A0A0A7EG25 · A0A0A7EG25_9GAMM

Function

function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 1 zinc ion per subunit.

Features

Showing features for binding site.

1942100200300400500600700800900
TypeIDPosition(s)Description
Binding site567L-isoleucyl-5'-AMP (UniProtKB | ChEBI)
Binding site611ATP (UniProtKB | ChEBI)
Binding site905Zn2+ (UniProtKB | ChEBI)
Binding site908Zn2+ (UniProtKB | ChEBI)
Binding site925Zn2+ (UniProtKB | ChEBI)
Binding site928Zn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Functionaminoacyl-tRNA editing activity
Molecular FunctionATP binding
Molecular Functionisoleucine-tRNA ligase activity
Molecular FunctiontRNA binding
Molecular Functionzinc ion binding
Biological Processisoleucyl-tRNA aminoacylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Isoleucine--tRNA ligase
  • EC number
  • Alternative names
    • Isoleucyl-tRNA synthetase
      (IleRS
      )

Gene names

    • Name
      ileS
    • ORF names
      OM33_07480

Organism names

  • Taxonomic identifier
  • Strain
    • OCN003
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Alteromonadales > Pseudoalteromonadaceae > Pseudoalteromonas

Accessions

  • Primary accession
    A0A0A7EG25

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Monomer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, motif.

Type
IDPosition(s)Description
Domain27-646Aminoacyl-tRNA synthetase class Ia
Motif58-68'HIGH' region
Motif608-612'KMSKS' region
Domain691-846Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding
Domain903-930Zinc finger FPG/IleRS-type

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    942
  • Mass (Da)
    106,083
  • Last updated
    2015-03-04 v1
  • Checksum
    D310CD338D94E46A
MSDYKHTLNLPETQFPMRGNLAQREPQMLKSWYEKDLYGQIRQAKKGKKSFILHDGPPYANGNIHLGHSVNKILKDIIIKSKTLSGFDAPYVPGWDCHGLPIELQVEKKVGKPGKKVSVAEFREKCRDYAKKQVEGQKADFKRLGVFGDWDKPYLTMNFDFEANAIRVLGRIIKNGHLHKGSKPVHWCTDCGSALAEAEVEYQDKQSPAIDVKFTFVDEAALISKFNLADGHEGEGDISIVIWTTTPWTLPANRGVAVHEGLEYSLVQIETEQGKQRLVLASDLVKDAMDRYGVDKFHALGYCQGSDLELLQANHPFYDFTVPVILGEHVTTDSGTGAVHTAPGHGQEDFVVGQQYNLEVANPVGANGVYLPDTELFAGQHVFKANASVIDVLTEKGALMHHHALNHSYPHCWRHKTPIIFRATPQWFVSMDQANLRQDSLNEIKKTQWLPEWGESRIANMVEGRPDWCISRQRTWGVPIALFVDKDTGSLHPNTAELIEQVAVKVEEKGIQAWYDLDVTELLSAEDAEQYVKVQDTLDVWFDSGVTHACVVDAREELTGPADLYLEGSDQHRGWFMSSMMTSVAINGHAPYKQVLTHGFTVDQNGRKMSKSLGNVISPQDIMNKMGADILRLWVASTDYTAEMTVSDEIFKRSADRYRRIRNTARYLLANLNGFNPETDMIAADEMVALDRWILDRAAVLQDELVAAYDEYQMLVVTQKLMNFCTVELGSFYLDVIKDRQYTAKADSVARRSCQTALYHIAEAMTRWMAPIMSFTAQEIWQVLPGKRDEFVFTGEWYQGLVKADSAKLDNAFWQQLLEVRAEVNKVLEAARKEEKIGATLQAEVTLYVGKSLEEQLVALGDELRFVLLTSKAVVESKENRPEDAIASDIEGLFITVSATSAEKCERCWHYCDDVGTHEEHKDICGRCVTNVDGEGEVRQFA

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP009888
EMBL· GenBank· DDBJ
AIY65011.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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