A0A0E3P808 · A0A0E3P808_9EURY

Function

function

Small subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The small subunit (glutamine amidotransferase) binds and cleaves glutamine to supply the large subunit with the substrate ammonia.

Catalytic activity

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site46L-glutamine (UniProtKB | ChEBI)
Binding site231L-glutamine (UniProtKB | ChEBI)
Binding site233L-glutamine (UniProtKB | ChEBI)
Active site258Nucleophile
Binding site259L-glutamine (UniProtKB | ChEBI)
Binding site262L-glutamine (UniProtKB | ChEBI)
Binding site300L-glutamine (UniProtKB | ChEBI)
Binding site302L-glutamine (UniProtKB | ChEBI)
Binding site303L-glutamine (UniProtKB | ChEBI)
Active site343
Active site345

GO annotations

AspectTerm
Cellular Componentcarbamoyl-phosphate synthase complex
Molecular FunctionATP binding
Molecular Functioncarbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
Molecular Functionglutaminase activity
Biological Process'de novo' pyrimidine nucleobase biosynthetic process
Biological Process'de novo' UMP biosynthetic process
Biological Processarginine biosynthetic process
Biological Processglutamine metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Carbamoyl phosphate synthase small chain
  • EC number
  • Alternative names
    • Carbamoyl phosphate synthetase glutamine chain

Gene names

    • Name
      carA
    • ORF names
      MSSIT_2321

Organism names

  • Taxonomic identifier
  • Strain
    • T4/M
  • Taxonomic lineage
    Archaea > Euryarchaeota > Stenosarchaea group > Methanomicrobia > Methanosarcinales > Methanosarcinaceae > Methanosarcina

Accessions

  • Primary accession
    A0A0E3P808

Proteomes

Subcellular Location

Interaction

Subunit

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.

Family & Domains

Features

Showing features for region, domain.

Type
IDPosition(s)Description
Region1-182CPSase
Domain2-132Carbamoyl-phosphate synthase small subunit N-terminal

Sequence similarities

Belongs to the CarA family.
Belongs to the CarB family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    369
  • Mass (Da)
    39,950
  • Last updated
    2015-06-24 v1
  • Checksum
    CA672F2BA93A3C29
MMKAVLGLEDGTIIRGTGFGAEGTACGELVFTTQFTGYEEALTDPSYKGQILMFTYPLIGNYGVSGERFQSDNIHAEGLVVREACKNPYHYKSTRSIHQFLEDEGKPGIEGVDTRMLTIGARERGTMRAALITGSDDGEEAVDVARNFPQITDEELIARVTCKEPRFIPGAEGAWKGSGKPKHAVLVDLGIKRNIINNLHKRGIDLTLVPATTKPKEIAGYEPDLLFISNGPGDPEKATDAINAVKAFAGTIPVAGICFGHQIISLAMGARTYKLKFGHRGGNQPVKDLIENKIFISSQNHGYAVDADSLEGTGLYVKYLNANDKTVEGVSHKDLDIFSVQFHPEAQAGPMDTEETFFGKVVKVLGGDL

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP009506
EMBL· GenBank· DDBJ
AKB29040.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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