A0A0G0D1S0 · A0A0G0D1S0_9BACT
- ProteinDNA-directed RNA polymerase subunit alpha
- GenerpoA
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids329 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Catalytic activity
- RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | DNA-directed RNA polymerase complex | |
Molecular Function | DNA binding | |
Molecular Function | DNA-directed 5'-3' RNA polymerase activity | |
Molecular Function | protein dimerization activity | |
Biological Process | DNA-templated transcription |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA-directed RNA polymerase subunit alpha
- EC number
- Short namesRNAP subunit alpha
- Alternative names
Gene names
Organism names
- Taxonomic lineageBacteria
Accessions
- Primary accessionA0A0G0D1S0
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Keywords
- Cellular component
Interaction
Subunit
Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-229 | Alpha N-terminal domain (alpha-NTD) | ||||
Sequence: MSEYKIVMPSKPRVVLEEGNKGVFEIDGLYPGYGHTLGNSLRRIILSSLPGASITSIKIDGVSHEFQTLEGIKEDVIVMILNLKKTRFKMISDEPQTVTLSVKGPKEVTAGDIKTGGQVEILNPELHIVEVTGKINLNIEMKIEKGLGFISKEIYQKDKVDVGTIAVDAIFTPVRRVSYEVENMRVGDKTNHNRLRISIETDGTLTAREALSRSIEIMINQLKAIIDFK | ||||||
Domain | 21-228 | DNA-directed RNA polymerase RpoA/D/Rpb3-type | ||||
Sequence: KGVFEIDGLYPGYGHTLGNSLRRIILSSLPGASITSIKIDGVSHEFQTLEGIKEDVIVMILNLKKTRFKMISDEPQTVTLSVKGPKEVTAGDIKTGGQVEILNPELHIVEVTGKINLNIEMKIEKGLGFISKEIYQKDKVDVGTIAVDAIFTPVRRVSYEVENMRVGDKTNHNRLRISIETDGTLTAREALSRSIEIMINQLKAIIDF | ||||||
Region | 239-263 | Disordered | ||||
Sequence: KDTEEKKPARSASSTADAGGGSDFA | ||||||
Region | 262-329 | Alpha C-terminal domain (alpha-CTD) | ||||
Sequence: FADVLKTRTDSLDLSTRTLNALTGANIRTLGGLARKKREDLLEVEGIGEKGISEIKKVLGKFGLNLKD |
Domain
The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements.
Sequence similarities
Belongs to the RNA polymerase alpha chain family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length329
- Mass (Da)36,103
- Last updated2015-07-22 v1
- Checksum28CED0EBDF1CC649
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
LBQY01000010 EMBL· GenBank· DDBJ | KKP87298.1 EMBL· GenBank· DDBJ | Genomic DNA |