A0A0B1U3S6 · A0A0B1U3S6_9BACT
- ProteinATP-dependent dethiobiotin synthetase BioD
- GenebioD
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids198 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA, also called 7,8-diammoniononanoate) to form a ureido ring.
Catalytic activity
- (7R,8S)-7,8-diammoniononanoate + ATP + CO2 = (4R,5S)-dethiobiotin + ADP + 3 H+ + phosphate
Cofactor
Pathway
Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 12-17 | ATP (UniProtKB | ChEBI) | ||||
Sequence: GIGKSY | ||||||
Binding site | 16 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Active site | 37 | |||||
Sequence: K | ||||||
Binding site | 41 | substrate | ||||
Sequence: T | ||||||
Binding site | 48 | ATP (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 48 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 104 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 104-107 | ATP (UniProtKB | ChEBI) | ||||
Sequence: ESAG | ||||||
Binding site | 164-165 | ATP (UniProtKB | ChEBI) | ||||
Sequence: ND |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | ATP binding | |
Molecular Function | dethiobiotin synthase activity | |
Molecular Function | magnesium ion binding | |
Biological Process | biotin biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameATP-dependent dethiobiotin synthetase BioD
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Thermodesulfobacteriota > Desulfovibrionia > Desulfovibrionales > Desulfovibrionaceae > Desulfovibrio
Accessions
- Primary accessionA0A0B1U3S6
Proteomes
Subcellular Location
Interaction
Structure
Sequence
- Sequence statusComplete
- Length198
- Mass (Da)21,381
- Last updated2015-03-04 v1
- Checksum359AAB51496FF344
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
JSEH01000007 EMBL· GenBank· DDBJ | KHK02763.1 EMBL· GenBank· DDBJ | Genomic DNA |