A0A0E3LIX5 · A0A0E3LIX5_9EURY

Function

function

Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses ammonia as a nitrogen source.

Catalytic activity

Pathway

Cofactor biosynthesis; NAD+ biosynthesis; NAD+ from deamido-NAD+ (ammonia route): step 1/1.

Features

Showing features for binding site.

126520406080100120140160180200220240260
TypeIDPosition(s)Description
Binding site39-46ATP (UniProtKB | ChEBI)
Binding site45Mg2+ (UniProtKB | ChEBI)
Binding site125deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits; in other chain
Binding site145ATP (UniProtKB | ChEBI)
Binding site150Mg2+ (UniProtKB | ChEBI)
Binding site158deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits; in other chain
Binding site165deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits
Binding site174ATP (UniProtKB | ChEBI)
Binding site196ATP (UniProtKB | ChEBI)
Binding site255-256deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits; in other chain

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular Functionglutaminase activity
Molecular Functionmetal ion binding
Molecular FunctionNAD+ synthase (glutamine-hydrolyzing) activity
Molecular FunctionNAD+ synthase activity
Biological ProcessNAD biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    NH(3)-dependent NAD(+) synthetase
  • EC number

Gene names

    • Name
      nadE
    • ORF names
      MSKOL_1004

Organism names

  • Taxonomic identifier
  • Strain
    • Kolksee
  • Taxonomic lineage
    Archaea > Euryarchaeota > Stenosarchaea group > Methanomicrobia > Methanosarcinales > Methanosarcinaceae > Methanosarcina

Accessions

  • Primary accession
    A0A0E3LIX5

Proteomes

Subcellular Location

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain18-260NAD/GMP synthase

Sequence similarities

Belongs to the NAD synthetase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    265
  • Mass (Da)
    28,870
  • Last updated
    2015-06-24 v1
  • Checksum
    46CAD3F32EC8E0E4
MPDEYQGIDSMNLEIAQNRIIDFIRNETRKAGVDGAVVGISGGIDSALAATLTVKALGKDKVLGIHMPESGLTTSEDSKDAKTLADWLGIEFQTIGISGIISAFMAAVPESESADRLSKGNLKARIRMSLLYFHANRMNRMVIGTGNKTEILLGYFTKYGDGGVDLEPIGGLYKTEVWELSRRLGVPDSLITKKPSAGLWAGQTDEAELGISYLKVDEVLKRLEQNEDPETILKTPGISAEQLNSVMNRIERSEHKRNAPPVPPN

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP009524
EMBL· GenBank· DDBJ
AKB46781.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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