A0A0E3SCC6 · A0A0E3SCC6_9EURY
- ProteinDNA double-strand break repair protein Mre11
- Genemre11
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids677 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Part of the Rad50/Mre11 complex, which is involved in the early steps of DNA double-strand break (DSB) repair. The complex may facilitate opening of the processed DNA ends to aid in the recruitment of HerA and NurA. Mre11 binds to DSB ends and has both double-stranded 3'-5' exonuclease activity and single-stranded endonuclease activity.
Cofactor
Note: Binds 2 manganese ions per subunit.
Activity regulation
Nuclease activity is regulated by Rad50.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 12 | Mn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 14 | Mn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 53 | Mn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 53 | Mn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 88 | Mn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Active site | 89 | Proton donor | ||||
Sequence: H | ||||||
Binding site | 158 | Mn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 191 | Mn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 3'-5' exonuclease activity | |
Molecular Function | DNA end binding | |
Molecular Function | endonuclease activity | |
Molecular Function | manganese ion binding | |
Molecular Function | Y-form DNA binding | |
Biological Process | double-strand break repair |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA double-strand break repair protein Mre11
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageArchaea > Euryarchaeota > Stenosarchaea group > Methanomicrobia > Methanosarcinales > Methanosarcinaceae > Methanosarcina
Accessions
- Primary accessionA0A0E3SCC6
Proteomes
Interaction
Subunit
Homodimer. Forms a heterotetramer composed of two Mre11 subunits and two Rad50 subunits.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, compositional bias, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 5-193 | Calcineurin-like phosphoesterase | ||||
Sequence: IRILHTADTHLGYRQYHSEVRRQDFFKAFEMVIKDAVEMQVDAVVHAGDLFDSRNPTLEDLLETMNILSRLKTANIPFFGIVGNHESKQSTQWLDLFEEMGLAARLGKKPQMVGDVAIYGIDSVPKSKIPLYDYSGFEIPESLPENCRKLLVMHQIVEPLPFADWDCAEVIENLPFKVDAILLGDYHEY | ||||||
Compositional bias | 400-417 | Polar residues | ||||
Sequence: STSPAGLISSTTIGESEG | ||||||
Region | 400-423 | Disordered | ||||
Sequence: STSPAGLISSTTIGESEGNEPAGT | ||||||
Region | 466-504 | Disordered | ||||
Sequence: NKGLEASDKVPESSDIISESSNSISESPDESSESPHVSI | ||||||
Compositional bias | 478-504 | Polar residues | ||||
Sequence: SSDIISESSNSISESPDESSESPHVSI | ||||||
Compositional bias | 554-578 | Polar residues | ||||
Sequence: QASSLAEVPSVPEISQEISGTPVEI | ||||||
Region | 554-677 | Disordered | ||||
Sequence: QASSLAEVPSVPEISQEISGTPVEIQSEDSEERAVLGKDPGDKTKTEAGKLSGLGVEVSSVNEEGDKTEKLPDEKPADIKADIKLPDEKPVKAPDKAAKPVKQSQKKGKEKSAVPRQYNLGDYL | ||||||
Compositional bias | 579-599 | Basic and acidic residues | ||||
Sequence: QSEDSEERAVLGKDPGDKTKT | ||||||
Compositional bias | 615-666 | Basic and acidic residues | ||||
Sequence: NEEGDKTEKLPDEKPADIKADIKLPDEKPVKAPDKAAKPVKQSQKKGKEKSA |
Sequence similarities
Belongs to the MRE11/RAD32 family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length677
- Mass (Da)74,232
- Last updated2015-06-24 v1
- Checksum7AE34835D195E22E
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 400-417 | Polar residues | ||||
Sequence: STSPAGLISSTTIGESEG | ||||||
Compositional bias | 478-504 | Polar residues | ||||
Sequence: SSDIISESSNSISESPDESSESPHVSI | ||||||
Compositional bias | 554-578 | Polar residues | ||||
Sequence: QASSLAEVPSVPEISQEISGTPVEI | ||||||
Compositional bias | 579-599 | Basic and acidic residues | ||||
Sequence: QSEDSEERAVLGKDPGDKTKT | ||||||
Compositional bias | 615-666 | Basic and acidic residues | ||||
Sequence: NEEGDKTEKLPDEKPADIKADIKLPDEKPVKAPDKAAKPVKQSQKKGKEKSA |
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP009516 EMBL· GenBank· DDBJ | AKB79619.1 EMBL· GenBank· DDBJ | Genomic DNA |