A0A0A7EPL0 · PIAL1_ARATH
- ProteinE4 SUMO-protein ligase PIAL1
- GenePIAL1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids847 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Together with MOM1 and PIAL2, regulates transcriptional gene silencing (TGS) independently of changes in DNA methylation (PubMed:27113777).
E4-type SUMO ligase that promotes SUMO chain formation in a SCE1-dependent manner and thus contributes to a pathway for proteolytic removal of sumoylation substrates (PubMed:25415977).
Involved in stress responses (e.g. osmotic, salt and abscisic acid ABA) and sulfur metabolism (PubMed:25415977).
E4-type SUMO ligase that promotes SUMO chain formation in a SCE1-dependent manner and thus contributes to a pathway for proteolytic removal of sumoylation substrates (PubMed:25415977).
Involved in stress responses (e.g. osmotic, salt and abscisic acid ABA) and sulfur metabolism (PubMed:25415977).
Pathway
Protein modification; protein sumoylation.
Features
Showing features for binding site.
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Molecular Function | ligase activity | |
Molecular Function | SUMO transferase activity | |
Molecular Function | zinc ion binding | |
Biological Process | positive regulation of sulfur metabolic process | |
Biological Process | protein sumoylation | |
Biological Process | regulation of gene silencing by regulatory ncRNA | |
Biological Process | response to abscisic acid | |
Biological Process | response to osmotic stress | |
Biological Process | response to salt stress |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameE4 SUMO-protein ligase PIAL1
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionA0A0A7EPL0
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
No obvious growth difference under standard greenhouse conditions (PubMed:25415977).
Altered sulfur metabolism (PubMed:25415977).
Reduced growth in high osmotic pressure (mannitol) and in response to abscisic acid (ABA), but enhanced growth and fitness in high salt (NaCl) condition (PubMed:25415977).
Abnormal steady state levels of SUMO conjugates in various conditions (PubMed:25415977).
Released transcriptional silencing of target genes (e.g. LTR, SDC, ROMANIAT5 and At5te35950) but unchanged DNA methylation levels; the expression level of these genes is higher in plants lacking both PIAL1 and PIAL2 (PubMed:27113777).
Altered sulfur metabolism (PubMed:25415977).
Reduced growth in high osmotic pressure (mannitol) and in response to abscisic acid (ABA), but enhanced growth and fitness in high salt (NaCl) condition (PubMed:25415977).
Abnormal steady state levels of SUMO conjugates in various conditions (PubMed:25415977).
Released transcriptional silencing of target genes (e.g. LTR, SDC, ROMANIAT5 and At5te35950) but unchanged DNA methylation levels; the expression level of these genes is higher in plants lacking both PIAL1 and PIAL2 (PubMed:27113777).
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000434951 | 1-847 | E4 SUMO-protein ligase PIAL1 | |||
Sequence: MVIPATSRFGFRAEFNTKEFQASCISLANEIDAAIGRNEVPGNIQELALILNNVCRRKCDDYQTRAVVMALMISVKSACQLGWFPERETQELLAIIDLMWNGFSCPENVTSCVNSPVTLISQVIERFYPCVKLGHILVSFEAKPESKMMMKDFHISKKMPHSPKQKVGLFVVRTEDISRSNCIVHPQGVSFLLNGKGIDKRVNISMESGPQLPTNVTALLNLGANLLQAIGCFGGSYLIAIAFMDVIPLPNKPLLKDYVHPEVVGSNSDCDIIEGPSRISLSCPISRTRIKLPVKGHVCKHLQCFDFWNYVNMNTRRPSWRCPHCNQSVCYTDIRVDQKLRKILEEVGRNAADVVISADGTWMVETENDEDVELVPETTHDHGDPNSFINLGPTVKNPARDENEMETSTQVEEHNPCLSEIQGPSNDTHRPASDYTMLNQSHTSTNTLPQLPRTLNAFDGQQFVNLPQVINTRDSPASQALPMTFSPTPSPQDILATNAANFGTSMPAAQSSQFQGSHVTSLGNCEGRTSDLMARWNHIYGRVQTQFPPAPLSHHHYSMQNQSPSPAQQRPVPSYIAHPQTFHVNYGENADQRWMPSSIAHPQTLPVNYGGNTNQRPIPSSIAHPQTLPVNYRGNTDHRSTPYSITHLQTLLNYGGNADQRPMPSSITNLQTLPATYGGYAHQRPMSSSITHPRTSPVNYGGTPDQRPMPSSITHPQTLPVSYGGTTDQILNPGGAMGQFSSREFMNLTPANTENWRPQSRMRGSVAPGTGYDHMIIHPTRPVHPQAQTPPAPLSTSYDGADEIQAFIGHPSYPVSNNETQAGTSSLPVAEGLGYSGSFWSMPPETW |
Proteomic databases
Expression
Tissue specificity
Expressed in leaves, stems and flowers, and, at low levels, in siliques and old leaves.
