A0A0D6KJW9 · A0A0D6KJW9_9CYAN
- ProteinDNA ligase
- GeneligA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids678 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.
Catalytic activity
Cofactor
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 35-39 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 84-85 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 116 | NAD+ (UniProtKB | ChEBI) | |||
Active site | 118 | N6-AMP-lysine intermediate | |||
Binding site | 139 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 178 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 297 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 321 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 415 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 418 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 433 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 438 | Zn2+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | DNA binding | |
Molecular Function | DNA ligase (NAD+) activity | |
Molecular Function | metal ion binding | |
Biological Process | base-excision repair, DNA ligation | |
Biological Process | DNA replication |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA ligase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Cyanobacteriota > Cyanophyceae > Nostocales > Tolypothrichaceae > Tolypothrix
Accessions
- Primary accessionA0A0D6KJW9
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 600-678 | BRCT | |||
Sequence similarities
Belongs to the NAD-dependent DNA ligase family. LigA subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length678
- Mass (Da)75,699
- Last updated2015-05-27 v1
- MD5 Checksum304DD24C8D916C0F55AAD575A50F8E3C
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AGCR01000067 EMBL· GenBank· DDBJ | EKE99735.1 EMBL· GenBank· DDBJ | Genomic DNA |