A0A0G0GVZ8 · A0A0G0GVZ8_9BACT

Function

function

Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.

Miscellaneous

Reaction proceeds by a ping-pong mechanism involving intermediate methylation of a conserved cysteine residue.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • adenosine2503 in 23S rRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = 2-methyladenosine2503 in 23S rRNA + 5'-deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-adenosyl-L-homocysteine
    EC:2.1.1.192 (UniProtKB | ENZYME | Rhea)
  • adenosine37 in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = 2-methyladenosine37 in tRNA + 5'-deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-adenosyl-L-homocysteine
    EC:2.1.1.192 (UniProtKB | ENZYME | Rhea)

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Features

Showing features for active site, binding site.

Type
IDPosition(s)Description
Active site82Proton acceptor
Binding site103[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site107[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site110[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site163-164S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site195S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site218-220S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site294S-adenosyl-L-methionine (UniProtKB | ChEBI)
Active site337S-methylcysteine intermediate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionmetal ion binding
Molecular FunctionrRNA (adenine(2503)-C2-)-methyltransferase activity
Molecular FunctionrRNA binding
Molecular FunctiontRNA (adenine(37)-C2)-methyltransferase activity
Molecular FunctiontRNA binding
Biological ProcessrRNA base methylation
Biological ProcesstRNA methylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Probable dual-specificity RNA methyltransferase RlmN
  • EC number
  • Alternative names
    • 23S rRNA (adenine(2503)-C(2))-methyltransferase
    • 23S rRNA m2A2503 methyltransferase
    • Ribosomal RNA large subunit methyltransferase N
    • tRNA (adenine(37)-C(2))-methyltransferase
    • tRNA m2A37 methyltransferase

Gene names

    • Name
      rlmN
    • ORF names
      US52_C0067G0006

Organism names

Accessions

  • Primary accession
    A0A0G0GVZ8

Proteomes

Subcellular Location

Keywords

PTM/Processing

Keywords

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain89-326Radical SAM core

Sequence similarities

Belongs to the radical SAM superfamily. RlmN family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    345
  • Mass (Da)
    38,641
  • Last updated
    2015-07-22 v1
  • Checksum
    3B174F158C58C602
MIENFAEKHKLPGFRIKQFDKAFYQEAINSFDELTTWPKDLREKLNDEGPFSSLDPINQLKSNDGKTTKILFRRKVDKKCIESVLMRFKDGRNSVCVSCMVGCPVGCKFCATGKMGFGGNLSADEIVDQVLYFQRLLKSKGNRVNGNKTLDAHLSNIVFMGMGEPMLNLKEVQKALAILTDPSKVALSERRITISTSGYVPQLKRLIASGFRGQIAVSLHAPNQKLREKLMPVAKQHSLDELLAVLDDYVEKTNKKVSYEYILISGVNDSDVNAQELIKLFKNRLAHINLIPYNPIPGVDFKRTDKSRVVEFAEILKQGGINTTIRITMGDDIKAACGQLASNNH

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
LBTH01000067
EMBL· GenBank· DDBJ
KKQ34222.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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