Gene expression databases
Structure
Family & Domains
Features
Showing features for region, zinc finger, repeat.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 113-271 | Interacting domain (IND), required for interaction with MOM1 and PIAL2 | ||||
Sequence: VNSPVTLISQVIERFYPCVKLGHILVSFEAKPESKMMMKDFHISKKMPHSPKQKVGLFVVRTEDISRSNCIVHPQGVSFLLNGKGIDKRVNISMESGPQLPTNVTALLNLGANLLQAIGCFGGSYLIAIAFMDVIPLPNKPLLKDYVHPEVVGSNSDCD | ||||||
Zinc finger | 268-349 | SP-RING-type | ||||
Sequence: SDCDIIEGPSRISLSCPISRTRIKLPVKGHVCKHLQCFDFWNYVNMNTRRPSWRCPHCNQSVCYTDIRVDQKLRKILEEVGR | ||||||
Repeat | 569-591 | 1 | ||||
Sequence: QRPVPSYIAHPQTFHVNYGENAD | ||||||
Region | 569-728 | 7 X 23 AA approximate tandem repeats | ||||
Sequence: QRPVPSYIAHPQTFHVNYGENADQRWMPSSIAHPQTLPVNYGGNTNQRPIPSSIAHPQTLPVNYRGNTDHRSTPYSITHLQTLLNYGGNADQRPMPSSITNLQTLPATYGGYAHQRPMSSSITHPRTSPVNYGGTPDQRPMPSSITHPQTLPVSYGGTTD | ||||||
Repeat | 592-614 | 2 | ||||
Sequence: QRWMPSSIAHPQTLPVNYGGNTN | ||||||
Repeat | 615-637 | 3 | ||||
Sequence: QRPIPSSIAHPQTLPVNYRGNTD | ||||||
Repeat | 638-659 | 4 | ||||
Sequence: HRSTPYSITHLQTLLNYGGNAD | ||||||
Repeat | 660-682 | 5 | ||||
Sequence: QRPMPSSITNLQTLPATYGGYAH | ||||||
Repeat | 683-705 | 6 | ||||
Sequence: QRPMSSSITHPRTSPVNYGGTPD | ||||||
Repeat | 706-728 | 7 | ||||
Sequence: QRPMPSSITHPQTLPVSYGGTTD |
Sequence similarities
Belongs to the PIAL protein ligase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
A0A0A7EPL0-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length847
- Mass (Da)93,475
- Last updated2015-03-04 v1
- ChecksumFF232EDE3000CCB1
A0A0A7EPL0-2
- Name2
- Differences from canonical
- 318-342: PSWRCPHCNQSVCYTDIRVDQKLRK → HHGAARI
Sequence caution
Features
Showing features for alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_057991 | 318-342 | in isoform 2 | |||
Sequence: PSWRCPHCNQSVCYTDIRVDQKLRK → HHGAARI |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
KP067953 EMBL· GenBank· DDBJ | AIY68679.1 EMBL· GenBank· DDBJ | mRNA | ||
AC000106 EMBL· GenBank· DDBJ | AAB70421.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
CP002684 EMBL· GenBank· DDBJ | AEE28366.1 EMBL· GenBank· DDBJ | Genomic DNA |