A0A0E1XAT9 · A0A0E1XAT9_STAAU
- ProteinLipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit GatD
- GenegatD
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids243 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
The lipid II isoglutaminyl synthase complex catalyzes the formation of alpha-D-isoglutamine in the cell wall lipid II stem peptide. The GatD subunit catalyzes the hydrolysis of glutamine to glutamate and ammonia. The resulting ammonia molecule is channeled to the active site of MurT.
Catalytic activity
- beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + L-glutamine + ATP + H2O = beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + L-glutamate + ADP + phosphate + H+
Pathway
Cell wall biogenesis; peptidoglycan biosynthesis.
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 94 | Nucleophile | |||
Binding site | 128 | substrate | |||
Active site | 189 | ||||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | carbon-nitrogen ligase activity on lipid II | |
Molecular Function | glutaminase activity | |
Biological Process | cell wall organization | |
Biological Process | cobalamin biosynthetic process | |
Biological Process | glutamine metabolic process | |
Biological Process | peptidoglycan biosynthetic process | |
Biological Process | regulation of cell shape |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameLipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit GatD
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Staphylococcaceae > Staphylococcus
Accessions
- Primary accessionA0A0E1XAT9
Proteomes
Interaction
Subunit
Forms a heterodimer with MurT.
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 5-196 | CobB/CobQ-like glutamine amidotransferase | |||
Region | 219-243 | Disordered | |||
Sequence similarities
Belongs to the CobB/CobQ family. GatD subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length243
- Mass (Da)27,444
- Last updated2015-05-27 v1
- ChecksumD37566871EFCA326
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
ACJA02000002 EMBL· GenBank· DDBJ | EFH95898.1 EMBL· GenBank· DDBJ | Genomic DNA |