  • EC number
  • Alternative names
    • Deoxycytidine triphosphate deaminase

Gene names

    • Name
      thyA
    • Synonyms
      dcd
    • ORF names
      UU61_C0028G0011

Organism names

Accessions

  • Primary accession
    A0A0G0W4A4

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homodimer.
Homotrimer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain212-511Thymidylate synthase/dCMP hydroxymethylase

Sequence similarities

Belongs to the dCTP deaminase family.
Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    532
  • Mass (Da)
    60,435
  • Last updated
    2015-07-22 v1
  • Checksum
    CFCB5DF4D17002BB
MILSDTDIKKSISQGTIKINPSPDFEEQLGPCSLDLHLGNSFKIFKVSRYPFIDLKNGVNVDEMMEEVVIKDGGSFIIQPHDFVIAVTKEEITLPSNIMGRLDGRSSLARLGLVIHVTAARFDPGWRGRAVMELGNLGTVPIVLYSGMRICAMTFESISSSSEVPYLEQADHKYADQSGATASKILEEPGLKAVPMVGAKIPSANKNHPEYQYLNLLQELIDKGHEQVDAGTGVKTYSLFGKQFRFDLSQGFPLLTTKKVFWKGVVQELYWFMSGQKNIKYLVDNNVHIWDDYPYRIYREKITKGLEKEMTKEEFIEKIATDKDFAELHGNLPHVYGDMWRHWPTKTDRTIDQLQWLISNIRNDKSTHSAIVNSWNPEYLYEMAAPGEACRFPICHNMFQINANGGRLSLQLYQRSCDVFLGVPFNIASYALLTIILAKITGNEPGEFIHTFGDIHYYENHLDAVKEQLLREPLPFPTVKIDQNLREIDDFKPESVELIGYESHPPIKASLSPVGGIVTKDWKEFVQHNKKK

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
LCBH01000028
EMBL· GenBank· DDBJ
KKS06857.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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