A0A0B4VFI7 · A0A0B4VFI7_CHIKV

Function

function

Inactive precursor of the viral replicase, which is activated by cleavages carried out by the viral protease nsP2.

Catalytic activity

  • 4-O-(ADP-D-ribosyl)-L-aspartyl-[protein] + H2O = ADP-D-ribose + H+ + L-aspartyl-[protein]
    This reaction proceeds in the forward direction.
  • 5-O-(ADP-D-ribosyl)-L-glutamyl-[protein] + H2O = ADP-D-ribose + H+ + L-glutamyl-[protein]
    This reaction proceeds in the forward direction.
  • ADP-alpha-D-ribose 1''-phosphate + H2O = ADP-D-ribose + phosphate
    This reaction proceeds in the forward direction.
    EC:3.1.3.84 (UniProtKB | ENZYME | Rhea)
  • ATP + H2O = ADP + H+ + phosphate
    EC:3.6.4.13 (UniProtKB | ENZYME | Rhea)
  • ATP + RNA(n) = diphosphate + RNA(n)-3'-adenine ribonucleotide
    EC:2.7.7.19 (UniProtKB | ENZYME | Rhea)
  • GTP + S-adenosyl-L-methionine = N7-methyl-GTP + S-adenosyl-L-homocysteine
  • L-histidyl-[protein] + N7-methyl-GTP = diphosphate + N(tele)-(N7-methylguanosine 5'-phospho)-L-histidyl-[protein]
    This reaction proceeds in the forward direction.
  • a 5'-end diphospho-(purine-ribonucleoside) in mRNA + H+ + N(tele)-(N7-methylguanosine 5'-phospho)-L-histidyl-[protein] = a 5'-end (N7-methyl 5'-triphosphoguanosine)-(purine-ribonucleoside) in mRNA + L-histidyl-[protein]
  • a 5'-end triphospho-ribonucleoside in mRNA + H2O = a 5'-end diphospho-ribonucleoside in mRNA + H+ + phosphate
    This reaction proceeds in the forward direction.
    EC:3.6.1.74 (UniProtKB | ENZYME | Rhea)
  • a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-diphosphate + H+ + phosphate
    EC:3.6.1.15 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site1013For cysteine protease nsP2 activity
Active site1083For cysteine protease nsP2 activity

GO annotations

AspectTerm
Cellular Componentcytoplasmic vesicle membrane
Cellular Componenthost cell cytoplasmic vesicle membrane
Cellular Componenthost cell filopodium
Cellular Componenthost cell nucleus
Cellular Componenthost cell plasma membrane
Cellular Componentplasma membrane
Molecular FunctionATP binding
Molecular Functioncysteine-type peptidase activity
Molecular FunctionGTP binding
Molecular Functionmetal ion binding
Molecular FunctionmRNA methyltransferase activity
Molecular Functionpoly(A) RNA polymerase activity
Molecular Functionpolynucleotide 5'-phosphatase activity
Molecular Functionribonucleoside triphosphate phosphatase activity
Molecular FunctionRNA binding
Molecular FunctionRNA helicase activity
Molecular FunctionRNA-dependent RNA polymerase activity
Biological Process7-methylguanosine mRNA capping
Biological ProcessDNA-templated transcription
Biological Processmethylation
Biological ProcessmRNA modification
Biological Processproteolysis
Biological Processsymbiont-mediated suppression of host mRNA transcription via inhibition of RNA polymerase II activity
Biological Processviral RNA genome replication

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Polyprotein P1234
  • Alternative names
    • Non-structural polyprotein

Organism names

Accessions

  • Primary accession
    A0A0B4VFI7

Proteomes

Subcellular Location

Cell membrane
; Lipid-anchor
Cytoplasmic vesicle membrane
; Lipid-anchor
Cytoplasmic vesicle membrane
; Peripheral membrane protein
Host cell membrane
; Lipid-anchor
Host cytoplasmic vesicle membrane
; Lipid-anchor
Host cytoplasmic vesicle membrane
; Peripheral membrane protein
Host nucleus
Membrane
; Lipid-anchor
Membrane
; Peripheral membrane protein

Keywords

PTM/Processing

Keywords

Family & Domains

Features

Showing features for domain, compositional bias, region.

TypeIDPosition(s)Description
Domain28-259Alphavirus-like MT
Domain690-991+RNA virus helicase C-terminal
Domain1004-1327Peptidase C9
Domain1324-1493Macro
Compositional bias1778-1806Polar residues
Region1778-1808Disordered
Domain2228-2343RdRp catalytic

