A0A0G0GVZ8 · A0A0G0GVZ8_9BACT
- ProteinProbable dual-specificity RNA methyltransferase RlmN
- GenerlmN
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids345 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.
Miscellaneous
Reaction proceeds by a ping-pong mechanism involving intermediate methylation of a conserved cysteine residue.
Catalytic activity
Cofactor
Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 82 | Proton acceptor | |||
Binding site | 103 | [4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | |||
Binding site | 107 | [4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | |||
Binding site | 110 | [4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | |||
Binding site | 163-164 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 195 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 218-220 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 294 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Active site | 337 | S-methylcysteine intermediate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | metal ion binding | |
Molecular Function | rRNA (adenine(2503)-C2-)-methyltransferase activity | |
Molecular Function | rRNA binding | |
Molecular Function | tRNA (adenine(37)-C2)-methyltransferase activity | |
Molecular Function | tRNA binding | |
Biological Process | rRNA base methylation | |
Biological Process | tRNA methylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProbable dual-specificity RNA methyltransferase RlmN
- EC number
- Alternative names
Gene names
Organism names
- Taxonomic lineageBacteria > Candidatus Dojkabacteria
Accessions
- Primary accessionA0A0G0GVZ8
Proteomes
Subcellular Location
PTM/Processing
Keywords
- PTM
Structure
Sequence
- Sequence statusComplete
- Length345
- Mass (Da)38,641
- Last updated2015-07-22 v1
- Checksum3B174F158C58C602
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
LBTH01000067 EMBL· GenBank· DDBJ | KKQ34222.1 EMBL· GenBank· DDBJ | Genomic DNA |