A0A0D2UEP9 · A0A0D2UEP9_GOSRA
- ProteinPyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta
- GenePFP-BETA
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids566 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalytic subunit of pyrophosphate--fructose 6-phosphate 1-phosphotransferase. Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis.
Catalytic activity
- beta-D-fructose 6-phosphate + diphosphate = beta-D-fructose 1,6-bisphosphate + H+ + phosphate
Cofactor
Activity regulation
Allosterically activated by fructose 2,6-bisphosphate.
Pathway
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Features
Showing features for binding site, site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 104 | diphosphate (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 198 | Mg2+ (UniProtKB | ChEBI); catalytic | ||||
Sequence: D | ||||||
Site | 199 | Important for catalytic activity and substrate specificity; stabilizes the transition state when the phosphoryl donor is PPi; prevents ATP from binding by mimicking the alpha-phosphate group of ATP | ||||
Sequence: D | ||||||
Site | 225 | Important for catalytic activity; stabilizes the transition state when the phosphoryl donor is PPi | ||||
Sequence: K | ||||||
Binding site | 226-228 | substrate | ||||
Sequence: TID | ||||||
Active site | 228 | Proton acceptor | ||||
Sequence: D | ||||||
Binding site | 265-266 | substrate; ligand shared between dimeric partners | ||||
Sequence: KY | ||||||
Binding site | 273-275 | substrate | ||||
Sequence: MGR | ||||||
Binding site | 334 | substrate | ||||
Sequence: E | ||||||
Binding site | 439-442 | substrate | ||||
Sequence: YEGR |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | 6-phosphofructokinase activity | |
Molecular Function | ATP binding | |
Molecular Function | diphosphate-fructose-6-phosphate 1-phosphotransferase activity | |
Molecular Function | metal ion binding | |
Biological Process | fructose 6-phosphate metabolic process | |
Biological Process | photosynthesis | |
Biological Process | response to glucose |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta
- EC number
- Short namesPFP
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Malvales > Malvaceae > Malvoideae > Gossypium
Accessions
- Primary accessionA0A0D2UEP9
Proteomes
Genome annotation databases
Subcellular Location
Interaction
Subunit
Tetramer of two alpha (regulatory) and two beta (catalytic) chains.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 96-409 | Phosphofructokinase | ||||
Sequence: KIGVVLSGGQAPGGHNVISGIFDYLQERAPGSTLYGFKGGPAGIMKGKYVELTADYIYPYRNQGGFDMICSGRDKIETPEQFKQAEETAAKLDLDGLVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPTSFGFDTACKIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVAAKKLTLKNVTDYMVDVICKRAELGYNYGVILIPEGLIDFIPEVQQLIAELNEILAHDVVDENGLWKKKLTDQSLKLFEFLPQAIQEQLMLERDPHGNVQVAKIETEKML |
Sequence similarities
Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Clade 'Long' sub-subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length566
- Mass (Da)61,567
- Last updated2015-04-29 v1
- Checksum4187F1885D6509B4
Computationally mapped potential isoform sequences
There are 6 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A0D2UEQ6 | A0A0D2UEQ6_GOSRA | PFP-BETA | 558 | ||
A0A0D2UGU1 | A0A0D2UGU1_GOSRA | PFP-BETA | 487 | ||
A0A0D2SV36 | A0A0D2SV36_GOSRA | PFP-BETA | 556 | ||
A0A0D2R9T6 | A0A0D2R9T6_GOSRA | PFP-BETA | 460 | ||
A0A0D2VBZ3 | A0A0D2VBZ3_GOSRA | B456_010G184300 | 480 | ||
A0A0D2VBZ8 | A0A0D2VBZ8_GOSRA | PFP-BETA | 502 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CM001749 EMBL· GenBank· DDBJ | KJB67269.1 EMBL· GenBank· DDBJ | Genomic DNA |