A0A0B5DN91 · A0A0B5DN91_9ACTN

  • Protein
    Probable dual-specificity RNA methyltransferase RlmN
  • Gene
    rlmN
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.

Miscellaneous

Reaction proceeds by a ping-pong mechanism involving intermediate methylation of a conserved cysteine residue.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Features

Showing features for active site, binding site.

Type
IDPosition(s)Description
Active site109Proton acceptor
Binding site129[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site133[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site136[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site184-185S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site218S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site241-243S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site317S-adenosyl-L-methionine (UniProtKB | ChEBI)
Active site360S-methylcysteine intermediate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionmetal ion binding
Molecular FunctionrRNA (adenine(2503)-C2-)-methyltransferase activity
Molecular FunctionrRNA binding
Molecular FunctiontRNA (adenine(37)-C2)-methyltransferase activity
Molecular FunctiontRNA binding
Biological ProcessrRNA base methylation
Biological ProcesstRNA methylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Probable dual-specificity RNA methyltransferase RlmN
  • EC number
  • Alternative names
    • 23S rRNA (adenine(2503)-C(2))-methyltransferase
    • 23S rRNA m2A2503 methyltransferase
    • Ribosomal RNA large subunit methyltransferase N
    • tRNA (adenine(37)-C(2))-methyltransferase
    • tRNA m2A37 methyltransferase

Gene names

    • Name
      rlmN
    • ORF names
      CP978_24235
      , SNOD_23925

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • ATCC 14899
  • Taxonomic lineage
    Bacteria > Actinomycetota > Actinomycetes > Kitasatosporales > Streptomycetaceae > Streptomyces

Accessions

  • Primary accession
    A0A0B5DN91

Proteomes

Subcellular Location

Keywords

PTM/Processing

Keywords

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain115-355Radical SAM core

Sequence similarities

Belongs to the radical SAM superfamily. RlmN family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    368
  • Mass (Da)
    40,332
  • Last updated
    2015-04-01 v1
  • Checksum
    A478C5EB6F0BBF61
MPKPGELTFVAPRGAKKPPRHLADLTPAERKEAVAEIGEKPFRAKQLSQHYFARYAHDPEQWTDIPAGSRARLQEALLPELMTVVRHLSTDQDTTRKTLWRLFDGTLVESVLMRYPDRVTMCISSQAGCGMNCPFCATGQAGLDRNLSTAEIVHQIVDGMRALRDGEIPGGPARLSNIVFMGMGEPLANYGRVVGAIRALTDPAPDGVGLSQRGITVSTVGLVPAIHRFADEGFKCRLAVSLHAPDDELRDTLVPVNTRWKVREVLDAGFGYVERSGRRLSIEYALIRDINDQAWRGDLLGRLLKGKPVHVNLIPLNPTPGSKWTASRPEDEKAFVEAIAAHGVPVTVRDTRGQEIDGACGQLAATER

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP009313
EMBL· GenBank· DDBJ
AJE42750.1
EMBL· GenBank· DDBJ
Genomic DNA
CP023747
EMBL· GenBank· DDBJ
QEV41250.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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