A0A0A7HD80 · A0A0A7HD80_9CAUD

Function

function

Involved in countering a host defense mechanism which, following viral infection, activates the host anticodon nuclease and shuts off viral translation. Repairs 5'-PO4 and 3'-OH groups in the cleaved host tRNA.

Catalytic activity

  • ATP + (ribonucleotide)n-3'-hydroxyl + 5'-phospho-(ribonucleotide)m = (ribonucleotide)n+m + AMP + diphosphate.
    EC:6.5.1.3 (UniProtKB | ENZYME | Rhea)

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 2 magnesium ions that perform the catalytic activity via a two-metal mechanism. One of the catalytic Mg2+, which is coordinated by 5 water molecules, engages the lysine nucleophile and the ATP alpha phosphate while the Mg2+ orients the PPi leaving group.

Features

Showing features for binding site, active site, site.

137850100150200250300350
TypeIDPosition(s)Description
Binding site38ATP (UniProtKB | ChEBI)
Binding site55ATP (UniProtKB | ChEBI)
Binding site76ATP (UniProtKB | ChEBI)
Active site100N6-AMP-lysine intermediate
Binding site160ATP (UniProtKB | ChEBI)
Site160Essential for RNA ligase activity
Binding site238ATP (UniProtKB | ChEBI)
Binding site240ATP (UniProtKB | ChEBI)
Site244Essential for RNA ligase activity
Binding site270Mg2+ (UniProtKB | ChEBI); catalytic

GO annotations

AspectTerm
Molecular FunctionATP binding
Molecular Functionmetal ion binding
Molecular FunctionRNA ligase (ATP) activity
Biological ProcessRNA repair

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    RNA ligase 1
  • EC number
  • Alternative names
    • Rnl1

Gene names

    • Name
      rnlA
    • ORF names
      VR20_238

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Viruses > Duplodnaviria > Heunggongvirae > Uroviricota > Caudoviricetes > Straboviridae > Tevenvirinae > Gaprivervirus > Gaprivervirus vr20

Accessions

  • Primary accession
    A0A0A7HD80

Proteomes

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain53-244T4 RNA ligase 1-like N-terminal
Domain251-370T4 RNA ligase 1 C-terminal

Sequence similarities

Belongs to the Tequatrovirus RNA ligase 1 family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    378
  • Mass (Da)
    43,546
  • Last updated
    2015-03-04 v1
  • Checksum
    1A7B94F34937BDBC
MIELYDNLMKLCAESDKGKFFYTDFVSPFETKFRIFSYNYASYSDWLLDDALECRGIMFEMDDDGPVRIAARPMEKFFNLGENPLTMDINMDEVALVMTKADGSLVSTYMDQTNIAFKSKGSIKSSQAAEALQVFKQEHRIAFRERVTEITEAGYTCNMEYVAPTNRIVLPYQERDLILLNVRHNETGEYIPYQDLFKDAALRPFLVEAYDPTTIENVRDMKEIEGFVYELVDGRKFKVKTEWYCALHHTKDSVNNNQRLFEVVVAGGADDVIAMFTGDDYAREKVNAFTIAHLDYLSNTLGLLLDAQKQLAGKDRREFAIKGQELCKPYPGLFSVLMNTYQRPQETEQTIAQINNVFLKNHKLYIPVDYQADVILPE

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KP007360
EMBL· GenBank· DDBJ
AIZ02296.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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