A0A0E3KKA4 · A0A0E3KKA4_9ALPC

Function

function

Forms a primer, NSP9-pU, which is utilized by the polymerase for the initiation of RNA chains. Interacts with ribosome signal recognition particle RNA (SRP). Together with NSP8, suppress protein integration into the cell membrane, thereby disrupting host immune defenses.
RNA-directed RNA polymerase that catalyzes the transcription of viral genomic and subgenomic RNAs. Acts in complex with nsp7 and nsp8 to transcribe both the minus and positive strands of genomic RNA. The kinase-like NiRAN domain of NSP12 attaches one or more nucleotides to the amino terminus of NSP9, forming a covalent RNA-protein intermediate that serves as transcription/replication primer. Subgenomic RNAs (sgRNAs) are formed by discontinuous transcription: The polymerase has the ability to pause at transcription-regulating sequences (TRS) and jump to the leader TRS, resulting in a major deletion. This creates a series of subgenomic RNAs that are replicated, transcribed and translated. In addition, Nsp12 is a subunit of the viral RNA capping enzyme that catalyzes the RNA guanylyltransferase reaction for genomic and sub-genomic RNAs. Subsequently, the NiRAN domain transfers RNA to GDP, and forms the core cap structure GpppA-RNA.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for active site, binding site.

Type
IDPosition(s)Description
Active site4806
Active site4808
Active site4907
Active site4983
Active site4988
Binding site5046-5052S-adenosyl-L-methionine (UniProtKB | ChEBI)
Active site5459
Active site5474
Active site5515

GO annotations

AspectTerm
Cellular Componenthost cell endoplasmic reticulum-Golgi intermediate compartment
Cellular Componenthost cell membrane
Cellular Componenthost cell perinuclear region of cytoplasm
Cellular Componentmembrane
Molecular Function3'-5'-RNA exonuclease activity
Molecular Function5'-3' DNA helicase activity
Molecular FunctionATP binding
Molecular Functioncysteine-type deubiquitinase activity
Molecular Functioncysteine-type endopeptidase activity
Molecular Functionendonuclease activity
Molecular Functionlyase activity
Molecular FunctionmRNA (nucleoside-2'-O-)-methyltransferase activity
Molecular FunctionmRNA 5'-cap (guanine-N7-)-methyltransferase activity
Molecular Functionomega peptidase activity
Molecular FunctionRNA binding
Molecular FunctionRNA helicase activity
Molecular FunctionRNA-dependent RNA polymerase activity
Molecular Functionzinc ion binding
Biological ProcessDNA-templated transcription
Biological Processinduction by virus of host autophagy
Biological Processproteolysis
Biological Processviral genome replication
Biological Processviral protein processing
Biological Processviral translational frameshifting

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    ORF1ab polyprotein

Organism names

  • Taxonomic identifier
  • Strain
    • USA/Utah 29625/2014
  • Taxonomic lineage
    Viruses > Riboviria > Orthornavirae > Pisuviricota > Pisoniviricetes > Nidovirales > Cornidovirineae > Coronaviridae > Orthocoronavirinae > Alphacoronavirus > Pedacovirus

Accessions

  • Primary accession
    A0A0E3KKA4

Subcellular Location

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane1056-1074Helical
Transmembrane1117-1136Helical
Transmembrane1196-1217Helical
Transmembrane1224-1245Helical
Transmembrane1257-1277Helical
Transmembrane1620-1638Helical
Transmembrane1879-1896Helical
Transmembrane1951-1979Helical
Transmembrane2429-2446Helical
Transmembrane2453-2473Helical
Transmembrane2493-2511Helical
Transmembrane2523-2541Helical
Transmembrane2561-2585Helical
Transmembrane2592-2612Helical

Keywords

PTM/Processing

Keywords

Family & Domains

Features

Showing features for domain, region.

Type
IDPosition(s)Description
Domain1-83Ubiquitin-like
Region103-137Disordered
Domain149-388Peptidase C16
Domain378-557Macro
Domain722-777Ubiquitin-like
Domain783-1043Peptidase C16
Domain1130-11943Ecto
Region1268-1358Y1
Domain1268-1608CoV Nsp3 Y
Region1272-1285ZF1
Region1318-1328ZF2
Region1359-1608CoV-Y
Region1507-1608Y4
Domain1994-2089Nsp4C
Domain2090-2391Peptidase C30
Domain2672-2754RdRp Nsp7 cofactor
Domain2755-2949RdRp Nsp8 cofactor
Domain2950-3057Nsp9 ssRNA-binding
Domain3058-3195ExoN/MTase coactivator
Domain3198-3447NiRAN
Domain3453-3551Nsp12 Interface
Domain3552-4119Nsp12 RNA-dependent RNA polymerase
Domain4120-4203CV ZBD
Domain4367-4728+RNA virus helicase C-terminal
Domain4788-5002ExoN
Domain5011-5232N7-MTase
Region5123-5137GpppA-binding
Domain5234-5294Nsp15 N-terminal oligomerization
Domain5295-5412AV-Nsp11N/CoV-Nsp15M
Domain5429-5569NendoU
Domain5573-5740Nidovirus-type SAM-dependent 2'-O-MTase

Sequence similarities

Belongs to the coronaviruses polyprotein 1ab family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Fragment
  • Length
    5,740
  • Mass (Da)
    639,259
  • Last updated
    2015-06-24 v1
  • Checksum
    88FEEBAE5ED0D420

Features

Showing features for non-terminal residue.

TypeIDPosition(s)Description
Non-terminal residue1

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KP202365
EMBL· GenBank· DDBJ
AKA88558.1
EMBL· GenBank· DDBJ
Genomic RNA

Similar Proteins

Disclaimer

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