A0A0A0EJD9 · A0A0A0EJD9_9GAMM
- ProteintRNA N6-adenosine threonylcarbamoyltransferase
- GenetsaD
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids340 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.
Catalytic activity
- adenosine37 in tRNA + L-threonylcarbamoyladenylate = AMP + H+ + N6-L-threonylcarbamoyladenosine37 in tRNA
Cofactor
Note: Binds 1 Fe2+ ion per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 111 | Fe cation (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 115 | Fe cation (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 137-141 | substrate | ||||
Sequence: LVSGG | ||||||
Binding site | 170 | substrate | ||||
Sequence: D | ||||||
Binding site | 183 | substrate | ||||
Sequence: G | ||||||
Binding site | 275 | substrate | ||||
Sequence: N | ||||||
Binding site | 303 | Fe cation (UniProtKB | ChEBI) | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | iron ion binding | |
Molecular Function | N(6)-L-threonylcarbamoyladenine synthase activity | |
Biological Process | tRNA threonylcarbamoyladenosine modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA N6-adenosine threonylcarbamoyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Lysobacterales > Lysobacteraceae > Lysobacter
Accessions
- Primary accessionA0A0A0EJD9
Proteomes
Subcellular Location
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 24-310 | Gcp-like | ||||
Sequence: LLAHALYSQIALHAEYGGVVPELASRDHVRKLLPLIRQALAEAGMTVGGIDGVAYTAGPGLVGALLVGAGVARSLAWALDIPAVAVHHMEGHLLAPLLEDDPLGRPEPPFVALLVSGGHTQLIAVDAIGRYRLLGETLDDAAGEAFDKTAKLMGLPYPGGPQLAMLAGQGRAGAYKFPRPMTNRPGLDFSFSGLKTQVMLAWRDGDQSDQTRADIARAFEEAVVDTLAIKCERALEAAGCETLVVAGGVGANLRLRAKLGEMAARRGGRVSFPRPAFCTDNGAMIAF |
Sequence similarities
Belongs to the KAE1 / TsaD family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length340
- Mass (Da)35,687
- Last updated2015-01-07 v1
- Checksum3A6A8934CD6463E8
Keywords
- Technical term