A0A0B5DHN0 · A0A0B5DHN0_9ACTN

  • Protein
    ATP-dependent 6-phosphofructokinase
  • Gene
    pfkA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.

Features

Showing features for binding site, site, active site.

Type
IDPosition(s)Description
Binding site10ATP (UniProtKB | ChEBI)
Binding site72-73ATP (UniProtKB | ChEBI)
Binding site102-105ATP (UniProtKB | ChEBI)
Binding site103Mg2+ (UniProtKB | ChEBI); catalytic
Site104Important for substrate specificity; cannot use PPi as phosphoryl donor
Binding site125-127substrate; ligand shared between dimeric partners; in other chain
Active site127Proton acceptor
Binding site162substrate; ligand shared between dimeric partners
Binding site169-171substrate; ligand shared between dimeric partners; in other chain
Binding site222substrate; ligand shared between dimeric partners; in other chain
Binding site266substrate; ligand shared between dimeric partners
Binding site272-275substrate; ligand shared between dimeric partners; in other chain

GO annotations

AspectTerm
Cellular Component6-phosphofructokinase complex
Molecular Function6-phosphofructokinase activity
Molecular FunctionAMP binding
Molecular FunctionATP binding
Molecular Functiondiphosphate-fructose-6-phosphate 1-phosphotransferase activity
Molecular Functionfructose-6-phosphate binding
Molecular Functionidentical protein binding
Molecular Functionmetal ion binding
Molecular Functionmonosaccharide binding
Biological Processcanonical glycolysis
Biological Processfructose 1,6-bisphosphate metabolic process
Biological Processfructose 6-phosphate metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    ATP-dependent 6-phosphofructokinase
  • EC number
  • Short names
    ATP-PFK
    ; Phosphofructokinase
  • Alternative names
    • Phosphohexokinase

Gene names

    • Name
      pfkA
    • ORF names
      CP978_05755
      , SNOD_05410

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • ATCC 14899
  • Taxonomic lineage
    Bacteria > Actinomycetota > Actinomycetes > Kitasatosporales > Streptomycetaceae > Streptomyces

Accessions

  • Primary accession
    A0A0B5DHN0

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homodimer or homotetramer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain2-298Phosphofructokinase

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    341
  • Mass (Da)
    36,477
  • Last updated
    2015-04-01 v1
  • Checksum
    09294B12865D1B98
MRIGVLTSGGDCPGLNAVIRSVVHRAVADHGDEVIGFRDGWKGLLECDYLKLDLDAVSGILARGGTVLGSSRVRPEHLRDGVERARGHVEELGLDGIIPIGGEGTLKAARLLADHGLPIVGVPKTIDNDIAVTDVTFGFDTAVGVATEALDRLKTTAESHQRVLIVEVMGRHTGWIALHSGTAAGAHAIVVPERPFDIEELAARVGERFSAGKRFAIVVAAEGAKPRPGTMDFDEGGKDIYGHERFAGIARQLSLELERRLGKEARPVILGHVQRGGTPTAYDRVLATRFGWHAVEAVHRGEFGRMTALRGTDIVTVPLADAVQTLKTVPPERYAEADCVL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP009313
EMBL· GenBank· DDBJ
AJE39522.1
EMBL· GenBank· DDBJ
Genomic DNA
CP023747
EMBL· GenBank· DDBJ
QEV38103.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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