A0A0A1U8W0 · A0A0A1U8W0_ENTIV

  • Protein
    Queuine tRNA-ribosyltransferase catalytic subunit 1
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product.

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site92Proton acceptor
Binding site92-96substrate
Binding site146substrate
Binding site190substrate
Binding site217substrate
Active site267Nucleophile
Binding site305Zn2+ (UniProtKB | ChEBI)
Binding site307Zn2+ (UniProtKB | ChEBI)
Binding site310Zn2+ (UniProtKB | ChEBI)
Binding site342Zn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functionmetal ion binding
Molecular FunctiontRNA-guanosine(34) queuine transglycosylase activity
Biological ProcesstRNA-guanine transglycosylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Queuine tRNA-ribosyltransferase catalytic subunit 1
  • EC number
  • Alternative names
    • Guanine insertion enzyme
    • tRNA-guanine transglycosylase

Gene names

    • ORF names
      EIN_525060

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • IP1
  • Taxonomic lineage
    Eukaryota > Amoebozoa > Evosea > Archamoebae > Mastigamoebida > Entamoebidae > Entamoeba

Accessions

  • Primary accession
    A0A0A1U8W0

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Interaction

Subunit

Heterodimer of a catalytic subunit and an accessory subunit.

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain14-373tRNA-guanine15 transglycosylase-like
Region248-254RNA binding
Region272-276RNA binding; important for wobble base 34 recognition

Sequence similarities

Belongs to the queuine tRNA-ribosyltransferase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    397
  • Mass (Da)
    44,202
  • Last updated
    2015-02-04 v1
  • Checksum
    819C4CE0DE0AFB5B
MSALSLTVNATDGRARQCTLHLPHGEVETPVFMPVGTKGSVKSLTYEQVTEMGCQILLANTYHMYLHPGVTVLKKSGGLHNYTKWNGNILTDSGGFQMVSLSETMSVSEEGVVFESIVDKKQINLPPEESIHIQQAIGSDIMMQLDDVVSSLTTGPRVSEAMERSVRWLGRCKKVYEETKDKQNLFGIIQGGLNADLRKKCLEGMVAVDTPGYAIGGLSGGEAKDDFWRMVDVSAQGLPETKPRYLMGVGYPVEMILCVLFGVDMFDCVYPTRTARFGTVFSDDGMVALKVNKYKDQYEVIDKNCDCLCCQKGNGLTKSALHFMFVNGAEEGTPVAQYLTHHNITYLFNLMRKYRVAIREKKAKVFAKEYIMRFYGGIQHVPAWMIDALKKAEVSFD

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KB206604
EMBL· GenBank· DDBJ
ELP89561.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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