A0A0A7HD80 · A0A0A7HD80_9CAUD
- ProteinRNA ligase 1
- GenernlA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids378 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Involved in countering a host defense mechanism which, following viral infection, activates the host anticodon nuclease and shuts off viral translation. Repairs 5'-PO4 and 3'-OH groups in the cleaved host tRNA.
Catalytic activity
Cofactor
Note: Binds 2 magnesium ions that perform the catalytic activity via a two-metal mechanism. One of the catalytic Mg2+, which is coordinated by 5 water molecules, engages the lysine nucleophile and the ATP alpha phosphate while the Mg2+ orients the PPi leaving group.
Features
Showing features for binding site, active site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 38 | ATP (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Binding site | 55 | ATP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 76 | ATP (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Active site | 100 | N6-AMP-lysine intermediate | ||||
Sequence: K | ||||||
Binding site | 160 | ATP (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Site | 160 | Essential for RNA ligase activity | ||||
Sequence: E | ||||||
Binding site | 238 | ATP (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 240 | ATP (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Site | 244 | Essential for RNA ligase activity | ||||
Sequence: Y | ||||||
Binding site | 270 | Mg2+ (UniProtKB | ChEBI); catalytic | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | RNA ligase (ATP) activity | |
Biological Process | RNA repair |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameRNA ligase 1
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageViruses > Duplodnaviria > Heunggongvirae > Uroviricota > Caudoviricetes > Straboviridae > Tevenvirinae > Gaprivervirus > Gaprivervirus vr20
Accessions
- Primary accessionA0A0A7HD80
Proteomes
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 53-244 | T4 RNA ligase 1-like N-terminal | ||||
Sequence: ECRGIMFEMDDDGPVRIAARPMEKFFNLGENPLTMDINMDEVALVMTKADGSLVSTYMDQTNIAFKSKGSIKSSQAAEALQVFKQEHRIAFRERVTEITEAGYTCNMEYVAPTNRIVLPYQERDLILLNVRHNETGEYIPYQDLFKDAALRPFLVEAYDPTTIENVRDMKEIEGFVYELVDGRKFKVKTEWY | ||||||
Domain | 251-370 | T4 RNA ligase 1 C-terminal | ||||
Sequence: KDSVNNNQRLFEVVVAGGADDVIAMFTGDDYAREKVNAFTIAHLDYLSNTLGLLLDAQKQLAGKDRREFAIKGQELCKPYPGLFSVLMNTYQRPQETEQTIAQINNVFLKNHKLYIPVDY |
Sequence similarities
Belongs to the Tequatrovirus RNA ligase 1 family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length378
- Mass (Da)43,546
- Last updated2015-03-04 v1
- Checksum1A7B94F34937BDBC
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
KP007360 EMBL· GenBank· DDBJ | AIZ02296.1 EMBL· GenBank· DDBJ | Genomic DNA |