A0A0F2LQQ3 · A0A0F2LQQ3_9CREN
- ProteinS-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
- Genetaw1
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids358 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Component of the wyosine derivatives biosynthesis pathway that catalyzes the condensation of N-methylguanine with 2 carbon atoms from pyruvate to form the tricyclic 4-demethylwyosine (imG-14) on guanosine-37 of tRNA(Phe).
Catalytic activity
- N1-methylguanosine37 in tRNA(Phe) + pyruvate + S-adenosyl-L-methionine = 4-demethylwyosine37 in tRNA(Phe) + 5'-deoxyadenosine + L-methionine + CO2 + H2O
RHEA-COMP:10165 CHEBI:73542 Position: 37+ CHEBI:15361 + CHEBI:59789 = RHEA-COMP:10164 CHEBI:64315 Position: 37+ CHEBI:17319 + CHEBI:57844 + CHEBI:16526 + CHEBI:15377
Cofactor
Note: Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 35 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 48 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 61 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 71 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | ||||
Sequence: C | ||||||
Binding site | 75 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | ||||
Sequence: C | ||||||
Binding site | 78 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | metal ion binding | |
Molecular Function | tRNA-4-demethylwyosine synthase activity | |
Biological Process | tRNA processing |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameS-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageArchaea > Thermoproteota > Thermoprotei > Sulfolobales > Sulfolobaceae > Candidatus Aramenus
Accessions
- Primary accessionA0A0F2LQQ3
Proteomes
Subcellular Location
Interaction
Subunit
Monomer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 54-304 | Radical SAM core | ||||
Sequence: YGIESHRCVQMTPVAAWCWFRCIHCWRLEPEDIGLEWDETKMPVSDDPEYIAERSIEEHVKAVSGYYGREGVDKELVKAASRPAHVAISLTGEPTLYERLGELIKEYHKRGMTTFLVTSGVRPDILASLEEEPTQLFVSLQAPNERKHKLINRPIVANSWNLVMETLRMMPSFSSPTVIRMTMIKGFNMSEDDAREFAKLIEISMPTYIEVKAYMHVGPSTYRLSRDAMPRHREIREFAELLAKYTGYKIL |
Sequence similarities
Belongs to the TYW1 family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length358
- Mass (Da)41,672
- Last updated2015-06-24 v1
- Checksum520FBC5BBB5AF075
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
JZWS01000019 EMBL· GenBank· DDBJ | KJR79154.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JZWS02000004 EMBL· GenBank· DDBJ | MCL7344085.1 EMBL· GenBank· DDBJ | Genomic DNA |