A0A0D1IQZ4 · A0A0D1IQZ4_STAAU

Function

function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
[2Fe-2S] cluster (UniProtKB | Rhea| CHEBI:190135 )

Note: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.
[2Fe-2S] cluster (UniProtKB | Rhea| CHEBI:190135 )

Note: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.
[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site61[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site65[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site68[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site104[2Fe-2S] cluster (UniProtKB | ChEBI)
Binding site137[2Fe-2S] cluster (UniProtKB | ChEBI)
Binding site197[2Fe-2S] cluster (UniProtKB | ChEBI)
Binding site267[2Fe-2S] cluster (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function2 iron, 2 sulfur cluster binding
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionbiotin synthase activity
Molecular Functioniron ion binding
Biological Processbiotin biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Biotin synthase
  • EC number

Gene names

    • Name
      bioB
    • ORF names
      CV021_14670
      , DQU51_05770
      , E1948_02100
      , E1948_12835
      , G0Z31_04255
      , GO941_02495
      , GQX52_12970
      , GZ111_009225
      , M1K003_0967
      , NCTC6133_03260
      , QU38_07880
      , SAMEA1466929_01128
      , SAMEA1531744_00538

Organism names

  • Taxonomic identifier
  • Organism
  • Strains
    • SA-120
    • M1K003
    • G703N2B1
    • pt014
    • Pt014
  • Taxonomic lineage
    Bacteria > Bacillota > Bacilli > Bacillales > Staphylococcaceae > Staphylococcus

Accessions

  • Primary accession
    A0A0D1IQZ4
  • Secondary accessions
    • A0A2P8SX58

Proteomes

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain43-269Radical SAM core

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    335
  • Mass (Da)
    37,545
  • Last updated
    2015-04-29 v1
  • Checksum
    0C7DAB4ECCB73A5B
MNLAKRILQGEQLTKETVLKIYEDTNIDTLDLLNEAYILRKHYFGKKVKLNMILNAKSGICPENCGYCGQSRDIKQKQRYALIPEEQIIDGAKVAHDNHIGTYCIVMSGRGPSDKEVDHISNTVRTIKSQHPQLKICACLGLTNDEQAKKLKSAGVDRYNHNINTSENYHDNVVTTHSYKDRTDTIELMKANNISPCSGVICGMGESNQDIVDMAFALKEMDADSIPINFLHPIKGTKFGSMDDLTPMKCLRIVALFRLINPTKEIRIAGGREVNLRSLQPLALKAANSIFVGDYLITGGQPNQLDYDMINDLGFEIDYDTCENKENKNDVSRAN

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BDVT01000003
EMBL· GenBank· DDBJ
GBV19987.1
EMBL· GenBank· DDBJ
Genomic DNA
JXIG01000626
EMBL· GenBank· DDBJ
KIT96963.1
EMBL· GenBank· DDBJ
Genomic DNA
JAIGOF010000002
EMBL· GenBank· DDBJ
MBX8593406.1
EMBL· GenBank· DDBJ
Genomic DNA
WPVZ01000171
EMBL· GenBank· DDBJ
MVL44365.1
EMBL· GenBank· DDBJ
Genomic DNA
JAAJIY010000008
EMBL· GenBank· DDBJ
NGK20731.1
EMBL· GenBank· DDBJ
Genomic DNA
JAANDN010000112
EMBL· GenBank· DDBJ
NUY69504.1
EMBL· GenBank· DDBJ
Genomic DNA
PGWZ01000558
EMBL· GenBank· DDBJ
PPJ69817.1
EMBL· GenBank· DDBJ
Genomic DNA
CP038850
EMBL· GenBank· DDBJ
QCT58191.1
EMBL· GenBank· DDBJ
Genomic DNA
UDJK01000003
EMBL· GenBank· DDBJ
SRC24046.1
EMBL· GenBank· DDBJ
Genomic DNA
UHAP01000001
EMBL· GenBank· DDBJ
SUK60294.1
EMBL· GenBank· DDBJ
Genomic DNA
UIOA01000002
EMBL· GenBank· DDBJ
SVF84605.1
EMBL· GenBank· DDBJ
Genomic DNA
QNXH01000001
EMBL· GenBank· DDBJ
TXL37062.1
EMBL· GenBank· DDBJ
Genomic DNA
CP152420
EMBL· GenBank· DDBJ
XAJ29692.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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