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    2,474
  • Mass (Da)
    275,818
  • Last updated
    2017-10-25 v2
  • Checksum
    0442CC91D4A699E7
MDPVYVDIDADSAFLKALQRAYPMFEVESRQVTPNDHANARAFSHLAIKLIEQEIDPDSTILDIGSAPARRMMSERKYHCVCPMRSAEDPERLANYARKLASAAGKVLDRNISGKIGDLQAVMAVPDTETPTFCLHTDVSCRQRADVAIYQDVYAIHAPTSLYHQAIKGVRVAYWVGFDTTPFMYNAMAGAYPSYSTNWADEQVLKAKNIGLCSTDLTEGRRGKLSIMRGKKLKPCDRVLFSVGSTLYPESRKLLKSWHLPSVFHLKGKLSFTCRCDTVVSCEGYVVKRITMSPGLYGKTTGYAVTHHADGFLMCKTTDTVDGERVSFSVCTYVPATICDQMTGILATEVTPEDAQKLLVGLNQRIVVNGRTQRNTNTMKNYLLPVVAQAFSKWAKECRKDMEDEKLLGVRERTLTCCCLWAFKKQKTHTVYKRPDTQSIQKVQAEFDSFVVPSLWSSGLSIPLRTRIKWLLSKVPKTDLIPYSGDTQEARDAEKEAEEEREAELTRVALPPLQAAQEDVQVEIDVEQLEDRAGAGIIETPRGAIKVTAQPIDHVVGEYLVLSPQTVLRSQKLSLIHALAEQVKTCTHSGRAGRYAVEAYDGRVLVPSGYAISPEDFQSLSESATMVYNEREFVNRKLHHIAMHGPALNTDEESYELVRAERTEHEYVYDVDQRRCCKKEEAAGLVLVGDLTNPPYHEFAYEGLKIRPACPYKIAVIGVFGVPGSGKSAIIKNLVTRQDLVTSGKKENCQEITTDVMRQRGLEISARTVDSLLLNGCNRPVDVLYVDEAFACHSGTLLALIALVRPRQKVVLCGDPKQCGFFNMMQMKVNYNHNICTQVYHKSISRRCTLPVTAIVSSLHYEGKMRTTNEYNKPIVVDTTGSTKPDPGDLVLTCFRGWVKQLQIDYRGYEVMTAAASQGLTRKGVYAVRQKVNENPLYASTSEHVNVLLTRTEGKLVWKTLSGDPWIKTLQNPPKGNFKATIKEWEVEHASIMAGICSHQMTFDTFQNKANVCWAKSLVPILETAGIKLNDRQWSQIIQAFKEDKAYSPEVALNEICTRMYGVDLDSGLFSKPLVSVYYADNHWDNRPGGKTFGFNPEAASILERKYPFTKGKWNINKQICVTTRRIEDFNPTTNIIPVNRRLPHSLVAEHRPVKGERMEWLVNKINGHHVLLVSGYNLALPTKRVTWVAPLGVRGADYTYNLELGLPATLGRYDLVVINIHTPFRIHHYQQCVDHAMKLQMLGGDSLRLLKPGGSLLIRAYGYADRTSERVICVLGRKFRSSRALKPPCVTSNTEMFFLFSNFDNGRRNFTTHVMNNQLNAAFVGQATRAGCAPSYRVKRMDIAKNDEECVVNAANPRGLPGDGVCKAVYKKWPESFKNSATPVGTAKTVMCGTYPVIHAVGPNFSNYSESEGDRELAAAYREVAKEVTRLGVNSVAIPLLSTGVYSGGKDRLIQSLNHLFTAMDSTDADVVIYCRDKEWEKKISEAIQMRTQVELLDEHISIDCDIVRVHPDSSLAGRKGYSTTEGVLYSYLEGTRFHQTAVDMAEIYTMWPKQTEANEQVCLYALGESIESIRQKCPVDDADASSPPKTVPCLCRYAMTPERVTRLRMNHVTSIIVCSSFPLPKYKIEGVQKVKCSKVMLFDHNVPSRVSPREYRSSLESAQEASTAMSLTHSRFDLSVDGEILPVPSDLDADAPALEPALDDGATHMLPSTTGNLAAVSDWVMSTVPIAPPRRRRGRNLTVTCDEREGNITPMASVRFFRAELCPVVRETAETRDTATSLQAPPSTATELNHPPTSFGAPSETFPITFGDFSEGEIESLSSELLTFGDFLPGEVDDLTDSDWSTCSDTDDELXLDRAGGYIFSSDTGPGHLQQKSVRQSVLPVNTLEEVHEEKCYPPKLDEAKEQLLLKKLQESASMANRSRYQSRKVENMKATIIQRLKRGCRLYLMSETPKVPTYRTIYPAPVYSPPINVRLSNPKSAVAACNEFLARNYPTVSSYQITDEYDAYLDMVDGSESCLDRATFNPSKLRSYPKQHAYHAPSIRSAVPSPFQNTLQNVLAAATKRNCNVTQMRELPTLDSAVFNVECFKNFACNQEYWEEFAASPIRITTENLTTYVTKLKGPKAAALFAKTHNLLPLQEVPMDRFTVDMKRDVKVTPGTKHTEERPKVQVIQAAEPLATAYLCGIHRELVRRLNAVLLPNVHTLFDMSAEDFDAIIAAHFKPGDTVLETDIASFDKSQDDSLALTALMLLEDLGVDHSLLDLIEAAFGEISSCHLPTGTRFKFGAMMKSGMFLTLFVNTLLNITIASRVLEDRLTKSACAAFIGDDNIIHGVVSDELMAARCATWMNMEVKIIDAAVSLKAPYFCGGFILHDTVTGTACRVADPLKRLFKLGKPLAAGDEQDEDRRRALADEVIRWQRTGLIDELEKAVYSRYEVQGISVVVMSMATFASSRSNFEKLRGPVITLYGGPK

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias1778-1806Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KP003813
EMBL· GenBank· DDBJ
AJD20059.2
EMBL· GenBank· DDBJ
Genomic RNA

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