A0A0D2YG06 · FUB4_FUSO4

Function

function

Hydrolase; part of the gene cluster that mediates the biosynthesis of fusaric acid, a mycotoxin with low to moderate toxicity to animals and humans, but with high phytotoxic properties (PubMed:25372119).
L-aspartate is suggested as fusaric acid amino acid precursor that is activated and further processed to O-acetyl-L-homoserine by cluster enzymes aspartate kinase FUB3 and homoserine O-acetyltransferase FUB5, as well as enzymes of the primary metabolism (By similarity).
The polyketide synthase (PKS) FUB1 generates the triketide trans-2-hexenal which is presumptively released by the hydrolase FUB4 and linked to the NRPS-bound amino acid precursor by NAD(P)-dependent dehydrogenase FUB6 (By similarity).
FUB1, FUB4, and the non-canonical NRPS Fub8 may form an enzyme complex (By similarity).
Further processing of the NRPS-bound intermediate might be carried out by FUB6 and the sulfhydrylase FUB7, enabling a spontaneous electrocyclization to close the carbon backbone of fusaric acid (By similarity).
Dihydrofusaric acid is likely to be released via reduction by the thioester reductase (TR) domain of FUB8 whereupon the final oxidation to fusaric acid may (also) be performed by the FMN-dependent dehydrogenase FUB9 (By similarity).

Biotechnology

Fusaric acid is phytotoxic to plants such as cotton and banana (PubMed:20955724, PubMed:23922960).
It has been shown to induce programmed cell death in plants (PubMed:16868776, PubMed:23838885).
In addition to a mild toxicity to animals, fusaric acid exhibits acanthamoebicidal, antioomycete, and antimycobacterial activities (PubMed:17927749, PubMed:21811925, PubMed:22864988).

Pathway

Mycotoxin biosynthesis.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site93Charge relay system
Active site183Charge relay system
Active site243Charge relay system

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentnucleus
Molecular Functionhydrolase activity
Biological Processsecondary metabolic process

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Hydrolase FUB4
  • EC number
  • Alternative names
    • Fusaric acid biosynthesis protein 4

Gene names

    • Name
      FUB4
    • ORF names
      FOXG_15244

Organism names

Accessions

  • Primary accession
    A0A0D2YG06

Proteomes

Organism-specific databases

Subcellular Location

Phenotypes & Variants

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004373171-267Hydrolase FUB4

Interaction

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the AB hydrolase 3 family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    267
  • Mass (Da)
    30,977
  • Last updated
    2015-04-29 v1
  • Checksum
    A2B809EB23B3AA3D
MRFLCLHGYAFSVEVLQQQMEPITAHLPSDWEYEFLEAGMEPTELMLPNLKQVPKPNYSWYNFPYPEDVEEAYERLAAYVESEGPFDGIWGFSQGGSMAALLLLMHQAEHPDTPYPFKMAIFTSAFLPHSFDNGVISWDLTEKNTLEPTYLPGRIDVSHGKKLDWKKDLHTSIEYDMINAVKDELDFPVDLLLRWRPSDIPEKIPVPSVHVRGLKDHYSFVDESVYELFDPEMARKMTHRGGHNFPRYNEELVHFAELIIETVVSLH

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
DS231721
EMBL· GenBank· DDBJ
KNB17119.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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