A0A0E3JUD7 · A0A0E3JUD7_SACSO
- ProteinHomocitrate synthase
- GenelysS
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids461 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the aldol-type condensation of 2-oxoglutarate with acetyl-CoA to yield homocitrate. Carries out the first step of the alpha-aminoadipate (AAA) lysine biosynthesis pathway.
Catalytic activity
- acetyl-CoA + 2-oxoglutarate + H2O = (2R)-homocitrate + CoA + H+This reaction proceeds in the forward direction.
Cofactor
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Pathway
Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-alpha-aminoadipate from 2-oxoglutarate: step 1/5.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 12 | 2-oxoglutarate (UniProtKB | ChEBI) | |||
Binding site | 13 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 76 | 2-oxoglutarate (UniProtKB | ChEBI) | |||
Binding site | 136 | 2-oxoglutarate (UniProtKB | ChEBI) | |||
Binding site | 170 | 2-oxoglutarate (UniProtKB | ChEBI) | |||
Binding site | 198 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 200 | Mg2+ (UniProtKB | ChEBI) | |||
Active site | 292 | Proton acceptor | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 2-isopropylmalate synthase activity | |
Molecular Function | homocitrate synthase activity | |
Molecular Function | metal ion binding | |
Biological Process | L-leucine biosynthetic process | |
Biological Process | lysine biosynthetic process via aminoadipic acid |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameHomocitrate synthase
- EC number
- Short namesHCS
Gene names
Organism names
- Strains
- Taxonomic lineageArchaea > Thermoproteota > Thermoprotei > Sulfolobales > Sulfolobaceae > Saccharolobus
Accessions
- Primary accessionA0A0E3JUD7
Proteomes
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 4-259 | Pyruvate carboxyltransferase | |||
Sequence similarities
Belongs to the alpha-IPM synthase/homocitrate synthase family. Homocitrate synthase LYS20/LYS21 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length461
- Mass (Da)50,882
- Last updated2015-06-24 v1
- ChecksumE87E9E4CA21BA56F
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP011055 EMBL· GenBank· DDBJ | AKA74204.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP011056 EMBL· GenBank· DDBJ | AKA76902.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP011057 EMBL· GenBank· DDBJ | AKA79594.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP033235 EMBL· GenBank· DDBJ | AZF68684.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP033236 EMBL· GenBank· DDBJ | AZF71304.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP033237 EMBL· GenBank· DDBJ | AZF73924.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP033238 EMBL· GenBank· DDBJ | AZF76547.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP033239 EMBL· GenBank· DDBJ | AZF79155.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP033240 EMBL· GenBank· DDBJ | AZF81760.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP033241 EMBL· GenBank· DDBJ | AZF84335.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LT549890 EMBL· GenBank· DDBJ | SAI84565.1 EMBL· GenBank· DDBJ | Genomic DNA |