A0A0F7UYF4 · A0A0F7UYF4_TOXGV
- ProteinGlutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids1134 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).
Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).
Catalytic activity
- L-aspartyl-tRNA(Asn) + L-glutamine + ATP + H2O = L-asparaginyl-tRNA(Asn) + L-glutamate + ADP + phosphate + 2 H+
- L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H+
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | glutamyl-tRNA(Gln) amidotransferase complex | |
Cellular Component | mitochondrion | |
Molecular Function | ATP binding | |
Molecular Function | glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity | |
Molecular Function | transferase activity | |
Biological Process | glutaminyl-tRNAGln biosynthesis via transamidation | |
Biological Process | mitochondrial translation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGlutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial
- EC number
- Short namesGlu-AdT subunit B
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Sar > Alveolata > Apicomplexa > Conoidasida > Coccidia > Eucoccidiorida > Eimeriorina > Sarcocystidae > Toxoplasma
Accessions
- Primary accessionA0A0F7UYF4
Subcellular Location
PTM/Processing
Features
Showing features for signal, chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Signal | 1-26 | ||||
Chain | PRO_5002523484 | 27-1134 | Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial | ||
Interaction
Subunit
Heterotrimer of A, B and C subunits.
Subunit of the heterotrimeric GatCAB amidotransferase (AdT) complex, composed of A, B and C subunits.
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 101-121 | Disordered | |||
Region | 233-290 | Disordered | |||
Compositional bias | 249-271 | Basic and acidic residues | |||
Region | 351-386 | Disordered | |||
Region | 585-625 | Disordered | |||
Compositional bias | 591-625 | Basic and acidic residues | |||
Compositional bias | 731-752 | Basic and acidic residues | |||
Region | 731-764 | Disordered | |||
Domain | 859-1127 | Asn/Gln amidotransferase | |||
Compositional bias | 891-932 | Basic and acidic residues | |||
Region | 891-971 | Disordered | |||
Compositional bias | 947-971 | Basic and acidic residues | |||
Region | 1026-1057 | Disordered | |||
Sequence similarities
Belongs to the GatB/GatE family. GatB subfamily.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,134
- Mass (Da)121,119
- Last updated2015-07-22 v1
- MD5 Checksum24614B93D5E9E1EEF60BC768EF5C8624
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 249-271 | Basic and acidic residues | |||
Compositional bias | 591-625 | Basic and acidic residues | |||
Compositional bias | 731-752 | Basic and acidic residues | |||
Compositional bias | 891-932 | Basic and acidic residues | |||
Compositional bias | 947-971 | Basic and acidic residues | |||
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
LN714498 EMBL· GenBank· DDBJ | CEL75031.1 EMBL· GenBank· DDBJ | Genomic DNA |