A0A0G0CZL7 · A0A0G0CZL7_9BACT

Function

function

Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 1 zinc ion per subunit.

Pathway

tRNA modification; tRNA-queuosine biosynthesis.

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site91Proton acceptor
Binding site91-95substrate
Binding site175substrate
Binding site263substrate
Binding site290substrate
Active site337Nucleophile
Binding site375Zn2+ (UniProtKB | ChEBI)
Binding site377Zn2+ (UniProtKB | ChEBI)
Binding site380Zn2+ (UniProtKB | ChEBI)
Binding site406Zn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functionmetal ion binding
Molecular FunctiontRNA-guanosine(34) queuine transglycosylase activity
Biological Processqueuosine biosynthetic process
Biological ProcesstRNA wobble guanine modification
Biological ProcesstRNA-guanine transglycosylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Queuine tRNA-ribosyltransferase
  • EC number
  • Alternative names
    • Guanine insertion enzyme
    • tRNA-guanine transglycosylase

Gene names

    • Name
      tgt
    • ORF names
      UR90_C0004G0001

Organism names

Accessions

  • Primary accession
    A0A0G0CZL7

Proteomes

Subcellular Location

Interaction

Subunit

Homodimer. Within each dimer, one monomer is responsible for RNA recognition and catalysis, while the other monomer binds to the replacement base PreQ1.

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain14-211tRNA-guanine15 transglycosylase-like
Domain257-439tRNA-guanine15 transglycosylase-like
Region318-324RNA binding
Region342-346RNA binding; important for wobble base 34 recognition

Sequence similarities

Belongs to the queuine tRNA-ribosyltransferase family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    441
  • Mass (Da)
    48,883
  • Last updated
    2015-07-22 v1
  • Checksum
    8E7E4E5269F6354F
MQFKIQKKLKNSLGRVGILETPHGEILTPAFVAVGTKATVKSLNPEQVRDAGTQVVLGNTYHLYLQPGDEIVRDAGGIGKFMNWKGPTMTDSGGFQVFSLGVAYGKDISKITKITDPSLLIPERFDDSDAPRLAKIGQDGVSFKSHLDGSIHYITPEKSIQIQHNLGADIIFAFDECTSPAEDLKYQEEALERTHRWAERSLEKHKNLAKGAESSKKAEEYFLEGVSQADEPRGSAPLVSGDSDPIKNDSLAFFSPALFGIVQGGRDEGLRKKSAKFIGSLDFDGFGIGGSFAKEDMSTAVKWVNEILPEEKPRHLLGIGEPEDLFMGIENGVDLFDCVAPTRLGRNGTLYTKKGKIIIMNKKFRDDYSPIEKDCICYSCKNYTKAYIAHLFHGKEMLAGTLASIHNLHFIVNLVKNARQSILDDNFYEFKENFLKSYAKN

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
LBQY01000004
EMBL· GenBank· DDBJ
KKP87454.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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