A0A0D2YG02 · FUB7_FUSO4

Function

function

Sulfhydrylase; part of the gene cluster that mediates the biosynthesis of fusaric acid, a mycotoxin with low to moderate toxicity to animals and humans, but with high phytotoxic properties (PubMed:25372119).
L-aspartate is suggested as fusaric acid amino acid precursor that is activated and further processed to O-acetyl-L-homoserine by cluster enzymes aspartate kinase FUB3 and homoserine O-acetyltransferase FUB5, as well as enzymes of the primary metabolism (By similarity).
The polyketide synthase (PKS) FUB1 generates the triketide trans-2-hexenal which is presumptively released by the hydrolase FUB4 and linked to the NRPS-bound amino acid precursor by NAD(P)-dependent dehydrogenase FUB6 (By similarity).
FUB1, FUB4, and the non-canonical NRPS Fub8 may form an enzyme complex (By similarity).
Further processing of the NRPS-bound intermediate might be carried out by FUB6 and the sulfhydrylase FUB7, enabling a spontaneous electrocyclization to close the carbon backbone of fusaric acid (By similarity).
Dihydrofusaric acid is likely to be released via reduction by the thioester reductase (TR) domain of FUB8 whereupon the final oxidation to fusaric acid may (also) be performed by the FMN-dependent dehydrogenase FUB9 (By similarity).

Cofactor

pyridoxal 5'-phosphate (UniProtKB | Rhea| CHEBI:597326 )

Biotechnology

Fusaric acid is phytotoxic to plants such as cotton and banana (PubMed:20955724, PubMed:23922960).
It has been shown to induce programmed cell death in plants (PubMed:16868776, PubMed:23838885).
In addition to a mild toxicity to animals, fusaric acid exhibits acanthamoebicidal, antioomycete, and antimycobacterial activities (PubMed:17927749, PubMed:21811925, PubMed:22864988).

Pathway

Mycotoxin biosynthesis.

GO annotations

AspectTerm
Molecular Functionpyridoxal phosphate binding
Molecular Functiontransferase activity, transferring alkyl or aryl (other than methyl) groups
Biological Processtranssulfuration

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Sulfhydrylase FUB7
  • EC number
  • Alternative names
    • Fusaric acid biosynthesis protein 7

Gene names

    • Name
      FUB7
    • ORF names
      FOXG_15240

Organism names

Accessions

  • Primary accession
    A0A0D2YG02

Proteomes

Organism-specific databases

Phenotypes & Variants

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00004373381-433Sulfhydrylase FUB7
Modified residue211N6-(pyridoxal phosphate)lysine

Interaction

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the trans-sulfuration enzymes family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    433
  • Mass (Da)
    47,110
  • Last updated
    2015-04-29 v1
  • Checksum
    6C1F64DEABE346F0
MAEQVFQNFETLQLHAGYTPDPHTRSTAVPIYATSSYTFNDSAHGARLFGLKELGNIYSRLMNPTVDVFEKRIAALEGGIAAAATSSGQAAQFLTIATLAKAGDNIVASSHLYGGTYNQLNVLLPRFGIKTKFVRSGKLEDYAAAIDDQTRAIYVESMSNPDYVVPDFEGIAKIAHEHGIPLVVDNTLGAGGYYIRPIEHGADIVVHSATKWIGGHGTTIGGVIVDSGRFNWNKHSERFPEMVEPSPSYHGLKYWEAFGPATFITRIRVEMLRDIGACLSPFSAQQLLLGIETLGLRAERHAQNTEKLAKYFESSPNVSWVLWPGSESHPTYAQAKKYLTRGFGAMLSIGVKGDASAGSKVVDGLKLVSNLANVGDAKSLAIHPWSTTHEQLSEDERLASGVTEDMIRISVGIEHVDDIIADFEQSFQKAYGS

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
DS231721
EMBL· GenBank· DDBJ
KNB17098.1
EMBL· GenBank· DDBJ
Genomic DNA
DS231721
EMBL· GenBank· DDBJ
KNB17099.1
EMBL· GenBank· DDBJ
Genomic DNA
DS231721
EMBL· GenBank· DDBJ
KNB17100.1
EMBL· GenBank· DDBJ
Genomic DNA
DS231721
EMBL· GenBank· DDBJ
KNB17101.1
EMBL· GenBank· DDBJ
Genomic DNA
DS231721
EMBL· GenBank· DDBJ
KNB17102.1
EMBL· GenBank· DDBJ
Genomic DNA
DS231721
EMBL· GenBank· DDBJ
KNB17103.1
EMBL· GenBank· DDBJ
Genomic DNA
DS231721
EMBL· GenBank· DDBJ
KNB17104.1
EMBL· GenBank· DDBJ
Genomic DNA
DS231721
EMBL· GenBank· DDBJ
KNB17105.1
EMBL· GenBank· DDBJ
Genomic DNA
DS231721
EMBL· GenBank· DDBJ
KNB17106.1
EMBL· GenBank· DDBJ
Genomic DNA
DS231721
EMBL· GenBank· DDBJ
KNB17107.1
EMBL· GenBank· DDBJ
Genomic DNA
DS231721
EMBL· GenBank· DDBJ
KNB17108.1
EMBL· GenBank· DDBJ
Genomic DNA
DS231721
EMBL· GenBank· DDBJ
KNB17109.1
EMBL· GenBank· DDBJ
Genomic DNA
DS231721
EMBL· GenBank· DDBJ
KNB17110.1
EMBL· GenBank· DDBJ
Genomic DNA
DS231721
EMBL· GenBank· DDBJ
KNB17111.1
EMBL· GenBank· DDBJ
Genomic DNA
DS231721
EMBL· GenBank· DDBJ
KNB17112.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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